Milo Imbeni

Join our crazy beautiful project of ExM Atlas !
One more position to fill, this time for a Research Technician at @embl.org in @gautamdey.bsky.social lab.

Come have fun with us !.

Summary figure for study of psilocybin's effect on cortical network connectivity.

New preprint + thread 🧵

#Psychedelics induce the formation of new synapses, but where do they connect?

Our rabies tracing study reveals that #psilocybin shifts connectivity across specific cortical networks.

www.biorxiv.org/content/10.1...

1/12

Glial cells & lipoproteins coordinate lipid & immune signaling to regulate CNS #inflammation. @vinitiwari1.bsky.social & Mikael Simons review the relationship btwn lipid #metabolism & inflammation & how disruptions in this interplay contribute to neurological disorders rupress.org/jem/article/...

Mellon Foundation The Mellon Foundation makes grants to actively unlock the power in the arts and humanities that helps connect us all.

“American Academy of Arts&Sciences reports 96.3% of humanities grads age 23-32 fully employed. Earnings in humanitiess comparable to social/life sciences, job satisfaction levels too. A serious mismatch bw actual employment for hum grads +general perception."
www.mellon.org/voices/human...

Frontiers | Biophysical and computational insights from modeling human cortical pyramidal neurons The human brain’s remarkable computational power enables parallel processing of vast information, integrating sensory inputs, memories, and emotions for rapi...

In our new mini-review “Biophysical and computational insights from modeling human cortical pyramidal neurons” (doi.org/10.3389/fnin...) in
@frontiersin.bsky.social, with Sapir Shapira, @danielay1.bsky.social, Yoni Leibner, Huib Mansvelder, Christiaan de Kock, @mikilon.bsky.social, and Idan Segev,

Side note... I'm only like a hundred pages in, but really enjoying Brandon Sanderson's The Way of Kings.

I have loved most of his books, but DNF'd the second Mistborn book a couple years ago and took a break from him.

Glad I found him again.

Code, visualization apps and data are all freely available!
research.libd.org/spatial_hpc/
bioconductor.org/packages/rel...
github.com/LieberInstit...

Feedback and questions always appreciated!!

"Plug"
Microglia (~20%) physically contract with #EndothelialCell & #Pericyte through gaps b/w adjacent #Astrocyte endfeet

A meticulous analysis of #MICrONS volumetric EM data
1 mm3 🐭visual cortex🤩

@gazz4science.bsky.social @sorengrubb.bsky.social #JCBFM 2024
journals.sagepub.com/doi/10.1177/...

Brain Surfaces of 70 primate species

1
To predict the behaviour of a primate, would you rather base your guess on a closely related species or one with a similar brain shape? We looked at brains & behaviours of 70 species, you’ll be surprised!

🧵Thread on our new preprint with @r3rt0.bsky.social , doi.org/10.1101/2025...

Hey #AAIC25 check out Arash’s presentation today at 8 AM and see some cool whole brain videos of the vasculaturs and plaques plus touchscreen testing

room 106

Evaluating the Efficacy and Risks of Early Lecanemab Treatment in APOE4 Humanized Alzheimer’s Disease Mouse Models

also poster 151

✨Our newest eLife paper is out!✨
👉 elifesciences.org/articles/98662
When retinal cells die, who cleans up?
Non-invasive imaging shows microglia👇 rush to help but don’t recruit neutrophils from the bloodstream. @urneuroscience.bsky.social @cvsuor.bsky.social
@elife.bsky.social #FluorescenceFriday

Now on bioRxiv! Astrocytes in one brain region (cyan) communicate in expansive networks (magenta). Through tool development, tissue clearing, and many hours on our @zeiss-microscopy.bsky.social Z1, we now know these networks are repeatable across mice, plastic, and specific.
doi.org/10.1101/2025...

Ontogeny and function of microglia and nerve-associated macrophages Macrophages are essential immune cells that arise early during embryogenesis and persist as tissue-resident cells into adulthood. This chapter explore…

Microglia and CNS macrophages are multifaceted cells!
We are excited to share our published chapter in Advances in Immunology.
If you are curious about where these cells come from and what roles they play; you can access it here:
lnkd.in/egqqUYQT

Link valid until August 16!
@masslab.bsky.social

Lovely tool, thanks!! I found it a little much to install the whole cellprofiler stacks to then just use the (really comprehensive) morphometrics, so this is great :))
Does it support 3D images? In the preprint the analysis of astrocyte nuclei is run on a max projection?

