Exotoxins lab

I got a plasmid map from another lab as *.dna file 🧪. The SnapGene program can open it. I do not want to install their "free" viewer in my linux system. The solution: OpenCloning opencloning.org . With it, I loaded the .dna file and downloaded the sequence plus a nice map. Thanks OpenCloning team 🤩

Toxin data quality: a critical examination of bacterial exotoxins and animal toxins - BMC Research Notes Objective Existing toxins datasets include a mixture of proteins and toxin peptides. In this study we present two curated datasets of toxic proteins free of associated proteins: bacterial exotoxins an...

Finally 🥳. Our resource paper under Open Science and FAIR data principles 🧪. I never thought that a simple resource paper will take more than 1 1/2 years to get reviewed. But now it is finally out bmcresnotes.biomedcentral.com/articles/10....

🧪Our first paper with Machine Learning under Open Science principles and FAIR data is out! rdcu.be/ezXFx

Mint and tuxedo are super user-friendly. I use both distros for my students, and they never believe that it is linux 🤣

Reproducible analysis of high-throughput data with R Tue, May 6th, 2025

You have analized your data with many steps. Do you want to make the analysis reproducible for others and your future-you? If you are in the LMU Munich, you can come to our workshop on *May 6th*. We will show you how to create workflows in R. Www.pretix.osc.lmu.de/lmu-osim/hig... 🧪

🏆Tuxedo computers 🇩🇪

EU medicines agency quits X, moves to Bluesky The European Medicines Agency (EMA) said on Monday it would no longer post on X and would use rival Bluesky instead, becoming the latest organisation to quit a social media platform that some have criticised for its content.

More of this, please. Welcome to Bluesky, finally crawling out of the Upside Down? No Demogorgons here, the forecast is clear, the science sharper, and the community healthier.

www.reuters.com/world/europe...

Assessing the feasibility and impact of clinical trial trustworthiness checks via an application to Cochrane Reviews: Stage 2 of the INSPECT-SR project Background The aim of the INSPECT-SR project is to develop a tool to identify problematic RCTs in systematic reviews. In Stage 1 of the project, a list of potential trustworthiness checks was created....

« Assessing the feasibility and impact of clinical trial trustworthiness checks via an application to Cochrane Reviews: Stage 2 of the INSPECT-SR project » by a gang of researchers led by @jackwilkinson.bsky.social

FeCl3 solution for NEFF Media For axenic growth of Tetrahymena thermophila in the lab, NEFF media requires a final concentration of 33 µM (see Tetrahymena Stock Center (RRID:SCR_008362)). To guarantee that...

Iron is essential for growth of Tetrahymena in axenic media (prey-free)🧪. Last time we made our stock solution was three years ago. How do we prepare it and store it? The details are now published in @protocolsio.bsky.social under www.protocols.io/view/fecl3-s... and just in time for a new lot 🥼🫡

Yap. You got it. Just make it in a way that his clothes change while going through the pipe. Piping requires some change or process to take place 😉.

Hi Erik, I would like to be added to the microbiologist and/or bioinformatics and/or toxinologist feed. Here is my ORCID: orcid.org/0000-0001-85... and my YouTube channel: youtube.com/@exotoxinsla... thank you for your time. Have a great day!!! 😊

One after another. Like an assembly line. The product of one station will be passed to the next one. Imagine each working station is a function or command of the computer. The term for "at the same time" is parallel processing or parallel programming.

You just need to consider: typing is annoying, so many commands are just abbreviations of the full words: cat for concatenate and pipe for creting a pipeline. I have done the transition recently, so if you want I can help you 😉

Buffer is ready. Hmm. can I sterilize it in the autoclave? When the answer is no, the next best option is to do a sterile filtration. It's not a big deal, but it's easy to do it wrong. Here is our protocol! 🧪 Sterile filtration of liquid solutions  www.protocols.io/view/sterile...

1 M Tris-HCl pH 7.5 for Tetrahymena mating (Stock solution) In this protocol we specify the steps needed to create a 250 ml stock solution of 1M Tris-HCl solution used for the preparation of Tetrahymena thermophila cells for mating. The...

Nothing more annoying than preparing buffers, but extremely necessary if we want to mate ciliates. Here is our protocol for the 1 M Tris-HCl pH 7.5 for Tetrahymena mating (Stock solution) www.protocols.io/view/1-m-tri.... We hope it will be helpful for the community! 🧪 🤲🏻🥼

Oh no please! Then we will never get an update on the manuals. ThermoFischer has not updated a lot of the manuals for MolecularProbes or Gibco kits... let alone the Invitrogen cloning products. Terrible for #OpenScience 😟😞😕

I love to help to solve challenges that come up in the lab, and yesterday I was able to link our protocol for veggie NEFF media in a personal communication (www.protocols.io/view/veggie-...) while discussing differences in flow cytometry results. Life is easier with @protocolsio.bsky.social 🤪🧪

🧪⚛️😂🤣

And it is free! No fee. You just need to cover your own food and lodging.

Mlschool.rostlab.org for inscriptions

We still have places in our Machine Learning School. Anyone in natural sciences or medical background can take part. You just need to be in Munich between the 9th and 12th of December and have the full day available! You will learn the basic of python and will apply it to create your own ML model 🧪

🥺 it is under a paywall 😫

Two more protocols out in @protocolsio.bsky.social. Little by little we can build upon each one to get everything ready for the big ones. This time we have a buffer solution for Quality Control of the AlamarBlue stain, and a classic: Gram staining www.protocols.io/view/gram-st....

Question to the community 🧪: in the last 4 years, I have tutored PhD students from other labs and institutions. Too many have had a lot of problems because of bad PIs, and I do mean really bad scientist and people. Some are under investigation. How can I warn future students about them?

Online marketplace for animal samples could cut waste and save lives First-of-its-kind platform will allow scientists to share tissues, organs, and other biological materials

First-of-its-kind Online marketplace for sharing tissues, organs and other biological materials. @science.org

By @arukon0.bsky.social #scisky #news #science

www.science.org/content/arti...

These are outstanding resources for anyone engaged in creating scientific knowledge. 🧪🛟🩺

Eric Partaker on LinkedIn: It's a HUGE mistake to promote a toxic manager. → And it starts by… | 475 comments It's a HUGE mistake to promote a toxic manager. → And it starts by ignoring poor behavior. As Justin Wright recently share with this post, a toxic manager is… | 475 comments on LinkedIn

www.linkedin.com/posts/eric-p... Really interesting view... how would you measure trustworthiness? 🧪

New guide on trusted repositories: Promoting open science and meeting grant requirements A new study provides a list of trusted repositories across different fields of research, with an assessment of their readiness to facilitate compliance with the Horizon Europe requirements.

At erc.europa.eu/news-events/..., they link a document with the list of criteria on how to evaluate if a data repository complies with the new Horizon Europe grants. A list of trusted repositories should be in the document, too 🧪. Make you data FAIR!

www.linkedin.com/posts/ivan-k...

Yes!!! Today, we finally managed to do the update to the most recent version. Thank you to the team for the great documentation. I am looking forward to discover the new features. We have all our chemicals, plasmids, and protocols there!