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Right ventricular dysfunctions in type 1 diabetic mice: A longitudinal study

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Substrate stiffness modulates phenotype-dependent fibroblast contractility and migration independent of TGF-β stimulation During wound healing, fibroblasts undergo radical processes that impact their phenotype and behavior. They are activated, recruited to the injury site…

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Substrate stiffness modulates phenotype-dependent fibroblast contractility and migration independent of TGF- β stimulation

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In Vitro Calcification of Bioprosthetic Heart Valves: Method Validation by Comparative Heart Valve Calcification Testing We have developed a unique method for pre-clinical heart valve prostheses evaluation of calcification propensity. Results from the test can directly be translated into improvements of prostheses desi...

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In Vitro Calcification of Bioprosthetic Heart Valves: Method Validation by Comparative Heart Valve Calcification Testing

onlinelibrary.wiley.com/doi/10.1111/...

Vasa vasorum plexus formation in intracranial aneurysm associates with microcalcification and wall thickening Scanning multiphoton imaging of an intracranial aneurysm revealed a vasa vasorum plexus with both positive and deleterious roles.

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Vasa vasorum plexus formation in intracranial aneurysm associates with microcalcification and wall thickening

www.science.org/doi/10.1126/...

Endothelial to mesenchymal transition in cardiovascular diseases: molecular insights and clinical perspectives Abstract. Endothelial to mesenchymal transition (EndMT) is a process whereby endothelial cells transition to adopt a mesenchymal-like fate, e.g. to become

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Endothelial to mesenchymal transition in cardiovascular diseases: molecular insights and clinical perspectives

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Human iPSC-Based in Vitro Cardiovascular Tissue Models for Drug Screening Applications - Current Cardiology Reports Purpose of Review To provide an overview of human induced pluripotent stem cell (hiPSC)-derived cardiovascular lineages and describe their impact on drug testing in vitro. Recent Findings hiPSCs have ...

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Human iPSC-Based in Vitro Cardiovascular Tissue Models for Drug Screening Applications

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The designer cytokine IC7Fc attenuates atherosclerosis development by targeting hyperlipidemia in mice Treatment IC7Fc targets hepatic regulation of lipid metabolism and inflammation, ameliorating atherosclerosis in mice.

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The designer cytokine IC7Fc attenuates atherosclerosis development by targeting hyperlipidemia in mice

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Long-Term Cardiac Electrical Imaging Enabled by Ultrahigh-Resolution Foldable Heart–Machine Interfaces The advancement of electrophysiological mapping for cardiac arrhythmias relies critically on overcoming key interface challenges: long-term biocompatibility, spatial resolution limitations, and mechanical mismatch with dynamic cardiac tissue. To address these, we developed a rapid encapsulation method for liquid metal electrode arrays at the 10-μm scale, enabling the fabrication of ultrahigh-density and flexible sensor arrays (∼28570 sensors/cm2) within 30 min. It constructs a fully foldable, high-density heart–machine interface deployable via minimally invasive access (<3 mm folded diameter). The interface achieves high-resolution imaging (in rat, rabbit, and dog models) of microscale electrical heterogeneity (e.g., 0.2 mm2 reentrant pattern in ventricular fibrillation) while maintaining long-term reliability (100% electrode functionality over 98 days). This technology holds promise for advancing diverse human–machine interfaces for other organs and tissues, enabling the integration of multiple organs and even the whole body for comprehensive health management.

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Long-Term Cardiac Electrical Imaging Enabled by Ultrahigh-Resolution Foldable Heart-Machine Interfaces

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Revisiting tricuspid regurgitation: Historical insights and emerging research perspectives Tricuspid regurgitation (TR), once seen as benign, is now recognized as a serious and increasingly prevalent condition linked to aging and better imaging. Its association with greater morbidity and m....

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Revisiting tricuspid regurgitation: Historical insights and emerging research perspectives

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Multidisciplinary meetings to provide the best cardiovascular care: the European Society for Clinical Investigation In modern medicine, particularly in the field of cardiovascular (CV) diseases, complexity is the rule rather than the exception. Clinical presentations are

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Multidisciplinary meetings to provide the best cardiovascular care: the European Society for Clinical Investigation

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Apoptosis as a strategy for enhancing decellularization and remodeling of tissue-engineered heart valves In situ tissue-engineered heart valves (TEHVs) using human cell-derived tissue engineered matrices (hTEMs) offer a promising solution to current heart…

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Apoptosis as a strategy for enhancing decellularization and remodeling of tissue-engineered heart valves

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Narrative review of endothelial cell metabolism and aberrations in atherosclerosis and peripheral artery disease Several decades of medical research have shown an intricate and definitive connection between dysfunctional endothelium and cardiovascular disorders, including atherosclerosis and peripheral artery di...

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Narrative review of endothelial cell metabolism and aberrations in atherosclerosis and peripheral artery disease

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Pericardial Delivery of Stem Cells: An Emerging Frontier in Myocardial Regeneration for Ischemic Heart Disease Cardiac regenerative therapies have seen limited clinical translation due to persistent challenges in stem cell viability, retention, and demonstration of efficacy. The pericardial space, however, has...

