Carmen.Gilgas

CaMKII nucleates an osmotic protein supercomplex to induce cellular bleb expansion - The EMBO Journal Blebs are membrane protrusions formed when localized regions of the plasma membrane detach from the actin cortex, enabling outward expansion driven by intracellular pressure. These structures play cri...

Is cortical actin the sole driver of cellular membrane bleb expansion?
Junichi Ikenouchi and colleagues implicate also calcium-dependent CaMKII supercomplex reorganization, generating local osmotic pressure to cause water influx for bleb growth
link.springer.com/article/10.1...

For those interested in topological analysis of biomolecular condensates!

Article 1: link.springer.com/article/10.1...
Article 2: www.sciencedirect.com/science/arti...

#Topology #Condensate #PhaseSeparation #Aggregation #ChemSky #Biophysics #Polymer #Nanoscience

Hay quienes, como los agujeros negros, carecen de luz propia. Paradójicamente, son los objetos más brillantes del universo.

La manipulación es la herramienta de quien carece de virtudes.
Quien posee integridad, honestidad o carisma genuino, persuade mediante la confianza y la evidencia.

How did life get multicellular? Five simple organisms could have the answer Single-celled species that often stick together in colonies have researchers rethinking the origin of animals.

For some three billion years, unicellular organisms ruled Earth. Then, around one billion years ago, a new chapter of life began

go.nature.com/3JyRV4S

Even so, the mitotic mechanism underlying the disaggregation process remains unclear

Might this indicate an unrecognised structural link between the nucleus and the ER?

It also remains unclear why an NLS reduces aggregation—perhaps by bypassing ER entry. BiP’s access to these nuclear aggregates is equally perplexing.

How these aggregates enter the nucleus remains uncertain, given their size exceeds the nuclear pore limit and their surrounding membrane suggests an endoplasmic reticulum origin.

Clearance of protein aggregates during cell division ER aggregates confined in the nucleus are cleared via ER reorganization when cells progress through mitosis and cytokinesis.

Misfolded proteins targeted to the endoplasmic reticulum unexpectedly form nuclear aggregates enclosed by a thin ER membrane. These aggregates are cleared following mitosis via CDK1 inactivation and BiP-mediated disaggregation.

elifesciences.org/articles/96675

Could mitosis clear misfolded proteins?

Of course, active processes are at play, yet the forces driving cellular dynamics extend far beyond our current understanding. We still lack a unified theory capable of accounting for the full spectrum of cellular behaviors and dynamics.

Inevitably, we represent cells in ℝ², within clearly defined, finite boundaries, devoid of gravitational effects. We often treat proteins as discrete units, whose assembly we model as though it were a mechanical assembly line.

Just some thoughts spiraling today...We have long regarded the cell as a discrete system — and rightly so, but what if that assumption is limiting? more akin to a Möbius strip of membranes and coexisting liquid phases?

De novo lipid synthesis and polarized prenylation drive cell invasion through basement membrane
pubmed.ncbi.nlm.nih.gov/39007804/

Calibrated ribosome profiling assesses the dynamics of ribosomal flux on transcripts - Nature Communications Ribosome profiling faces issues with rRNA contamination and measurements of ribosome numbers on transcripts. Here, the authors develop Ribo-FilterOut and Ribo-Calibration, methods which can be used to...

"these approaches estimate the number of ribosomes on a transcript, the translation initiation rate, and the overall number of translation events before its decay, all in a genome-wide manner" #rnasky #ribosome www.nature.com/articles/s41...

Two scutoids... a gran one and a yellow one.

Graphical abstract of the paper. We infer Forces from geometry to find that scutoids are necessary to form epithelial tubes.

So... Can be animals without #scutoids?
This a difficult question to answer, you cannot get mutants without scutoids.
So we designed a computational model to demonstrated that it is not possible to form epithelial tubes without apico-basal intercalations.
www.csbj.org/article/S200...

A figure depicting the concept of cancer attractor states within Waddington's landscape, and how gene regulatory network dynamics influence these states.

Genome sequencing & single-cell transcriptomics continue to produce findings that challenge the idea that #cancer is purely a genetic disease. This Essay delves into #omics data that questions the somatic mutation theory & presents alternative theories based on GRNs & tissue fields.🧪
plos.io/3DRtAEW

Study maps RNA polymerase II across 10 cancers, unveiling hypertranscription and its link to poor outcomes. Key: HER2 amplification! #CancerResearch PMID:39946483, Science 2025, @ScienceMagazine https://doi.org/10.1126/science.ads2169 #Medsky #Pharmsky #RNA 🧪

The Translation Termination Factor eRF3 Interacts Sequentially with eRF1 and ABCE1 on the Ribosome https://www.biorxiv.org/content/10.1101/2025.02.05.636767v1

🔸️🔸️🔸️🔹️CRISPR studies show that knocking out certain dark proteins affects cell survival, suggesting some have functional roles.

🔸️🔸️🔸️AI tools like AlphaFold often struggle to predict their structure, as many lack well-folded domains

🔸️🔸️At times playing a essential role, at others may be transient or quickly degraded, many are abundant in cancer cells.

🔸️These proteins are typically small, often fewer than 100 amino acids + not well conserved across species

Beyond known protein-coding genes, ribosomes are actively translating thousands of previously unrecognized dark proteins 🦹‍♀️🦹‍♂️🧟

👇👇👇👇

Theory of Computation | Mathematics | MIT OpenCourseWare This course emphasizes computability and computational complexity theory. Topics include regular and context-free languages, decidable and undecidable problems, reducibility, recursive function theory...

Highly recommend Sipser's @mitofficial.bsky.social Theory of Computation course to anyone interested in computation. Thorough and clear introduction. Plus, it’s FREE and available online! 🌐📚

ocw.mit.edu/courses/18-4...

#ComputerScience #TheoryOfComputation #OnlineLearning

Inverse blebs operate as hydraulic pumps during mouse blastocyst formation Nature Cell Biology - Schliffka et al. show that in the early mouse embryo, hemispherical intrusions, or inverse blebs, grow into cells at cell–cell adhesion sites in response to luminal...

#NCB2024
In September, @maitrejl.bsky.social, Schliffka, Mukherjee & co showed that in the early mouse #embryo inverse blebs grow into cells at cell–cell adhesion sites in response to luminal fluid accumulation and pressure build-up.
rdcu.be/d4uCu
www.nature.com/articles/s41...

🔸️🔸️🔸️🔹️🔹️🔹️Science has been reduced to a corrosive environment, so consider carefully whether your contributions are helping to dismantle this fragile ecosystem or to preserve it.

🔸️🔸️🔸️🔹️🔹️This comes across as mere bragging and boasting, with little to do with the actual pursuit of science. Before posting, perhaps it is worth reflecting on the original purpose behind what was supposed to be a humble career.