Siegel Lab

Welcome to Munich. Wir excited to have you join our group.🎉

Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ - Nature Communications A nanoscale 3D DNA tracing workflow visualizes Cohesin-dependent loops in single, structurally well-preserved cells. Computer simulations based on the tracing data give further insight into how Cohesi...

Our new non-denaturing nanoscale 3D DNA tracing technology provides single-cell (and sister-chromatid specific!) readout of chromatin structure with 10kb genomic resolution - Check it out!

www.nature.com/articles/s41...

Congratulations to Kai and Oyvind!!

We’re very excited about the potential of IACS for advancing infectious disease research and believe this review from the Goda lab and its collaborators in @natrevbioeng.nature.com highlights its many powerful applications. Check out the full paper here: www.nature.com/articles/s44...

We found a new asymmetry in the large-scale chromosome structure: sister chromatids are systematically shifted by hundreds of kb in the 5′→3′ direction of their inherited strands! The work was led by Flavia Corsi, in close collaboration with the Daniel Gerlich lab.
www.biorxiv.org/content/10.1...
1/

Next generation genetic screens in kinetoplastids Abstract. The genomes of all organisms encode diverse functional elements, including thousands of genes and essential noncoding regions for gene regulation

We’re excited to share our review: "Next-generation genetic screens in kinetoplastids", now published in NAR!
It covers a range of perturbation methods—from RNAi to prime editing—as well as emerging assay technologies like scRNA-seq and image-activated cell sorting.
academic.oup.com/nar/article/...

Are you based in the U.S. 🇺🇸 or Canada 🇨🇦 and thinking of doing research in Europe?

ERC grants offer up to €4.5 million to support groundbreaking, investigator-driven frontier research in all fields.

Watch the dedicated info session for researchers in your region👉 youtu.be/92QYink1Lik

#ChooseEurope

New online! RNA splicing — a central layer of gene regulation

Chromatin loops are an ancestral hallmark of the animal regulatory genome - Nature The physical organization of the genome in non-bilaterian animals and their closest unicellular relatives is characterized; comparative analysis shows chromatin looping is a conserved feature of genome architecture and spatial genome regulation emerged early in animal evolution.

Nature research paper: Chromatin loops are an ancestral hallmark of the animal regulatory genome

https://go.nature.com/4kcNObU

These boxes are not moving. A mind-bending optical illusion by Japanese artist Jagarikin.

Defining the chromatin-associated protein landscapes on Trypanosoma brucei repetitive elements using synthetic TALE proteins Kinetoplastids, such as Trypansoma brucei, are eukaryotes that likely separated from the main lineage at an exceptionally early point in evolution. Consequently, many aspects of kinetoplastid biology ...

Our latest collaborative work with Keith Matthews’ lab using synthetic DNA binding proteins to explore the chromatin protein contexts of repetitive sequences in Trypanosoma brucei in now on bioRxiv - thanks to all involved! 2025.04.22.649942; doi: doi.org/10.1101/2025...

Very much looking forward to it.

Register for the excellent Epigenetics Gordon Research Conference Barcelona 10-15 Aug25. Theme: Epigenetic Variation: Mechanisms & Impact Across Systems. **FUNDING AVAILABLE to support attendance** ~15 talks to be chosen from submitted abstracts, so upload yours ASAP:
www.grc.org/epigenetics-...

Congratulations.

Great job!

An ISWI-related chromatin remodeller regulates stage-specific gene expression in Toxoplasma gondii Nature Microbiology - Toxoplasma gondii evolved two ISWI remodellers, TgSNF2h and TgSNF2L. TgSNF2h forms a complex that insulates active genes, regulates MORC-driven sexual commitment and ensures...

In the silence of chromatin, Toxoplasma whispers change.
TgSNF2h sculpts the genome - MORC in the backseat.
Some genes wake. Others sleep.
A dance of nucleosomes shaping space for life parasite stages to unfold.

Now in Nature Microbiology:
rdcu.be/ehkWp

Here is-🥳🥳😇 tremendous effort by @palmrinmoy.bsky.social brilliantly supported by @tamas-schauer.bsky.social &super help by federico,iliya,tsune,adam,@mamartirenom.bsky.social

Very happy to see this come to fruition- Look forward to feedback from colleagues !

More 👉 www.cell.com/cell/fulltex...

Great work.

thanks a lot.

This work was a great collaboration with the Colomé-Tatché and Mugnier labs.
@bmc-lmu.bsky.social
@www.helmholtz-munich.de
@johnshopkinssph.bsky.social
@uoe-eid.bsky.social

To evade the host immune response, many pathogens use antigenic variation, a strategy that involves periodic, semi-predictable switching of antigen expression.
Using a highly sensitive scRNA-seq approach, we have now discovered genomic features that control antigen selection mechanisms in T. brucei

If you are interested in antigenic variation, you may have wondered how parasites ‘select’ which antigen to express next.

Our manuscript, just published in Nature, provides some answers!

www.nature.com/articles/s41...

@lmumuenchen.bsky.social
@erc.europa.eu a.eu
@nature.com

#AidJobs: We are hiring, here is your chance to work with us.

International Secretary General: bit.ly/42VhaWN

Deputy Logistics Coordinator: bit.ly/4gIVDUf

Tembo Support Officer: bit.ly/4gJ9WIK

5 Nutritionists- bit.ly/3CVtzPW

Finance and Human Resources Manager: bit.ly/3QjZLzo

Hope we can join this meeting again this year.

Save The Date : The Next EMBO Workshop « Host-parasite relationship: from mechanisms to control strategies », organized by @ParaFrap, will be held October 5-8, 2025 @EMBOevents #Science #Parasite #France

these are beautiful images!

Expansion microscopy identifies novel asymmetric pore proteins. The images show proExM of cell lines co-expressing epitope-tagged versions of NUP76 (top; magenta), NUP149 (middle; magenta), or NUP140 (bottom; magenta) in combination with NUP110 (green). Images were deconvolved (20 iterations for NUP149 and NUP76; 60 iterations for NUP140) and single planes of the nuclei are shown.

How is transport directionality achieved through the #trypanosome nuclear pore complex? The current model is symmetrical, but this study reveals that the trypanosome #NuclearPore has an asymmetrical architecture, with conserved hubs that direct mRNA transport 🧪 @plosbiology.org plos.io/3Q36QV2

Please RS/RT. #GordonConference "Histone and DNA Modifications" Program is now online 🍾 #epigenetics #chromatin #histones 20.-25. July. Il Ciocco 🇮🇹
Register now: ➡️ shorturl.at/AtAjz
Exciting speaker lineup, many slots for talks selected from abstracts

I’m very much looking forward to attending the symposium and to celebrate the career of Mark Carrington.

Has Bluesky replaced X for scientists? Take Nature’s poll The research community has flocked to the social-media platform Bluesky. Tell us about your experience.

We have a feeling you'll be interested in this one.

Nature is keen to find out how scientists are using Bluesky and whether it has become their go-to social-media platform. Take the survey! 🧪

That is why we left.