Exciting Instruments

⏰ What if your experimental workflow went from weeks to minutes? @shenk0128.bsky.social shares how solution-based measurements complement TIRF:

✓ Faster results
✓ Fewer artefacts
✓ No tethering needed
✓ Proteins stay in their native, comfortable state

#singlemolecule #TIRF #proteinstructure

In this month's newsletter: a big RNA breakthrough, the power of combining structural data with dynamics, getting super fast answers to your biological questions, and some fantastic PhD opportunities. Register - fwfh6.share-eu1.hsforms.com/2tB-S9AikSdG... #rna #structuralbiology #singlemolecule

👋 Calling all Prague-based structural biologists and single-molecule enthusiasts! You are invited to listen and learn from @timcraggs.bsky.social on 9 Dec at the J. Heyrovský Institute of Physical Chemistry - www.jh-inst.cas.cz/seminar/tim-... #singlemolecule #structuralbiology #smfret #fcs

A DNA Scaffold Approach Facilitates 5′ Labeling of the SARS‐CoV‐2 RNA Pseudoknot for Single‐Molecule Förster Resonance Energy Transfer Investigation DNA scaffold-based labeling strategy is introduced that enables single-molecule Förster resonance energy transfer (smFRET) studies of the SARS-CoV-2 frameshifting pseudoknot. The smFRET measurements ...

Curious about new routes for labelling often complicated RNAs for single-molecule FRET studies? You'll want to check out Graham et al.'s recently published work in ChemPhysChem (congratulations 🥳) - buff.ly/SkaruHO
#rna #rnaresearch #virology #singlemolecule #smfret #nucleicacids

YouTube video by Exciting Instruments Mapping the global conformation of the Rag GTPase heterodimer with smFRET | Exciting Seminars

🔎 How does in-solution smFRET reveal the dynamic structure–function links in complex proteins?
Watch Prof. @shenk0128.bsky.social's seminar using the EI-FLEX to probe Rag GTPase conformations. Q&A covers labelling, HMMs & why solution-based smFRET matters.
youtu.be/2AfskEVyRCI #smfret #biophysics

Two hours to go! Join @shenk0128.bsky.social and @timcraggs.bsky.social as they dive into Rag GTPase regulation, #proteindynamics, and cutting-edge #singlemolecule science. Can’t make it live? Register to get the recording - us06web.zoom.us/meeting/regi...
#biophysics #lifescience #biophysics

🚨 Happening this Thursday! @timcraggs.bsky.social and @shenk0128.bsky.social discussing:

→ cutting-edge single-molecule science
→ insights into the mechanism and regulation of the Rag GTPase heterodimer

Register here: buff.ly/GcdLpHI #seminar #proteins #singlemolecule #biophysics #biochemistry

Mechanistic insights into the activity of SARS-CoV-2 RNA polymerase inhibitors using single-molecule FRET Abstract. The COVID-19 pandemic, caused by the SARS-CoV-2 virus, has resulted in significant global mortality, with over 7 million cases reported. Despite

Professor Robb says: "Our EI-FLEX platform has been central to understanding the molecular details of viral replication and drug action. It’s quickly become a core instrument across many aspects of our virology research." Read her paper here - doi.org/10.1093/nar/... #smFRET #drugdevelopment

⚡ What do you get when you combine a meaningful mission, innovative solutions, and an epic group of people? Something extremely Exciting 😉 We're looking for brilliant people to fill these roles & more > excitinginstruments.com/careers/

#lifesciencejobs #startupjobs #sheffieldjobs #hiring #scaleup

Join Kuang and @timcraggs.bsky.social on 20th November to discuss his findings in more detail as part of our next Exciting Seminar - 'Mapping the global conformation of the Rag GTPase heterodimer with single-molecule FRET' > us06web.zoom.us/meeting/regi... 🧵 4/4

#structuralbiology #biophysics

@shenk0128.bsky.social's recent work exemplifies this beautifully. Starting from atomic structures, he and Doxsey used smFRET to determine the relative populations of different states present in dynamic equilibrium under different conditions to unpick the detailed mechanism of their system. 🧵 3/4

For many proteins, it has become evident that function is not defined by a single static structure. Instead, it is the dynamic interconversion between multiple conformations that enables and regulates their biological activity. 🧵 2/4

🤔 Who is familiar with the traditional structure–function paradigm that a protein’s atomic structure determines its function? 🧵 1/4

🇨🇭 Meet Roman at #PSDI2025 (poster PO21) to see how our tools reveal drug-induced conformational changes, bringing static structures to life. Then 🇦🇹 join him & Tim at the 8th Austrian Cryo-EM Symposium. DM us to connect! #cryoem #drugdiscovery #structuralbiology

📢 Our Exciting Seminars are back! Join @timcraggs.bsky.social & @shenk0128.bsky.social on Nov 20 for 'Mapping the global conformation of the Rag GTPase heterodimer with single-molecule FRET' > us06web.zoom.us/meeting/regi...

