Zhixing Chen

🌑Goodbye bleaching, hello brightness! ☀️

We're thrilled to introduce two new Halotag ligands featuring @zhixingchen2.bsky.social fantastic photoresistant BD-dyes!

✅ Highly photoresistant, no photoblueing
✅ Outstanding brightness
✅ Cell permeable
✅ Outperform best in class fluorophores

Check it out at PNAS today. Thank you Alexandre for the collaboration!!

PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by
Jing Ling, Yitong Liu, and all the collaborators!

Available @spirochrome.com

Fast, Bright and Reversible Rhodamine Tags for Live-Cell Imaging We present Rho-tag and SiR-tag, engineered protein tags derived from bacterial multidrug-resistance proteins that bind unsubstituted (silicon-) rhodamines with nanomolar affinity, enabling fast, rever...

Teaching an old bacterial protein new tricks: Fast, bright and reversible rhodamine tags for live-cell imaging:

www.biorxiv.org/content/10.1...

Congratulations to Julian Kompa and the entire team.

SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.

Our groundbreaking Dark Sectioning technique is now published in Nature Methods! 🎉 It supercharges existing imaging systems:
🔬 Widefield → Confocal
🔬 Single-photon → Multiphoton
🔬 2D-SIM → 3D-SIM
Now integrated in Airy PolarSIM and #FlexSIM!
doi.org/10.1038/s415...
#Microscopy #Fluorescence

Looking for a postdoc - Shi Lab The Shi lab at Rutgers-New Brunswick is seeking a postdoctoral researcher to study biomolecular condensates and/or mechanosensitive membrane proteins. To see more information and apply, please go to t...

We are looking for a postdoc to study biomolecular condensates and/or mechanosensitive membrane proteins. Please help spread the news 🙏
sites.rutgers.edu/shi-lab/look...

Thank you Shinya!!!

A palette of bridged bicycle-strengthened fluorophores - Nature Methods A suite of bridged rhodamine dyes (BriDyes) offers excellent brightness, solubility, photostability, and tunable cell permeability along with resistance to photoblueing, making them exceptional all-pu...

Here come our new palette of fluorescent dyes. The BD dyes endeaves to balance glowy brightness, robust photostability, and biocompatibility. Let's boost 4D dynamic super-resolution imaging!!!
www.nature.com/articles/s41...
@spirochrome.com

Excited to share my first post on Bluesky: I’ve recently been appointed as a Professor at Goethe University Frankfurt! 🎉

The Stephan Lab will use advanced bioimaging 🔬🧫 to explore how organelles collaborate to maintain subcellular architecture and metabolism.

A comprehensive review on covalent chemical strategies for protein self-labeling from @kjohnsson.bsky.social's lab: HaloTag, SNAP-tag et al. www.annualreviews.org/content/jour...

Happy to share DeepCristae😀, a CNN for mitochondria restoration, now in @commsbio.bsky.social y.social

rdcu.be/ebrAQ

S.Papereux, @ludovicleconte.bsky.social, @zhixingchen2.bsky.social, J.Salamero, C.Kervrann, A.Badoual
@the.3i.social @institutcurie.bsky.social #inria @france-bioimaging.bsky.social

Thank you Shinya for the invitation! Very much looking forward to this trip!!!

🔔🧪 I'm deeply saddened by the passing of Michael Sheetz, a true pioneer in #mechanobiology.
I'll always cherish our lovely times in NYC, Singapore, Zurich, and Sydney. My heartfelt condolences to his family and friends in this difficult time.
So long, Mike 🥺😔

Oh magazine maker, let us transfer our pretty papers to her, instead of nature communications.

Thank you for sharing this touching piece!

How and when to measure mitochondrial inner membrane potentials The scientific literature on mitochondria has increased significantly over the years due to findings that these organelles have widespread roles in the onset and progression of pathological conditions such as metabolic disorders, neurodegenerative and cardiovascular diseases, inflammation, and cancer. Researchers have extensively explored how mitochondrial properties and functions are modified in different models, often using fluorescent inner mitochondrial membrane potential (ΔΨm) probes to assess functional mitochondrial aspects such as protonmotive force and oxidative phosphorylation.

Mitochondria vibes only! Want to measure the inner membrane potential but unsure how or when? This review breaks it down! From protocols to pitfalls, get the tools you need to decode cellular powerhouses. ow.ly/Ejj050UsK7Y

I always tell this "discouraging" story to the grad students on my class...

First post on Bluesky!✨
(1/7) Far-red dopamine sensor is coming! Excited to introduce our chemigenetic DA sensor, HaloDA1.0! #Dopamine #FarRedSensors, #GRAB www.biorxiv.org/content/10.1...

Thank you for choosing PK Mito!

Thank you Christophe for the promotion!!

How proximal is proximity labeling❓ Using DNA nanorulers we found that TurboID/BioID are largely contact labeling, while APEX2 also bears a major contact labeling pathway in parallel with diffusive labeling.
www.biorxiv.org/content/10.1...

Special thanks to @aliceyting.bsky.social for discussions

Thank you Christophe! Actually I would say the live cell STED dye PK Mito Orange is more visually impressive:)

Starting the week strong! Cheering myself up for the next challenges with 15 min of axonal growth cone dynamic madness. F-actin (cyan) and membrane (magenta) show their dance. One image taken every 10 s.🧠🔬
#MicroscopyMonday #LiveImaging #Neuroscience #Microscopy

I feel the science-to-nonsense ratio is much better on this site:

Enjoy these jittery mitochondria sifting through axons of patient-derived motor neurons thanks to the pkMito probe from @spirochrome.com

#neuroscience #science #microscopy #bioimaging #mitochondria