A chart depicting the track of claims into verified, unchallenged, challenged, partially verified, or mixed. Figure 1: Flow of the study. Claims were first classified in five categories based on literature. 45 unchallenged claims were selected to be experimentally tested and classified either as challenged or as verified. We further classify some unchallenged as consistent or inconsistent depending on their consistency with current knowledge.

But in fact, fly immunity is highly reproducible! 🎊

~80% of claims could be verified. Moreover, some challenged claims just reflect the field advancing its knowledge/tools.

The lesson: if the tools are good and the research largely exempt from direct translation pressures, science works.

3.5/n

Attractors are usually not mechanisms The mathematical objects can not be. And the "attractor models" have not been established as mechanisms in mammals

Attractors are usually not mechanisms - new blog post: open.substack.com/pub/kording/...

'How come I can’t breathe?': Musk's data company draws a backlash in Memphis The company’s turbines — enough to power 280,000 homes — run without emission controls in an area that leads Tennessee in asthma hospitalizations.

whenever i see grok screenshots i remember how it's running on methane-gas generators in a black neighborhood in memphis www.politico.com/news/2025/05...

A panel from a Calvin and Hobbes comic. Calvin's mom, eating dinner, says "Life could be worse, Calvin." Calvin, clearly upset, his mouth huge says "Life could be a lot BETTER too!"

happy birthday to bill watterson who gave me the gift of encapsulating my entire ethical approach in one comic panel.

'By mistaking language as merely a tool that’s a means to an end—little different from a spatula—too many people have lost sight of the fact that understanding language provides the basis of smart thinking. If you offload it to machines, future generations will never develop their own superpowers'

By which I mean: we're not going to understand our own complexity by knowing all about our coding genes. It's all about how those and noncoding parts of the genome, and the molecules that come from them, interact in regulatory networks. /3

Which would mean we go on telling the old ones (blueprints, genes, enzymes, cells as factories etc) that have ever less relevance to the way life really works. That wouldn't be good. We can and must do better. 14/14

OK Mrs/Mr/Ms/Mx Harris. It's good of you to give me an opportunity to explain the physics of the radiation emitted by "highly enriched Uranium". In order to do this properly, I have to explain some of the concepts and units of measurement that are relevant.

I’ll say it since Daniel Stabley won’t: his NeuroImaging Lab didn’t just ride the wave they built it While others were still decoding the manual, they had the Developmental Neurobiology Dept MOSIACing like pros. Ain’t no edge but the cutting edge #MicroscopyMonday www.biorxiv.org/content/10.1...

This was a great sample to check out -- who knew 100M year old amber would be so close to Ethyl Cinnamate in RI? Huge shout out to @brukercorporation.bsky.social for the fleet of lightsheets at @lsfm-na.bsky.social last week!

Spotiflow: accurate and efficient spot detection for fluorescence microscopy with deep stereographic flow regression Nature Methods - Spotiflow uses deep learning for subpixel-accurate spot detection in diverse 2D and 3D images. The improved accuracy offered by Spotiflow enables improved biological insights in...

Spotiflow, our deep learning based spot detection method for microscopy, is now published in @natmethods.nature.com!
Since the pre-print, we have added many features, notably native 3D detection!
@maweigert.bsky.social @gioelelamanno.bsky.social @epfl-brainmind.bsky.social
Paper: rdcu.be/epIB7
(1/N)

Adaptive optics oblique plane microscopy of 100 um thick mouse brain slice with immunofluorescence.

Spending some time at #lsfm25 cleaning up code. It now has distributed GPU gradient-consensus RL decon (@jamesdmanton.bsky.social @andrewgyork.bsky.social) our fast orthogonal deskew, and stitching/fusion to ome-ngff v0.5. Example AO-OPM data on uncleared mouse brain slice github.com/QI2lab/opm-p...

Wow. This is so dangerous. I know the thing is just a plausible phrase generator, but the fact it seems to have been programmed to flatter turns it in effect into a lying seduction machine. Horrified that so many people are rotting their own brains with this this uncanny valley parasocial interface

Reflected Inline Detection in Epi Oblique Plane Microscopy https://www.biorxiv.org/content/10.1101/2025.05.30.657110v1

I had some absolutely incredible papers come online this week, and I need to take a minute to post about each of them. First, let me tell you about the CarboTag probes for imaging plant cell walls! From the Sprakel lab (a short thread) www.nature.com/articles/s41...

‘Half the tree of life’: ecologists’ horror as nature reserves are emptied of insects A new point in history has been reached, entomologists say, as climate-led species’ collapse moves up the food chain even in supposedly protected regions free of pesticides

“One of our very good friends – he now does not have the emotional courage to hang up a sheet to collect moths at night

It is too devastating to see how few there are”
www.theguardian.com/environment/...