Triangle-beam interference structured illumination microscopy - Nature Photonics Triangle-beam interference structured illumination microscopy leverages radially polarized beams to generate two-dimensional lattice illumination patterns. The technique enables a temporal resolution ...

Nat. Photonics🔬 Meet 3I-SIM!
Instead of stripes, we use 3-beam to generate 2D hex pattern for simultaneous 2D SIM modulation, with >100,000 frames, 13 h neuronal cell movies, ER–lipid-droplet kiss-n-run and millisecond tango. All hard&software open.
doi.org/10.1038/s415... @pku1898.bsky.social

High-fidelity tissue super-resolution imaging achieved with confocal2 spinning-disk image scanning microscopy - Light: Science & Applications C²SD-ISM integrates an SD and a DMD to form a dual confocal configuration, enabling deep-tissue super-resolution imaging with DPA-PR reconstruction.

C²SD-ISM combines physical defocus rejection (spinning disk) with adaptive algorithm (DPA-PR), breaking depth limits in tissue super-resolution (↑180 μm), enhances fidelity (92% linear correlation), and supports high-throughput imaging. doi.org/10.1038/s413... Github: github.com/Chauncey-Leu....

Ultra-stable, ultra bright, congratulations!

mScarlet3-H (aka mYongHong) is on the cover of Nature Methods! All plasmids are available for free from WeKwikGene wekwikgene.wllsb.edu.cn/publications...

Congratulations to Suyi Zhong, Wei Ren, Ziyi Yang, and Hongyu Wang!

If you have a dual channel, it can certainly be implemented on either one, or both.😁

Oh no, it does not need any hardware modification. It is like any Fiji plugin - it removes the fluorescence background through algorithm.😁

Congratulations Stephan!🎉🎉🎉

I am very glad to see that our PolarSIM helps the leading scientists to observe richer dynamics and get more discovery! Congratulations to Zhixing team for this brand new palette of fluorescent dyes named after BeiDa! www.nature.com/articles/s41... @pku1898.bsky.social @zhixingchen2.bsky.social

A palette of bridged bicycle-strengthened fluorophores - Nature Methods A suite of bridged rhodamine dyes (BriDyes) offers excellent brightness, solubility, photostability, and tunable cell permeability along with resistance to photoblueing, making them exceptional all-pu...

Here come our new palette of fluorescent dyes. The BD dyes endeaves to balance glowy brightness, robust photostability, and biocompatibility. Let's boost 4D dynamic super-resolution imaging!!!
www.nature.com/articles/s41...
@spirochrome.com

I am very glad to see that our PolarSIM helps the leading scientists to observe richer dynamics and get more discovery! Congratulations to Zhixing team for this brand new palette of fluorescent dyes named after BeiDa! www.nature.com/articles/s41... @pku1898.bsky.social @zhixingchen2.bsky.social

I am very glad to see that our PolarSIM helps the leading scientists to observe richer dynamics and get more discovery! Congratulations to Zhixing team for this brand new palette of fluorescent dyes named after BeiDa! @pku1898.bsky.social @zhixingchen2.bsky.social

I am very glad to see that our PolarSIM helps the leading scientists to observe richer dynamics and get more discovery! Congratulations to Zhixing team for this brand new palette of fluorescent dyes named after BeiDa! @pku1898.bsky.social @zhixingchen2.bsky.social

Thrilled to introduce our Cover review: High-spatiotemporal-resolution structured illumination microscopy: principles, instrumentation, and applications dx.doi.org/10.3788/PI.2...

Beautiful mitochondria!👍👍👍

Hi Rita, nice to see you at Bluesky! 😁😁😁

Unlock the secrets of cellular dynamics with high-spatiotemporal-resolution structured illumination microscopy! Our latest review explores the principles, instrumentation, and applications of SIM&ISM in live-cell imaging. #Microscopy #SuperResolution #CellBiology dx.doi.org/10.3788/PI.2...

Seeing Deeper, Clearer than Ever!🔬Prof. Xi Peng’s team from #PKU College of Future Technology introduces Dark Sectioning in Nature Methods—a breakthrough for fluorescence microscopy!

A peek into the future of imaging:
www.nature.com/articles/s41...

#PKUResearch #AcademicSky @natmethods.nature.com

Dark-based optical sectioning assists background removal in fluorescence microscopy - Nature Methods The Dark sectioning algorithm removes the background and provides single-frame optical sectioning in fluorescence microscopy. It offers improved quantitative analysis and deep-tissue segmentation accu...

Dark matter, Dark channel matters! 😀
Link to our paper and commentary: www.nature.com/articles/s41... www.nature.com/articles/s41...

For a quick summary of the paper, read the Research Briefing here:
www.nature.com/articles/s41...

Thank you! 😁😁😁

Nice work! We integrated this dark-sectioning method into our FlexSIM pipeline (github.com/esoubies/Fle...) as an additional option for handling out-of-focus signal. It works remarkably well!

In particular, it handles out-of-focus regions more effectively than traditional OTF attenuation.

Our groundbreaking Dark Sectioning technique is now published in Nature Methods! 🎉 It supercharges existing imaging systems:
🔬 Widefield → Confocal
🔬 Single-photon → Multiphoton
🔬 2D-SIM → 3D-SIM
Now integrated in Airy PolarSIM and #FlexSIM!
doi.org/10.1038/s415...
#Microscopy #Fluorescence