Alex Kiryuskin

What if we could deliver guide RNAs by Agroinfiltration and Cas9 by tagging with third alpha helix domain? 🤔

What if the biological process was described by famous filmmaker?
#ubiquitin #biology #science

#auxin
Fantastic work! Must read!

Roots employ several classes of hormones including rhizocrine signals that spend time outside of plant tissues in the rhizosphere. A schematic diagram illustrating (A) a longitudinal cross-section of a root growing in soil, and (B–E) examples of the contrasting modes of action for different hormone classes (denoted as yellow circles) involving (B) autocrine signalling (i.e. acting within the source cell), (C) paracrine signalling (i.e. acting close to the source cell), (D) endocrine signalling (i.e. acting in other organs after long-distance transport), and (E) rhizocrine signalling, where this class of hormone moves outside its root source via the soil (denoted by the blue arrow), returning to the root in response to a soil stress (denoted by the red arrow), triggering an adaptive response, denoted by the green arrow and receptor binding of the hormone signal.

🌱📡⚠️ DARWIN REVIEW 🌱📡⚠️

'Root RADAR: how ‘rhizocrine’ signals allow roots to detect and respond to their soil environment and stresses' – Bipin K Pandey et al. doi.org/10.1093/jxb/...

#PlantScience 🧪

Scientists map part of a mouse's brain that's so complex it looks like a galaxy Thanks to a mouse watching clips from “The Matrix,” scientists have created the largest functional map of a brain to date.

Thanks to a mouse watching clips from "The Matrix," scientists have created the largest functional map of a brain to date.

A space for time. Exploring temporal regulation of plant development across spatial scales Developmental timing in plants is tightly regulated at the whole-organism scale, from germination to flowering. Less is known about when and how local transitions are temporally regulated. Not every ...

Our lab's first review article written with @yadhusankar.bsky.social and @vijayalakshmi.bsky.social is out now in The Plant Journal! 🎉🌱

We discuss temporal regulation of (plant) development. When and how do plants control organ and cell identity progression?

onlinelibrary.wiley.com/doi/10.1111/...

#AlphaFold #ProteinStructure vs metal sponge scrubber for dishes, find the difference :)

To do volcano plot, or to drink (coffee) volcano pot - that is the question :)

#VolcanoPlot vs volcano pot
📊 vs ☕️🍰

New Article: "Developmental innovation of inferior ovaries and flower sex orchestrated by KNOX1 in cucurbits" rdcu.be/ef78H

Role of a KNOX1 TF in stalling receptacle growth in cucumber, leading to bisexual flowers with superior ovaries like those in tomatoes.

Thank you, cause the link hasn't attached in my post

I'm not alone in joking about R2D2 #auxin sensor :)

P.S. Thank you to @robertboisivonlab.bsky.social for cool auxin perception infographics (boisivonlab.ceitec.cz/handouts/) and to @aribidopsis.bsky.social for the blog post 'R2D2: Auxin marker from Star Wars' (www.aribidopsis.com/2017/12/r2d2...)

Cool! One more cucumber mutant, 𝙙𝙚𝙡𝙖𝙮𝙚𝙙 𝙜𝙧𝙤𝙬𝙩𝙝 (𝙙𝙡), with short root system.

CsIREH1 phosphorylation regulates DELLA protein affecting plant height in cucumber (Cucumis sativus).

Zhao et al.
in @newphyt.bsky.social
👉https://doi.org/10.1111/nph.20309

CsTIE1-CsAGL16 module key for cucumber lateral branch & drought tolerance. #CucumberBreeding @OxfordJournals
Details: doi.org/10.1093/hr/u...

Look here for this problem:
👉https://x.com/Alex_Kiryushkin/status/1871980232468832481?t=83EXIMZ9M4qrhLtX03Xdeg&s=19

👉https://x.com/LaurensPauwels/status/1638842429347332096?t=ZmL9kMWgZ5xnZ_GUK5d0cg&s=19

👉https://x.com/Alex_Kiryushkin/status/1640356276868726784?t=LVBUrDTPtLM3XTG8zMvjPQ&s=19

What if we could stabilize expression of the RUBY reporter by replacement of P2A linkers just with long GS-linkers❓️ 🤔

The similar, three-enzyme system is well used in base editing: UGI-nCas9-nucleoside deaminase.

Tag for @atinygreencell.bsky.social, @laurenspauwels.bsky.social

Do you want to know how to live-image internal floral organs with confocal microscope for up to 2 weeks?
Check out our protocol:
bio-protocol.org/en/bpdetail?...
#Arabidopsis #Flower

Interestig study from potato research area

A diploid potato line, Jan, and knockout mini-Jan mutants.

Volume 196 Issue 4 | Plant Physiology | Oxford Academic Plant Physiology is an international journal devoted to physiology, biochemistry, cellular and molecular biology, genetics, biophysics, and environmental biology of plants

Plant Physiology regularly publishes focus issues that dive deep into key areas of plant science. From plant stress responses to metabolic regulation, these issues bring together cutting-edge research on critical topics.

📖Read the latest focus issue at academic.oup.com/plphys/issue.... 🌿

A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network in Planta An improved synthetic reporter monitors cytokinin signaling in planta.

Thanks. It is the TCSn version (doi.org/10.1104/pp.1...). The holes are absence of signal in developing lateral root primordia and nearby RAM initials.

@plantphys.bsky.social's Focus Issue on Root Development.
academic.oup.com/plphys/issue/196

Genome editing in almond using hairy root transformation system - Plant Cell, Tissue and Organ Culture (PCTOC) Woody plant species are often challenging to transform, which complicates gene function studies using molecular biology techniques. To develop an effective gene editing tool for almond (Prunus dulcis,...

Our new publication in PCTOC:
CRISPR-induced mutagenesis in Almond hybrids by hairy root transformation.
doi.org/10.1007/s112...
#CEITEC_Brno #TANGENC #INTERCOST #RoxyCOST