#biophysics #lifescienceevent #molecularmedicine #biochemistry #smfret

Erin is currently hiring for a fully funded PhD position to work collaboratively between her lab, Tim Craggs and Exciting Instruments. Find out more about her work here > cuttslab.uk

✨ Watch Dr Erin Cutts use the EI-FLEX and imagine the discoveries you could make yourself! We’re offering labs and core facilities a 2 month EI-FLEX trial at no cost. Run your own samples and see the data for yourself. Apply by Dec 15 → bit.ly/4ojRDhF
#singlemolecule #biophysics #lifescience

The transitional kinetics between open and closed Rep structures can be tuned by salt via two intermediate states DNA helicases undergo conformational changes; however, their structural dynamics are poorly understood. Here, we study single molecules of superfamily 1A DNA helicase Rep, which undergo conformational...

What happens when fluorescence detection reaches nanosecond precision? Recent work in time-resolved single-molecule FRET from Howard et al. using the EI-FLEX reveals fast, transient conformations and connects photon-level observables with structural and dynamic models - biorxiv.org/content/10.1...

October roundup Hi there, I hope you're doing well. I'm excited to say our Exciting Seminars will be back with a bang in November! Join me and Kuang Shen from UMass Chan Medical School as we explore his latest smFRET...

🚀 Exciting news! Submit a short proposal on how you'd use the EI-FLEX in your lab for a chance at a 2-month, no-obligation trial! Plus: see smFRET in action, upcoming seminars and more 👉 www.linkedin.com/pulse/octobe...
#biophysics #lifescience #moleculardynamics #singlemolecule

How can our stable, compact, and easy-to-use single-molecule tools bring your static models to life? Check out the poster our Senior Application Scientist shared at last week’s Advances in Structural Analysis of Biomolecules conference👇 #structuralbiology #singlemolecule #molecularbiology

Cut prep time, skip immobilisation, and get real-time molecular insights with Fluorescence Correlation Spectroscopy (FCS).

✓ In-solution, label–mix–measure
✓ Setup in a day
✓ First results in minutes

Spend less time prepping, more time discovering. Find out more > bit.ly/47pRqm1

#fcs #kinetics

Average doesn't really exist... so why measure it?

Our compact and easy-to-use instrument has been designed to help researchers access dynamic single-molecule insights from their own lab benchtops.

Find out more about the EI-FLEX and book a demo bit.ly/3KZK3Ku

#singlemolecule #smfret #fcs

🚀 Join our mission to democratise single-molecule analysis! We’re hiring:

Scientific Creative Writer

Product Manager – Assay Kits

Field Application Scientists

Apply now 👉 excitinginstruments.com/careers?utm_...

#sheffieldjobs #startupjobs #lifescience #hiring

Great chatting dynamic single-molecule data with brilliant scientists at the Crick yesterday! 🇩🇪 Next stop: Advances in Structural Analysis of Biomolecules at Charité Berlin, Oct 23–24. Don’t miss our Senior Application Scientist
Mahmoud’s poster - P017! #structuralbiology #chembiochem #chemphyschem

Join Tim Craggs, Paul Wheeler & the single-molecule community at The Francis Crick Institute, London - this Monday! ⚡ Dive into the latest single-molecule research, techniques & discoveries. Free to attend - reserve your spot: www.eventbrite.co.uk/e/single-mol...
#biophysics #molecularbiology

Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study - Nature Methods A multi-laboratory study finds that single-molecule FRET is a reproducible and reliable approach for determining accurate distances in dye-labeled DNA duplexes.

Can smFRET deliver reproducible, absolute distance measurements?
Yes! Check out Hellenkamp et al.'s (2018) paper to learn more > www.nature.com/articles/s41...

#singlemolecule #smfret #biophyics #biochemistry

🚀 Since spinning out in 2021, our journey has been an Exciting one! Thrilled to see our CEO & founder @timcraggs.bsky.social named Entrepreneur of the Month by Insider Magazine. Read Tim’s comment below and the article here 👉 flickread.com/edition/html...

#entrepreneur #singlemolecule #sheffield

🚀 Our 'professor in a box', a quick-watch smFRET explainer video, a very exciting Chair appointment and more... see what we've been up to in September in the latest edition of our newsletter - buff.ly/EDA6U5s

#structuralbiology #moleculardynamics #singlemolecule #smfret #fcs #deeptech #startups

“Absolutely gorgeous!" That's how Edwin Antony describes the data from our EI-FLEX in his Exciting Seminar, where he discusses how DNA context regulates the myriad roles of RPA. Watch the seminar in full to learn more: youtu.be/r0yowVKdXUI

#DNA #singlemolecule #molecularbiology #structuralbiology

🚀 Here comes the next big step in our Exciting journey! Thrilled to announce the appointment of Jonathan Rigby as the Chairman of our Board. Find out more about what it means for Exciting Instruments in the press release below.
#innovation #startups #biophysics #Sheffield #pressrelease