Leicester Institute for Structural and Chemical Biology

Engineering the TCA cycle regulator GarA to increase erythromycin production in Saccharopolyspora erythraea Actinobacteria are important for industrial production of antibiotics, fine chemicals and food and a source of new compounds for drug discovery. Their central metabolism is regulated by a conserved pr...

Congratulations to Helen O'Hare and group on "Engineering the TCA cycle regulator GarA to increase erythromycin production in Saccharopolyspora erythraea" in @microbiologysociety.org
www.microbiologyresearch.org/content/jour...

Anti-Cancer Stem Cell Properties of Square Planar Copper(II) Complexes with Vanillin Schiff Base Ligands in @mdpiopenaccess.bsky.social
www.mdpi.com/1420-3049/30...

Two-dimensional infrared spectroscopy as a tool to reveal the vibrational and molecular structure of [FeFe] hydrogenases [FeFe] hydrogenases are Nature's most efficient catalysts for the cleavage and evolution of molecular hydrogen. Despite decades of research, key aspects of the catalytic cycle and the underlying geome...

Congrats @rmlab.bsky.social and co-authors on "Two-dimensional infrared spectroscopy as a tool to reveal the vibrational and molecular structure of [FeFe] hydrogenases" in Chemical Science pubs.rsc.org/en/content/a...

Leicester Institute for Structural and Chemical Biology | LinkedIn Leicester Institute for Structural and Chemical Biology | 51 followers on LinkedIn. Our researchers in chemistry and biology are collaborating to improve our understanding of the workings of life. | T...

LISCB is now on LinkedIn- join us there and let's make it a useful resource for us all www.linkedin.com/showcase/lei...

Engineered Receptor Capture Combined with Mass Spectrometry Enables High-Throughput Detection and Quantitation of SARS-CoV-2 Spike Protein Mass spectrometry (MS) is a potentially powerful approach for the diagnostic detection of SARS-CoV-2 and other viruses. However, MS detection is compromised when viral antigens are present at low conc...

Engineered Receptor Capture Combined with Mass Spectrometry Enables High-Throughput Detection and Quantitation of SARS-CoV-2 Spike Protein in @pubs.acs.org
pubs.acs.org/doi/full/10....

Structural and functional insights into the interaction between Ku70/80 and Pol X family polymerases in NHEJ - Nature Communications The molecular basis for the enrollment of X family DNA polymerases in non-homologous end joining (NHEJ) is unclear. Here the authors elucidate the structure of Pol λ within the DNA-PK long-range compl...

Structural and functional insights into the interaction between Ku70/80 and Pol X family polymerases in NHEJ @natcomms.nature.com BRCT domain in recruitment and activity of two Pol X members in NHEJ: a unified model for the interaction of Pol X with Ku70/80.
www.nature.com/articles/s41...

congratulations @liam0324.bsky.social, @hopkinsongroup.bsky.social and co-authors for "Proline amide catalyzes formation of toxic crotonaldehyde from acetaldehyde under physiologically relevant conditions" out in Chembiochem chemistry-europe.onlinelibrary.wiley.com/doi/epdf/10....

University of Leicester academic among recipients of prestigious Academy of Medical Sciences Springboard Award | News | University of Leicester

Abhinav KV wins AMS award
le.ac.uk/news/2025/ap...

Terminal hydride formation in [FeFe] hydrogenase: understanding the role of the dithiolate bridgehead [FeFe]-hydrogenases are highly-active hydrogen-conversion biocatalysts using Earth-abundant metals in their active-site. Understanding their mechanism may enable design of catalysts for renewable ener...

Terminal hydride formation in [FeFe] hydrogenase: understanding the role of the dithiolate bridgehead in @chemcomm.rsc.org
Congratulations @rmlab.bsky.social !
pubs.rsc.org/en/content/a...

First BlueSky post! We are hosting a free one day symposium for researchers across academia and industry to share and generate new ideas on how to find molecular glues - 30th April in Leicester. Come along and join the conversation! Register via: www.dovestonlab.co.uk @leicstructbio.bsky.social

How cool is this?? "Structural dynamics of DNA unwinding by a replicative helicase" by #CryoEM Taha Shahid's work at LISCB: le.ac.uk/news/2025/ma...

Identification and inhibition of PIN1-NRF2 protein–protein interactions through computational and biophysical approaches - Scientific Reports Scientific Reports - Identification and inhibition of PIN1-NRF2 protein–protein interactions through computational and biophysical approaches

Identification and inhibition of PIN1-NRF2 protein–protein interactions through computational and biophysical approaches

www.nature.com/articles/s41...

