Kaisa Pakari

Proud of Kaisa Pakari @kpakari.bsky.social to receive the 2025 Schmeil young researcher award for her #PhD thesis. She established 🐟 models to address disorders of #glycosylation #CDG by advancing #baseediting range for precise genome editing @wittbrodtlab.bsky.social @cos.uni-heidelberg.de

Establishing an auxin-inducible GFP nanobody-based acute protein knockdown system to mimic hypomorphic mutations during early medaka embryogenesis Creating hypomorphic mutations are crucial to study gene function in vivo, especially when null mutations result in (embryonic) lethality. This is especially the case for enzymes involved in glycosyla...

new #preprint: mRNA delivered degron system w/ GFP-nanobody to efficiently reduce GFP-tagged proteins in an inducible manner in 🐟 Case study: reduction of PMM2 (basis for ER-based #glycosylation) causes hypoglycosylation reported from CDG-patients www.biorxiv.org/content/10.1... #CRISPR #RareDisease

Establishing an auxin-inducible GFP nanobody-based acute protein knockdown system to mimic hypomorphic mutations during early medaka embryogenesis Creating hypomorphic mutations are crucial to study gene function in vivo, especially when null mutations result in (embryonic) lethality. This is especially the case for enzymes involved in glycosyla...

Get ready for enzyme limbo & check out our new preprint: We knockdown Pmm2-GFP with a mRNA-delivered degron system in an inducible manner in medaka. Link: www.biorxiv.org/content/10.1101/2025.05.23.655727v1
Thanks to @thomthum.bsky.social, @wittbrodtlab.bsky.social & colleagues
#greennomore #medaka

Calling all #MedakaPI researchers! Join us in Heidelberg, Germany from July 22-24, 2025 for our collaborative meeting! Register now & take advantage of early bird rates until April 15th! meetmedaka.com/registration/ 🐟 #MedakaResearch #ModelOrganisms #ScienceConference #FishModels #Heidelberg2025