Kristian Frandsen

PSA: If you're trying to run the Boltz ColabFold notebook and finding that it's crashing with this error, it's because you need to switch to an A100 GPU; the notebook no longer works with T4/L4 GPUs

FEBS Press The fungal lytic polysaccharide monooxygenase LsAA9A binds chloride in its crystallization mother liquor, leading to LsAA9A inhibition. Other halides except for fluoride inhibit LsAA9A activity at hi...

Here we present that anions and citrate inhibit #LPMO by directly chealating the active-site copper, (high-resolution crystal structure, PDB 8S3F).
#glycotime

febs.onlinelibrary.wiley.com/doi/10.1111/...

A claudin5-binding peptide enhances the permeability of the blood-brain barrier in vitro A claudin5 binding peptide enhances transport across the blood-brain barrier by transiently permeabilizing tight junctions.

Possible new way to increase BBB permeability transiently? At least in vitro…
www.science.org/doi/10.1126/...

Molecular architectures of centrosomes in C. elegans embryos visualized by cryo-electron tomography Tollervey et al. use cryo-electron tomography to capture 3D snapshots of centrosomes at molecular resolution in their near-native state inside embryonic C. elegans cells in different stages of cell division. They characterize structural features of centrioles during their assembly and of a disordered matrix that nucleates and organizes mitotic spindle microtubules.

Mahamid and Woodruff labs use cryo-ET to look at mitotic centrosomes: Stunned to see the pericentriolar matrix taking shape, and what seem to be ‚S size’ gamma-tubulin complex oligomers capping the end of 11-pf microtubules.

www.cell.com/developmenta...

First short talk by Eva Smorodina from University of Oslo.
Structural modeling of antibody variant epitope specificity with complementary experimental and computational techniques.

YouTube video by Nobel Prize 2024 Nobel Prize lectures in chemistry | David Baker, Demis Hassabis and John Jumper

A great day for computational structural biology!

2024 Nobel Prize lectures in chemistry by David Baker, Demis Hassabis and John Jumper:

www.youtube.com/watch?v=HnT1...

🧪🧬

one of the 3D structures of a tetrantennary, bisected N-glycan represented with sticks and a transparent surface to indicate its molecular volume. SNFG symbols are used to represent each monosacharide

Fancy glycan of the week 🍬: here ⬇️ is THE fanciest tetrantennary+SDa antigen bisected N-glycan I have ever seen! Found in bovine pregnancy-associated glycoproteins (PAGs) during midpregnancy, see doi.org/10.1093/glyc...

This (and its core-fucosylated companion) will be soon on glycoshape.org 😀👍

And the main conclusions for this years multimer predictions

Stereochemistry in the disorder–order continuum of protein interactions Nature - Studies on protein–protein interactions using proteins containing d- or l-amino acids show that stereoselectivity of binding varies with the degree of disorder within the complex.

Have you looked in the mirror recently? We took a good look at stereochemistry and how D- and L-ligands bind ... its all about disorder! Exited to share a true REPIN collaborative work led by @estellaan.bsky.social, A. Due and J. Olsen. Thanks for support NNF and DFF.
rdcu.be/d1F6q

🚨 New from PDBe!

Check out the revamped PDBeChem service with enhanced Ligand Pages!

🔍 Explore detailed ligand data, interaction stats, bound structures, related ligands, and more—all in one place.

👉 Read more in the news:
https://buff.ly/4g8eF6E

The paper ”Stereochemistry in the disorder–order continuum of protein interactions” from my colleagues @estellaan.bsky.social, Johan Olsen, @bbkrage.bsky.social et al is now out:
doi.org/10.1038/s415... 🍝 🧪 🧬 🧶

#GoodThingsComeToThoseWait Sorry it's taking us a while. Promise that the wait will be well worth it! #OpenFold3 Love all the great work that's already been done #HelixFold3 #Ligo #Chai1 #Protenix #Boltz1

https://www.embl.org/about/info/course-and-conference-office/events/stb24-01/#vf-tabs__section-programme

Really looking forward to welcoming & meeting the participants of our #EMBOStructBio course this morning!

Many of our speakers/tutors are here:
@moalquraishi.bsky.social
@sykhalid.bsky.social
@ezgikaraca.bsky.social
@amjjbonvin.bsky.social
@gchojnowski.bsky.social
@drmelissagraewert.bsky.social

If you like crystallography💎 (who doesn't) and the bitter-sweet pain of hard-to-work-with-ligands😭, then check out our latest publication,👇
“Structural and biochemical analysis of ligand binding in yeast Niemann–Pick type C1–related protein”
1/

Thrilled to announce Boltz-1, the first open-source and commercially available model to achieve AlphaFold3-level accuracy on biomolecular structure prediction! An exciting collaboration with Jeremy, Saro, and an amazing team at MIT and Genesis Therapeutics. A thread!

Proud of new work tinyurl.com/yhz4c6ry from the lab by Zhicheng (Chen) Cui using single-particle cryo-EM on liposomes to show how mTORC1 integrates nutrient and growth factor signaling at lysosomes, collab. w/
G. Napolitano,A. Esposito, and A. Ballabio.