Matthew Macauley

Siglec-6 mediates the uptake of extracellular vesicles through a noncanonical glycolipid binding pocket - Nature Communications Siglec-glycolipid interactions are often studied outside the context of a lipid bilayer. Here, the authors combine a variety of chemical biology techniques to demonstrate a unique and physiologically ...

The link to our original study seems to be broken, so here it is: www.nature.com/articles/s41...

Schematic representation of the 14 human siglecs, 4 evolutionarily conserved on the left, with low sequence similarity, and 10 on the right sharing a higher sequence similarity to CD33. Siglec-6 is highlighted in yellow as it is the focus of this work. This is Figure 1 in the paper

When glycan recognition is linked to immune response, you need fine tuning! 😎

Here ⬇️ we show how human Siglecs act as molecular precision tools, recognising the glycan and also the biological environment (or context) these glycans are found in. A 1/5 #glycotime🧵 ⬇️

doi.org/10.1101/2025...

I missed Zeinab's handle: @zeinab-jc.bsky.social.

Shoot, I couldn’t find her handle. Now I got it!

An ultrasensitive and modular platform to detect Siglec ligands and control immune cell function Siglecs are immunomodulatory receptors that regulate immune cell function. A fundamental challenge in studying Siglec-ligand interactions is the low affinity of Siglecs for their ligands. Inspired by ...

Very excited to share some work the Lab has been working on for a while. A new platform to probe Siglec ligands. Led by an incredible PhD student (Zeinab). Comes out of an ongoing fruitful collaboration with the Derda lab. Very open to feedback! www.biorxiv.org/content/10.1...

Contributions by various members of my group: Zeinab, Eddie, @m-joe.bsky.social e.bsky.social, Fahim, and Sam.

The unique molecular recognition features of Siglec-10: structural insights into sialoglycan and antibody interactions Siglec-10 is a sialic acid-binding immunoglobulin-like lectin implicated in immune regulation, yet its molecular basis for ligand recognition and functional role in immune modulation remain poorly def...

New work on the structure of Siglec-10. Great collaboration that was solidified with a visit to Spain last year with fantastic discussions with June Ereno-Orbea and Jesus Jimenez-Barbero. Nice contributions on modeling by @elisafadda.bsky.social.
www.biorxiv.org/content/10.1...

They are quite comfortable. I wear them all the time. Thanks for coming !

Sorry to hear. Hope the rest of your trip goes well.

A little story we put together that I hope can be useful: pubs.rsc.org/en/content/a.... More to come on this work related to this in the future.

Our review on the recent acceleration of research into carbohydrate Sulfation as as an enhancer of Siglec ligands: www.sciencedirect.com/science/arti...

Whoop whoop!

Highly recommended listening to @ghazeskandari.bsky.social if you are interested in immune cells of the brain.

We have a postdoctoral position open to help lead our CD33 project in microglia. Would be grateful if you could spread the word: macauleylab.ca/job-opening-...

Ya. We thought about this a lot to be honest and went back and re-made mutants to be sure. Had some very helpful conversations with Adnan.

Understanding the Glycosylation Pathways Involved in the Biosynthesis of the Sulfated Glycan Ligands for Siglecs Carbohydrate sulfation plays a pivotal role in modulating the strength of Siglec–glycan interactions. Recently, new aspects of Siglec binding to sulfated cell surface carbohydrates have been discovere...

Our latest work on Siglec binding to sulfated glycans. Great help: @khookh.bsky.social, @pengwu.bsky.social ,@raflynn5.bsky.social. Fom 1st PhD graduate of the lab (Jaesoo Jung) and help from Edward, Zeinab, Jhon, Kelli, @tayloregray.bsky.social, and Chris from my lab.
pubs.acs.org/doi/10.1021/...

Enzyme-Sialylation-Controlled Chemical Sulfation of Glycan Epitopes for Decoding the Binding of Siglec Ligands Widely distributed in nature, sulfated glycan epitopes play important roles in diverse pathophysiological processes. However, due to their structural complexity, the preparation of glycan epitopes wit...

With the Wen lab and @siglecdude.bsky.social, we screening a library of sulfated glycan ligands against human Siglecs, and found that sulfation and sialylation patterns serve as important determinants of Siglec binding affinity and specificity #glycotime pubs.acs.org/doi/10.1021/...

Makes sense. Is cell surface glycomics possible to address this?

Ha…I had been thinking about the same issues (posted about this in parallel under Carolyn’s post) but now see Anne’s respond below that is a bit reassuring that this is a very rare event (terminal GlcNAc) under normal healthy conditions.

Good call on @maxcrispin.bsky.social

Indeed, a super intriguing study that I loved reading. Been thinking about this and how rare/'non-self' a terminal GlcNAc is. Hoping to get some input for mass spec community - @cashwood.bsky.social, @nickescott.bsky.social @nmriley.bsky.social @khookh.bsky.social @StacyMalakar (can't your handle)?

Congrats Anne!