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Pericardial Delivery of Stem Cells: An Emerging Frontier in Myocardial Regeneration for Ischemic Heart Disease

onlinecjc.ca/article/S082...

Chitosan Nanofibrous Dressing Increased Angiogenesis and Anti-inflammatory Response in an Acute Wound Model in Rats: A Comparative Study - Annals of Biomedical Engineering Purpose Chitosan-based materials are promising for wound healing because of their antibacterial efficacy, biocompatibility, and biodegradability, though their healing mechanisms remain unclear. This study explores the cellular and molecular mechanisms of wound healing with chitosan nanofibrous dressings. Methods Thirty-nine Sprague Dawley rats were divided into three groups based on the type of dressings administered: (1) chitosan nanofibrous dressing + transparent film, (2) commercial scaffold + transparent film, and (3) transparent film alone (control). Full-thickness wounds (2 cm × 2 cm) were created on the dorsum, splinted, and covered with dressings. Evaluations at 7, 14, and 21 days included histological analysis, and measurements of TNF-α and iNOS levels in the wounds. Results On day 21, epithelialization was significantly higher in the chitosan group than in the scaffold group (87.5% vs. 42.0%, p = 0.03). TNF-α levels were lower in both treatment groups compared to the controls. In the chitosan group, the CD68+/CD163+ ratio was lower than in the scaffold group (0.28 vs. 0.62, p = 0.037), and blood vessel formation was greater than in the controls. Conclusion These results suggest that chitosan nanofibrous dressings enhance acute wound healing in rats by promoting re-epithelialization, neovasculogenesis, and maintaining low TNF-α levels in the later phases of healing.

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Chitosan Nanofibrous Dressing Increased Angiogenesis and Anti-inflammatory Response in an Acute Wound Model in Rats: A Comparative Study

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Blood pressure, plasma proteins, and cardiovascular diseases: a network Mendelian randomization and observational study AbstractBackground and Aims. The biological pathways leading to elevated blood pressure (BP) and subsequent cardiovascular diseases (CVDs) remain incomplet

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Blood pressure, plasma proteins, and cardiovascular diseases: a network Mendelian randomization and observational study

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Pannexins in the vasculature | American Journal of Physiology-Heart and Circulatory Physiology | American Physiological Society Pannexins (PANX1, PANX2, PANX3) are a family of large-pore, ion and metabolite channels present throughout the blood and lymphatic vascular networks. PANX1 has near-ubiquitous expression in the cardio...

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Pannexins in the vasculature

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Clinical and Experimental Studies of Structural Valve Degeneration of Bovine Jugular Vein Valves: Mitigation with Polyoxazoline Modification - Pediatric Cardiology Bovine jugular vein (BJV) valves are widely used in congenital heart surgery but are limited in durability due to structural valve degeneration (SVD). Here, we examine mechanisms of BJV prosthesis failure and test BJV modification with poly-2-methyl-2-oxazoline (POZ), a polymer shown to inhibit protein and advanced glycation end-product (AGE) absorption. Clinical BJV valve explants (Melody valve, N = 13) underwent Von Kossa staining and immunohistochemistry (IHC) for AGE, carboxymethyllysine (CML), and serum albumin (SA). Collagen structure was analyzed using second-harmonic generation (SHG) imaging. Unmodified and POZ-modified BJV valved conduits underwent 5-week in vitro glycation incubation. Effective orifice area (EOA) and mean pressure gradient (MPG) were recorded weekly using a heart pulse duplicator system. Following incubation, leaflet thickness, IHC, and SHG analyses were performed. In vivo 28-day subcutaneous BJV leaflet implantations were performed in juvenile rats for IHC. Clinical BJV explants demonstrated AGE, CML, and SA infiltration. In vitro glycation induced leaflet thickening (control, 0.03 ± 0.01 vs non-modified, 0.23 ± 0.07 mm, p < 0.001), CML and SA infiltration, and collagen disruption (alignment coefficient: control, 0.76 ± 0.03 vs non-modified, 0.50 ± 0.19, p < 0.01). POZ-modified leaflets remained similar in thickness to unglycated controls. POZ modification reduced CML and SA accumulation and mitigated collagen malalignment. BJV conduits did not reveal clinically significant changes in MPG or EOA. In vivo studies verified POZ modification protected against microcalcification as well as AGE, CML, and SA deposition. BJV valves are subject to SVD-related mechanisms of AGE accumulation, serum protein infiltration, and collagen misalignment. POZ modification mitigates BJV leaflet thickening, AGE and protein infiltration, and collagen disruption.

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Clinical and Experimental Studies of Structural Valve Degeneration of Bovine Jugular Vein Valves: Mitigation with Polyoxazoline Modification

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Preclinical testing of acellular and polyphenol-stabilized arterial xenografts in sheep - Marius M Harpa, Emanuel D Anitei, Dan T Simionescu, Alexandra I Puscas, Raluca I Truta, Ionela Cotfas, Hamida ... Background: Cardiovascular disease is a global leading cause of death, prompting coronary heart diseases and peripheral artery diseases due to atherosclerotic a...

