BillionToOne IPO BillionToOne is filing for IPO. They have an exciting “single molecule NGS” solution. If that was really single molecule that would be very exciting! After all we have two single molecule sequencers o...

aseq.substack.com/p/billiontoo...

Nautilus Bio Having looked at a few protein sequencing companies recently, I thought it might be worth checking in on Nautilus again.

aseq.substack.com/p/nautilus-bio

4 and 1 color systems are all being discontinued…

Element Pricing Disappeared? Element used to have a clear price list front and center on their website, it used to look like this, and it’s now gone:

aseq.substack.com/p/element-pr...

I believe all 1 and 4 color systems are now EOL, so it’s all be 2 color soon.

The problem is the MinION doesn’t seem to produce significant revenue and MinION revenue is going down not up. It would be unfortunate for users, but the correct strategy might be to cancel MinION/flongle and push more users to PromethION.

Bosch Building A Sequencer Too? I assume when people think “Bosch” they think power tools, engineering, industrial equipment.

aseq.substack.com/p/bosch-buil...

Oxford Nanopore Deploying $3B... Over in the Discord we were discussing how all sequencing companies except Illumina are losing money again.

aseq.substack.com/p/oxford-nan...

You’ve repeatedly asked to leave the ASeq Discord (where this comment was posted) in part because you quote without citing (like this), even when users have specifically request it.

Please leave the Discord. Sticking around in a forum you’ve been asked to leave is fairly creepy behavior.

One limitation of the MiSeq is that it only has a y stage not a full xy stage. Would either be limiting or requiring retrofitting I guess. But will be fun finding new applications for MiSeqs as they come on the market due to discontinuation!

Subscribe to ASeq Newsletter A Sequencing technology newsletter. Click to read ASeq Newsletter, by Nava Whiteford, a Substack publication.

The Roche SBX e-book available for free subscribers of the substack seems to be doing well. Considering books on Illumina, PacBio! Availavle when you subscribe at aseq.substack.com/subscribe

Subscribe to ASeq Newsletter A Sequencing technology newsletter. Click to read ASeq Newsletter, by Nava Whiteford, a Substack publication.

Wrote a short ebook on Roche’s SBX Nanopore sequencing approach. Available to free subscribers here: aseq.substack.com/subscribe

The PacBio Thesis No, not that kind of thesis… this kind of thesis:

aseq.substack.com/p/the-pacbio...

I bought one of these up a while back. If there’s anything I can help with give me a shout (new@sgenomics.org).

They showed 800bp read length average on Simplex and 350bp on duplex in the Webinar (which is full runs, but maybe cherry picked).

The preprint is 4 years out of date.

They showed single reads in the 2Kb range. One run with a simplex 800bp average IIRC. My guess is they can probably get a reasonable population at 2Kb currently but we don’t yet have good data from Roche on where the read length tops out.

The preprint is 2020 data. The current data they presented in the webinar was >99% accuracy. IIRC 99.3% raw. Duplex lets you filter a lot of those errors getting you to Q39 on concordant bases they say.

Confused, it’s already been installed and used at the Broad and another institute. Shows ~Q20 average on simplex Q39 on concordant duplex bases.

Can’t do modifications like ONT, but they’ve suggest they may be able to do something (seems hard to me!)

Happy Monday! Let's start this week with fairy tales!

ONCE UPON A TIME extracting high molecular weight (HMW) DNA was difficult. But now, with @pacbio.bsky.social Nanobind extraction kits. you get HMW DNA in, and beautiful long reads -> genome assemblies out! 💫⭐🧪🧬

www.pacb.com/products-and...

All The DNA Sequencing Companies Shawn has a really good list of current (and dead) DNA sequencing companies. I also had a list which I stopped updating in 2020.

All The DNA Sequencing Companies
open.substack.com/pub/aseq/p/a...

Acorn Genetics Acorn Genetics

aseq.substack.com/p/acorngenet...

Law Enforcement And Genealogy Databases Yesterday we talked about Microarrays, arrays appear to retain a strong market in genealogy (ancestry.com/23andMe).

aseq.substack.com/p/law-enforc...

Anti-competitive Illumina Someone sent me the following email in response to my article on possible Illumina sanctions, I thought it might be interesting the review and explore the points mentioned, breaking them down one-by-o...

aseq.substack.com/p/anti-compe...

Founders Fund Partner Did A Nazi Salute... This article has been updated

I’ve been writing about eugenics and investors doing Nazi salutes… your day sounds better:

bitsof.bio/p/nucleus-ge...

A Little More Roche Information... Alex Dickinson appears to have had an NDA’d briefing with Roche about their new nanopore sequencer and posted his thoughts over on LinkedIn. For what it’s worth, I declined an invite to preview the Ro...

aseq.substack.com/p/a-little-m...

How To Respond To Roche Any sequencing data coming from Roche on the 20th February could be an extinction level event for the DNA sequencing industry.

aseq.substack.com/p/how-to-res...

JPM In Review JPM is over and we’ve had some time to digest.

aseq.substack.com/p/jpm-in-rev...

Scale Versus Parse I’ve been poking around in the Scale v Parse/Roche lawsuit documents.

aseq.substack.com/p/scale-vers...

The Last Few Years In DNA Sequencing Almost 4 years ago I wrote an article called “The Next Few Years In DNA Sequencing”. I thought it was time to review and see how I did.

aseq.substack.com/p/the-last-f...

The 10X Chromium Controller Pneumatic System Last time I looked at the Chromium Controller estimating its BOM cost at <$5000. This time I wanted to further document the pneumatic system which forms the core of the Chromium Controller. You can se...

I then fully documented the Chromium pneumatic system, this was also fun!

aseq.substack.com/p/the-10x-ch...