Wellcome awards Leicester scientist £2m to study gene regulation in early development | News | University of Leicester

Hurray for Yolanda Markaki getting a Wellcome Trust Career Development Award! le.ac.uk/news/2025/ma...

celebrating one year of ireacch: 9 april 12-1pm

🎉Celebrating One Year of I-REACCH: 9 April
Join us for project updates, followed by a Q&A session.

Book your place: forms.office.com/e/yYAc2gBrr3

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Attend our workshops on:
-Breaking down barriers
-Creative writing
-Research culture manifestos

Via:
forms.office.com/Pages/Respon...

Interested in the discovery and development of molecular glues with pharmaceutical application? We are hosting a free 1 day forum in Leicester to share ideas and initiate collaborative research projects. Come and join the conversation on Wednesday 30 April 2025!
www.linkedin.com/posts/richar...

Blue-light photodegradation of ferricyanide under protein relevant conditions. Murton P, Timmel C, Mackenzie S, Rodríguez-Maciá P. Dalton Trans. pubs.rsc.org/en/content/a...
A spectroscopic study of blue light photo-induced degradation of ferricyanide under protein-compatible conditions.

Congratulations to @petermoody.bsky.social and Hanna Kwon for pulling this together!

Circular Engineered Sortase for Interrogating Histone H3 in Chromatin Reversible modification of the histone H3 N-terminal tail is critical in regulating the chromatin structure, gene expression, and cell states, while its dysregulation contributes to disease pathogenesis. Understanding the crosstalk between H3 tail modifications in nucleosomes constitutes a central challenge in epigenetics. Here, we describe an engineered sortase transpeptidase, cW11, that displays highly favorable properties for introducing scarless H3 tails onto nucleosomes. This approach significantly accelerates the production of both symmetrically and asymmetrically modified nucleosomes. We demonstrate the utility of asymmetrically modified nucleosomes produced in this way in dissecting the impact of multiple modifications on eraser enzyme processing and molecular recognition by a reader protein. Moreover, we show that cW11 sortase is very effective at cutting and tagging histone H3 tails from endogenous histones, facilitating multiplex “cut-and-paste” middle-down proteomics with tandem mass tags. This cut-and-paste proteomics approach permits the quantitative analysis of histone H3 modification crosstalk after treatment with different histone deacetylase inhibitors. We propose that these chemoenzymatic tail isolation and modification strategies made possible with cW11 sortase will broadly power epigenetic discovery and therapeutic development.

A great collaboration with Phil Cole (Harvard) engineering Sortase to facilitate its transpeptidase activity on histone H3 has been published in JACS. This work developed multiplexed "cut-and-paste" middle-down proteomics with tandem mass tags for quantification.
pubs.acs.org/doi/full/10....

The Anti-Cancer Stem Cell Properties of Copper(II)-Terpyridine Complexes with Attached Salicylaldehyde Moieties in Chembiochem by the Suntharalingam group chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...

MRC AIM studentships with LISCB supervisors on this page now too! Closing date for AIM is 10 January, BBSRC MIBTP is 16 January. Get in touch with any questions on what makes a strong application! hv33@le.ac.uk

Circular Engineered Sortase for Interrogating Histone H3 in Chromatin pubs.acs.org/doi/10.1021/... in @amerchemsociety.bsky.social

Research opportunities | Institute for Structural and Chemical Biology | University of Leicester

A list of all the PhD projects in LISCB advertised with the MIBTP program: le.ac.uk/research/ins.... Get in touch with the listed supervisor(s) or Tennie hv33@le.ac.uk for support in putting in the best possible application.

review by @hopkinsongroup.bsky.social "The biochemistry of the carcinogenic alcohol metabolite acetaldehyde" in DNA Repair www.sciencedirect.com/science/arti...

@leicstructbio.bsky.social

Uncovering the mechanisms of epigenetic regulators using cutting-edge molecular biology and advanced imaging methodologies at University of Leicester on FindAPhD.com PhD Project - Uncovering the mechanisms of epigenetic regulators using cutting-edge molecular biology and advanced imaging methodologies at University of Leicester, listed on FindAPhD.com

🎓 Exciting #PhD opportunity in our lab at @mcbleicester.bsky.social and @leicstructbio.bsky.social. Join the BBSRC-funded MIBTP programme for a 4-year fully funded studentship with comprehensive training in year 1 + research project years 2-4. #PhDSky
#AcademicSky Details: tinyurl.com/4fhavzxh

Interested in difficult to access parts of genetics, cellular & structural biology? Join us in Edinburgh 2-4th April 2025 for "Hidden Cell, Dark Genome"
Regulation in the dark genome
Uncharted proteins, RNA complexity
Atomic structures at cellular scale
Cell diversity
www.ed.ac.uk/c/hidden-cel...