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Preclinical testing of acellular and polyphenol-stabilized arterial xenografts in sheep

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A systems approach to target discovery identifies the role of lncRNA-SPANXA2-OT1 in macrophage chemotaxis

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LRP5, a WNT signalling pathway receptor, and platelet activation AbstractBackground and Aims. Platelets are essential for haemostasis, thrombosis, and inflammation, with their functions controlled by receptor signalling

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LRP5, a WNT signalling pathway receptor, and platelet activation

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A coordinated biomaterial strategy for post-infarction cardiac repair: integrating tailored mechanical reinforcement with hiPSC-derived cardiomyocytes in composite engineered human myocardium for remu... Up to 1 billion cardiomyocytes (CMs) die during myocardial infarction (MI), leading to permanent muscle loss and devasting functional impacts. Biomate…

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A coordinated biomaterial strategy for post-infarction cardiac repair: integrating tailored mechanical reinforcement with hiPSC-derived cardiomyocytes in composite engineered human myocardium for remuscularization

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An integrated 2D framework for quantifying cellular mechanics reveals the impact of juxtacrine Notch signalling on directed collective migration of endothelial cells - BMC Biology Background Collective migration is the coordinated movement of a group of cells—a fundamental process in health and disease. Many models have been developed to study the molecular and physical mechanisms of collective migration. However, the aim of this study is to engineer a flexible in vitro framework that allows for mechanobiological quantification of the separate and combined contributions of individual cell mechanics to the directed migration of a collective. We utilised this framework to understand the role of juxtacrine Notch signalling during collective endothelial migration—an essential process during the formation of new blood vessels (known as angiogenesis). Results This framework enables users to perform high spatiotemporal analysis of migrative behaviour, cell–matrix traction forces, and intercellular forces in different microenvironments. With this framework, we show that Notch inhibited collectives adopt a distinct regime of directed collective migration. Whereas the directionality of migration, traction forces and intercellular forces are not affected by Notch inhibition, we observed spatiotemporal differences in migration speed, traction force magnitude and normal and shear stresses within Notch-inhibited collectives. Conclusions The in vitro framework is a powerful approach for dissecting the mechanisms of collective migration. With this framework, we show that a potential link exists between the juxtacrine signalling of Notch and an increased mechanical cohesiveness among collective cells.

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An integrated 2D framework for quantifying cellular mechanics reveals the impact of juxtacrine Notch signalling on directed collective migration of endothelial cells

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Novel design of 3D printed tumbling microrobots for in vivo targeted drug delivery

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Mechanical cues guide the formation and patterning of 3D spheroids in fibrous environments Abstract. Multicellular spheroids have shown great promise in 3D biology. Many techniques exist to form spheroids, but how cells take mechanical advantage

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Mechanical cues guide the formation and patterning of 3D spheroids in fibrous environments

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Precision medicine requires precision proteomics: Discordance between proteomic and clinical assays in UK Biobank Bhawana Singh, Ioanna Tzoulaki, Manuel Mayr; Precision medicine requires precision proteomics: Discordance between proteomic and clinical assays in UK Biob

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Precision medicine requires precision proteomics: Discordance between proteomic and clinical assays in UK Biobank

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Fabrication and mechanical characterization of near field electrospun bioresorbable vascular grafts with fibrous architecture mimicking the arterial extracellular matrix - Alexandra E Snyder, Jada K S... Cardiovascular disease, arteriosclerosis, is characterized by the thickening of blood vessel (arteries) walls restricting blood flow and is a global health prob...

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Fabrication and mechanical characterization of near field electrospun bioresorbable vascular grafts with fibrous architecture mimicking the arterial extracellular matrix

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Understanding donor heart preservation: Cellular mechanisms and clinical outcomes

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Vasculogenic potential of adipose tissue derived stem cells from patients with chronic spinal cord injury and pressure injuries - Angiogenesis Limited vascularization and ischemia are major contributors to the chronicity of wounds, such as ulcers and traumatic injuries, which impose significant medical, social, and economic burdens. These ch...

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Vasculogenic potential of adipose tissue derived stem cells from patients with chronic spinal cord injury and pressure injuries

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Effect of 3-hydroxyisobutyrate in vivo administration on cardiometabolic disease in ApoE-/- mouse model The valine catabolite 3-hydroxyisobutyrate (3-HIB) is suggested to mediate the uptake of extracellular fatty acids into the cells, thus regulating int…

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Effect of 3-hydroxyisobutyrate in vivo administration on cardiometabolic disease in ApoE-/- mouse model

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Filamin C Cardiomyopathy With Myocarditis in Childhood We present a case of a child presenting with recurrent myocarditis 5 years before developing dilated cardiomyopathy with a novel filamin C truncating variant. Complicated myocarditis in children is co...

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Filamin C Cardiomyopathy With Myocarditis in Childhood

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