The collection of papers emphasises the interconnection between microscopy and microbiology, advancing understanding of infectious diseases.

View the issue here: onlinelibrary.wiley.com/toc/13652818...

📣 The Journal of Microscopy is pleased to announce the publication of a new special issue, Microscopy and Infectious Diseases!

The special issue was guest edited by Leandro Lemgruber (University of Glasgow, UK) and Mariana De Niz (Northwestern University, USA).

3SD: Rotational symmetry single‐shot denoising in fluorescence microscopy Image noise is a fundamental problem in fluorescence microscopy analysis, especially in live cell imaging applications where the number of detected photons is limited due to low power of excitation l...

"3SD: Rotational symmetry single-shot denoising in fluorescence microscopy" by Tijmen H. de Wolf, Pleun Engbers, Justine Perrin, Julie Nonnekens and Ihor Smal is free-to-view here: doi.org/10.1111/jmi....

... algorithm makes use of the symmetry often present in biological structures. By selecting a single example of a symmetric biological structure from a dataset, their denoiser algorithm learns how to denoise the entire dataset effectively.

🔬 In this new Open Access paper, the authors propose a new method that does not require a lot of computing power and works even for small datasets that are more typically seen in life sciences. To maintain competitive performance with respect to state-of-the-art methods, their denoising...

Single‐molecule validation and optimised protocols for the use of secondary nanobodies in multiplexed immunoassays Antibodies have long been nature's solution to targeting molecules of interest. In human health, they are created by the body to locate and bind protein that shouldn't be there, such as those produce....

"Single-molecule validation and optimised protocols for the use of secondary nanobodies in multiplexed immunoassays" by Rebecca Saleeb, Judi O'Shaughnessy, Ryan Ferguson, Candace T. Adams and Mathew H. Horrocks is free-to-view here: tinyurl.com/yk3suh7w

Using these tools, they analysed the binding properties of commercially available secondary nanobodies and outline optimised protocols for their robust use.

🔬 In this #OpenAccess paper, the authors propose a set of experiments spanning total internal reflection fluorescence and confocal microscopies that can be used to validate secondary nanobody binding, specificity, and their propensity for mis-targeted binding in multiplex assays.

Call for Papers - NanoInBio 2026

Guest editors: Mingdong Dong and Kislon Voitchovsky

Deadline for submissions: 31 August 2026

www.rms.org.uk/resources/jo...

NanoInBio 2026 will support four broad themes, each underpinned or directly focused on microscopies: Bio- & Nanotechnology for health & environment, Bio- & Nanomaterials from labs to medical applications, The bio & non-bio interface, and Intrumentations for materials & life sciences.

NanoInBio 2026 will celebrate the ten-year anniversary of this biennial conference, and will be larger than the previous editions, including scores of internationally renowned speakers and their graduate students.

📣 The Journal is pleased to announce a new special issue dedicated to the international conference NanoInBio 2026.

This issue will gather under one umbrella contributions from the papers presented at the NanoInBio 2026 international conference that will be held 30 May – 5 June 2026 in Guadeloupe.

Determining bismuth content in GaAsBi alloys by energy‐dispersive X‐ray spectroscopy: A case study with multiple sets of k*‐factors for analytical transmission electron microscopy Measuring the bismuth (Bi) content of ternary gallium arsenide bismuthide (GaAsBi) alloys is important because it sensitively influences their bandgap, and Bi is known to segregate vertically to the ...

"Determining bismuth content in GaAsBi alloys by energy-dispersive X-ray spectroscopy: A case study with multiple sets of k*-factors for analytical transmission electron microscopy" by Thomas Walther is free-to-view here: doi.org/10.1111/jmi....

...vertically to the surface and sometimes also laterally during growth, so elemental distribution maps need to be quantified. A suitable method is mapping of characteristic X-rays based on energy-dispersive X-ray spectroscopy (EDXS) in a scanning transmission electron microscope (STEM).

