Christophe Chapard

How do SMC complexes load onto DNA to get ready for loop extrusion?

@roisnehamelinf.bsky.social & co discovered that Wadjet, an SMC complex involved in bacterial DNA immunity, performs some impressive molecular gymnastics 🤸‍♂️🤸‍♂️🤸‍♂️.

Check out the new paper: www.cell.com/molecular-ce...

#ÉchappéesInattendues 👀 Échappez-vous le temps d'un week-end ! Mars, l'océan, les néandertaliens, l'intelligence... autant de sujets à explorer avec nos scientifiques pour répondre aux questions des petits et des grands.

📍 @citedessciences.bsky.social

👉 echappeesinattendues.cnrs.fr/main-event/o...

Dynamic changes in chromosome and nuclear architecture during maturation of normal and ALS C9orf72 motor neurons We have investigated changes in chromosome conformation, nuclear organization, and transcription during differentiation and maturation of control and mutant motor neurons harboring hexanucleotide expa...

New work from my lab with the Brown lab on how genome folding changes during motor neuron maturation, and how this process goes awry in ALS motor neurons. Led by Dr. Ozgun Uyan.
www.biorxiv.org/content/10.1...

Functional maps of a genomic locus reveal confinement of an enhancer by its target gene Genes are often activated by enhancers located at large genomic distances, and the importance of this positioning is poorly understood. By relocating promoter-reporter constructs into thousands of alt...

Our reporter hopping scaled up to thousands of integrations in a single locus, to produce high-resolution functional maps, with plenty of interesting insights: www.science.org/doi/10.1126/...

Congrats to all !

Synthetic engineering demonstrates that synergy among enhancers involves an increase in transcriptionally productive enhancer-gene contacts Enhancers are non-coding cis-regulatory elements that control the expression of distally located genes in a tissue- and time-specific manner. Recent studies indicate that enhancers can differ in their...

Excited to share the latest work from the lab led by @eharo84.bsky.social, in which we have used synthetic biology to explore the mechanisms by which different types of long-range enhancers ensure robust and precise developmental gene expression

www.biorxiv.org/content/10.1...

RNA Pol II-based regulations of chromosome folding How transcription and cohesin complexes interact to regulate 3D genome organization remains unclear. Chapard et al. dissect their respective contributions and their interplay in shaping the genome arc...

Pleased to see this study out, combining experimental work from our lab at the @cbitoulouse.bsky.social, in particular by @nathaliebastie.bsky.social and @beckof.bsky.social, and from Laura Chaptal and @alengronne.bsky.social at IGH Montpellier
@cnrs.fr @cnrsbiologie.bsky.social

Congrats to all!

Two major events today on our campus!
🚴‍♀️First, the Tour de France is passing right next to us — @cnrsoccitanieest.bsky.social!
🧬Second, our latest paper on the role of G-quadruplexes (G4s) at promoters is now online at @natgenet.nature.com!

Quand l’ARN s’invite dans la réparation de l’ADN Quand notre ADN se casse, nos cellules le réparent avec une haute précision.

Quand l’ARN s’invite dans la réparation de l’ADN ... 🧬

🤝 @lablegube.bsky.social @cbitoulouse.bsky.social

👉 Lire l'article dans @natcellbio.nature.com
buff.ly/CxP2P6N

buff.ly/PY2loYA

Some interesting scientific talks going on!

A CNRS Junior Professorship on RNA Modifications and Advanced… | Centre for Integrative Biology of Toulouse A CNRS Junior Professorship on RNA Modifications and Advanced Epitranscriptomic Technologies offer at CBI-Toulouse Fixed-term (3–6 years), tenure-track (Chaire de Professeur Junior) Application Dead...

www.linkedin.com/posts/centre...

🔥That was #pint25 day 2: WHAT A NIGHT!🔥

Are you ready for the final night? 👓🧠 It's already starting in some countries!

