Diagram showing four configurations of a tip interacting with a sample surface in near-field microscopy, with corresponding heat maps of O4 near-field amplitude and electric field enhancement distributions.

🔬 This new open access paper by W. Pfeiffer, N. S. Mueller, R. Hillenbrand, I. Niehues & P. Kusch, presents the realisation of correlative TEPL/TAPL and s-SNOM measurements within a single side-illuminated near-field optical microscope.

Free to view here: doi.org/10.1111/jmi....

📣There are >100 UK Institutions who are part of a deal between Wiley and Jisc which allows researchers free #OpenAccess publication in the Journal of Microscopy at no direct cost. The cost of publishing is covered under the terms of the agreement.

Check your eligibility here > bit.ly/4g2KYU1

"Crossing scales and eras: Correlative multimodal microscopy heritage studies" by Charles Wood, George Deakin, Atousa Moayedi and Jovana Radulovic is free-to-view here > doi.org/10.1111/jmi....

and advances of key techniques, such as electron, X-ray, optical, and probe microscopies. The review presents practical applications through case studies on materials that include wood, pigments, ceramics, metals, and textiles.

🔬This new Open Access paper provides a comprehensive review of the evolution, current applications, and future trajectory of CoMic within the field of heritage science. The authors present a historical overview of microscopy in heritage studies and detail the principles...

Special Issue: Festschrift for Professor Paul Walther: Journal of Microscopy: Vol 299, No 3 The <em>Journal of Microscopy</em> is the oldest journal dedicated to the science of microscopy, publishing the latest advances in microscopy techniques and novel areas of application.

The September 2025 special issue was edited by Clarissa Read, Ulm University, Germany, and Ulla Neumann, Max Planck Institute for Plant Breeding Research, Germany.

tinyurl.com/4j4pvyvz

📣 The Journal has published a special issue, honouring the career of Professor Paul Walther and his legacy in the field of electron microscopy.

In March 2024, after 46 years, he retired from Ulm University, Germany, where he led the Central Facility for Electron Microscopy.

"Leveraging modified ex situ tomography data for segmentation of in situ synchrotron X-ray computed tomography" by Tristan Manchester et al is open access and free-to-view here: doi.org/10.1111/jmi....

🔬New Methods and Protocols published!

The authors present a methodology for deep learning-based segmentation by transforming high-quality ex situ laboratory data to train models for segmentation of in situ synchrotron data, demonstrated through a metal oxide dissolution study.

"Exploring collagen fibrillogenesis at the nanoscale: Tip-enhanced Raman imaging of protofibrils" by Maria A. Paularie, Emerson A. Fonseca, Vitor Monken, André G. Pereira, Rafael P. Vieira & Ado Jorio is free-to-view here:
doi.org/10.1111/jmi....

🔬 This new open access Journal of Microscopy study presents hyperspectral tip-enhanced Raman spectroscopy (TERS) imaging of collagen protofibrils, offering chemical and structural insights into early fibrillogenesis by acquiring nanoscale molecular profiles of collagen intermediates.

📣Open call for papers: AI in Imaging!

The deadline for papers for the upcoming 'AI in Imaging' special issue has been extended to 31 December 2025.

The special issue will be guest edited by Gianluca Tozzi, Peter Soar & Tuan Nguyen, all from the University of Greenwich.

tinyurl.com/249c6b9e

Methods and Protocols: Journal of Microscopy The <em>Journal of Microscopy</em> is the oldest journal dedicated to the science of microscopy, publishing the latest advances in microscopy techniques and novel areas of application.

- Papers that provide valuable information about optimising methods, protocols and/or data collection.
- Example data should be provided to illustrate the method or protocol.
Please contact Editorial Office Manager Jill Hobbs (journaladmin@rms.org.uk) if you have any questions.
tinyurl.com/39dc3wkd

🔬A collection of our recently published 'Methods and Protocols' papers is available to view online in a virtual issue.

We welcome new 'Methods and Protocols' submissions and ask that these papers are:
- A concise description of a specific technical development, method or protocol.

Thrilled to share that our article in @jofmicroscopy.bsky.social is now accessible online and as open access! If you wonder how to start the analysis of your images, we propose a decision tree to help identify suitable methods. Just follow the link(s)...

#imageanalysis #bioimaging

Sign up for the free virtual event "Cryo-correlative electron tomography for structural biology of complex mixtures" here: tinyurl.com/4ra5swmp

🔬Join Wiley on October 1 for a webinar presented by Dr. Nick Ariotti of the University of Queensland who will present his work creating a robust, structural analysis pipeline of macromolecules and lipid-protein complexes that does not require protein purification.

"From cells to pixels: A decision tree for designing bioimage analysis pipelines" by Elnaz Fazeli, Robert Haase, Michael Doube, Kota Miura & David Legland is free-to-view here: doi.org/10.1111/jmi....

🔬In this new open access paper, the authors explored biologist-programmer interactions to come up with a common language that helps to make connections between how biological phenomena appear in scientific images, and how they are able to translate that into data and analysis strategies.

📣The October 2025 issue of the Journal of Microscopy is now available to view online!

The issue features an invited review "An overview of reliable and representative DVC measurements for musculoskeletal tissues" and 11 original articles: tinyurl.com/3762kh9p

"Automatic identification and quantification of surface nanoscale pore morphology in coals of different ranks based on AFM, SEM and LP-N₂GA" by Dun Wu, Jianghao Wei, Shoule Zhao, Lin Sun & Yunfeng Li is available to view here > doi.org/10.1111/jmi....

🔬This new Journal study employs multiscale techniques including AFM (Atomic force microscopy), SEM (Scanning electron microscopy), and LP-N₂GA (Low-Pressure nitrogen gas adsorption) to systematically analyse the impact of coal rank changes on pore structure and its evolutionary process.

"Quantitative corrections for fluctuation electron microscopy" by J. M. Gibson & M. M. J. Treacy is available to view here > doi.org/10.1111/jmi....

The authors present three experimental corrections for quantitative interpretation that significantly modify conventional approaches. They demonstrate that sampling the nanodiffraction patterns with a pixelated detector removes high-frequency signals and reduces statistical variance.

🔬In this new Journal of Microscopy paper, the authors state that anomalously low values of the normalised variance in fluctuation electron microscopy (FEM) have been frequently reported.

Contrast by electron microscopy in thick biological specimens The contributions of coherent bright-field phase and incoherent dark-field amplitude contrast are investigated for thick biological specimens. A model for a T4 phage is constructed and images simulat...

"Contrast by electron microscopy in thick biological specimens" by Peter Rez, Lothar Houben, Shahar Seifer & Michael Elbaum is free-to-view here: doi.org/10.1111/jmi....

The authors find no advantage for TEM to energy filtering outside the zero-loss peak. A model bacteriophage capsid embedded in a thick amorphous water slab was used to simulate STEM imaging up to 1 micron. Phase contrast STEM methods should be optimal in the intermediate regime.

🔬Specimens in cryo-electron microscopy range from isolated proteins to whole cells. This work addresses the preferred imaging modalities in relation to specimen thickness. Energy loss spectra were recorded in vitreous ice.

Call for Papers - High-Performance Computing and cloud-based solutions for image analysis in life and physical sciences

The DAIM special issue can include invited manuscripts, original research articles, reviews, and methods & protocols.

Deadline for submissions: 28 February 2026

More information can be found here: tinyurl.com/2bn9z6js

The Guest Editors for the issue will be Rocco D'Antuono (The Francis Crick Institute - DAIM RMS, UK), Nicholas Condon (University of Queensland, Australia) and Beth Cimini (Broad Institute, US).