🔬In this new paper, the author describes how measuring the bismuth (Bi) content of ternary gallium arsenide bismuthide (GaAsBi) alloys is important because it sensitively influences their bandgap, and Bi is known to segregate...

"Microfluidic live-cell imaging of Aspergillus fumigatus and Candida albicans hyphal growth treated with AmBisome and Caspofungin" by D. D. Thomson, R. Inman, S. Nye and E. M. Bignell is free-to-view here: doi.org/10.1111/jmi....

The authors describe the cell death and/or adaptive growth responses via live-cell microscopy to understand the morphological and cellular responses over time.

🔬 This new open access paper shows how the invasive filamentous form of two major human fungal pathogens respond to two frontline clinical antifungal drugs with real time microscopy.

Call for Papers - Taking the Stage: The Future of Microscopy

This special issue will celebrate the contributions of Early Career Researchers in the field of microscopy and we look forward to reading your submissions!

Deadline: 27th March 2026
More info: tinyurl.com/2s3t7utb

📣 Call for papers: Taking the Stage: The Future of Microscopy

The Journal has launched an open call for papers for an exciting new special issue which will feature submissions by Early Career Researchers. The issue will be guest edited by Katherine Paine, Myfanwy Adams and Colum O'Leary.

Call for Papers - 9th International BioBrillouin Conference

Special issue in @jofmicroscopy.bsky.social featuring papers from contributors to the 9th International BioBrillouin Conference and open submissions on the topic of Brillouin microscopy/spectroscopy of biological and bio-relevant matter. Deadline: 01.06.2026
www.rms.org.uk/resources/jo...

MAM‐UNet: A multiple attention mechanism UNet for the eutectic segmentation of superalloys The eutectic area fraction is a critical indicator of metallographic properties and directly influences the mechanical properties of superalloys. Rapid and accurate detection of eutectic regions is e...

"MAM-UNet: A multiple attention mechanism UNet for the eutectic segmentation of superalloys" by Meng'ao Li, Zhihao Yue, Haotian Gao, Bing Yang, Erren Yao and Qing Wang is available to view here: doi.org/10.1111/jmi....

The proposed approach offers a reliable tool for automated eutectic-region extraction, providing essential support for subsequent eutectic content evaluation in metallographic analysis.

🔬This new study presents a MAM-UNet–based segmentation method for accurately identifying eutectic regions in metallographic images. By incorporating multi-attention modules to enhance feature representation, the model effectively delineates complex eutectic structures.

Call for Papers - 9th International BioBrillouin Conference

Guest Editors:
- Kareem Elsayad (Medical University of Vienna)
- Stephanie Möllmert (Max Planck institute for the Science of Light)
- Maddy Parsons (King's College London)
- Robert Prevedel (European Molecular Biology Laboratory)

tinyurl.com/49xcmp8p

In addition we welcome open submissions on the topic of Brillouin microscopy/spectroscopy of biological and bio-relevant matter.

Deadline for submissions: 1 June 2026

📣 Open call for papers: 9th International BioBrillouin Conference!

The Journal of Microscopy is pleased to announce a new special issue featuring papers from contributors to 9th International BioBrillouin Conference, hosted by the International BioBrillouin Society.

"Application of Noise2Inverse and adaptation (Noise2Phase) to single-mask x-ray phase contrast micro-computed tomography" by Khushal Shah et al is free-to-view here: doi.org/10.1111/jmi....

However, the mask lowers x-ray flux, requiring longer exposures. The authors show that this requirement can be relaxed by integrating denoising into the processing pipeline. An adaptation, taking advantage of the imaging system, is introduced, not relying on splitting the CT dataset.

🔬 In this new paper, the authors state that single-mask edge illumination x-ray phase contrast micro-CT improves 3D imaging of weakly attenuating samples. The beam is modulated into 'beamlets' aligned to pixel boundaries by the mask, so their refraction-induced deviations can be used to form images.