Have a great time and share your photos with us to be featured in the final collage! #pint25

All the info pintofscience.com

💥That was #pint25 day 1 and it was ABSOLUTELY INCREDIBLE! ✨
Ready for day 2?

👓🧠 Teams across 27 countries are already prepping for tonight's science adventures!

📷Share your experience with us to feature in tomorrow's highlight collage!

All festival details at pintofscience.com

Événements à Toulouse Pint of Science provoque LA rencontre entre scientifiques et grand public et lève le voile sur les découvertes et les avancées scientifiques et technologiques. Parce que non, dans un labo ça ne se pas...

On y est ! La science sous toutes ses formes, accessible pour toutes et tous! Hâte d'échanger de manière directe et conviviale avec les spécialistes...

Predicting gene expression from DNA sequence using deep learning models Nature Reviews Genetics - Barbadilla-Martínez et al. review recent progress in deep-learning-based sequence-to-expression models, which predict gene expression levels solely from DNA sequence....

Together with the De Ridder lab www.deridderlab.nl we just published a review on deep learning as a tool for gene expression analysis/ prediction: rdcu.be/elQCc.

Low overlap of transcription factor DNA binding and regulatory targets - Nature A near-complete survey of transcription factor activities in Saccharomyces cerevisiae reveals that most transcription factors have both activator and repressor activities and limited overlap between t...

To the top of the "to-read" list. Looks like a heroic amount of work from the Hahn lab (large-scale ChEC-seq compendium!) www.nature.com/articles/s41...

🎉WOUHOU !
Les tickets pour les soirées #pint25 sont disponibles sur le site, le plus dur sera de faire un choix !
🎟 pintofscience.fr 🎟

🗓 19-21 mai /📍60 villes
Les places dans les bars étant limitées, ne tardez pas à réserver vos places pour vos évènements préférés !

Schematic illustrating the structural changes undergone by MukBEF SMC complex during DNA capture and ingestion.

How do SMC complexes capture DNA? 🧪

Led by Frank Bürmann, Jan Löwe’s & Mark Dillingham’s groups have identified the directional loading mechanism the bacterial SMC complex MukBEF uses to capture & ingest DNA, & found a phage inhibitor to this pathway.

Read more: tinyurl.com/4s8nrrkk

#LMBResearch

YouTube video by Johan Gibcus Rules of Engagement

Earnshaw, Goloborodko, Dekker & Mirny labs are excited to present our latest work, "Rules of engagement for condensins and cohesins guide mitotic chromosome formation" - now accepted!!
www.science.org/doi/10.1126/...
A short clip describing the key results:
www.youtube.com/watch?v=pmvO...

Venez découvrir les intervenants du @cbitoulouse.bsky.social

Why is it so hard to rewrite a genome? Synthetic biologists have the know-how and ambition to retool whole genomes. But the hidden complexity of biological systems continues to surprise them.

Synthetic biologists have the know-how and ambition to retool whole genomes, but hidden complexity continues to surprise them

https://go.nature.com/4gL1QPB

Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) (1/15)
www.biorxiv.org/content/10.1...

DSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts - Nature Communications The critical events in transcription termination of RNA polymerase II (Pol II) remain not fully understood. Here, the authors find that the transcription factor Spt5 enhances the activity of RNA exonu...

Great start of the New Year for Krys from our neighbour lab @oxfordbiochemistry.bsky.social. Have a look at his paper - www.nature.com/articles/s41...

The PNUTS phosphatase complex controls transcription pause release Kelley et al. discover that the PNUTS phosphatase complex plays an essential role in gene transcription by controlling RNA polymerase II pause release. PNUTS achieves this through its TND, which recog...

Very excited that our paper describing a critical role for the PNUTS-PP1 phosphatase complex in transcription pause release is up online! This was a massive team effort with @edimitrova.bsky.social and the rest of the @robklose.bsky.social lab www.cell.com/molecular-ce... A short thread: (1/8)