Aidan Reynolds (Chemistry Lego Guy)

This is so helpful, thank you!

C. elegans! We decided to transfer them to a colleague’s -80 and hope they revive. Another C. elegans researcher said as long as they don’t thaw they should be okay but we’re still really concerned.

This is our first time with worms in this situation so we’re really in the dark. I appreciate any advice for how to handle worm frozen stocks when temperatures shift this much!

WORM EMERGENCY!!! Our -80 compressor is failing and we’re not sure what will happen to our worm stocks at the current temp (-45C). They’ve been at this temp for 16 hours and we’re contemplating reviving all strains not currently in use to save them, or move them to a new -80. Anyone have advice??

That’s me! I’ll be highlighting some cool work with @brukercorporation.bsky.social’s Trapped Ion Mobility Spectrometry (TIMS) showing some neat isomer work during the workshop, and iprm-PASEF data at a poster (WP 266) so come see what myself and my lab are up to!

Finally getting good fluorosmurf assay results since I cut my fluorescein percentage from 2.5% -> 1%. 🪱

Weird question and I fully expect to not get a good answer, but when does the conference date for MAPSS usually get announced? My family wants to visit in late June/July and they go to campgrounds during their stays and they want to book early. I don’t want to tell them a date MAPSS might be on. 😅

Any C. elegans fan?

Woohoo! That’s my PI (and that will be some of my data presented)! 😆

Now i need to hurry up and publish my imaging results soon, too 🤣

Visualization and spatial mapping of PFAS in the edible storage root of radish Plants, as key components of trophic networks, play a critical role in the bioaccumulation of PFAS and their transfer to higher trophic levels. This s…

Super excited to see someone else try my method for analyzing PFAS using MALDI-TOF with TIMS! I’ve been having great results behind the scenes imaging sulfonates in mouse tissues and it’s awesome to see plant imaging done as well. www.sciencedirect.com/science/arti...

Nothing could have prepared me for how tiny microchannel plates are! Got to replace them in an icpTOF S2 and it was awesome. #teammassspec

#Celegans #Worms - I'm guessing sending whole labs to big conferences might be too expensive for many labs this year. I want to highlight this meeting will be FREE. VAI will cover all costs and lunch and if people still want night networking I will set something up if enough are interested.

Collaborators didn’t like the manuscript. Redoing a million experiments now. Delayed until March. Feels like the last few months of rushing this paper for grants and fellowship deadlines out were for nothing.

Instant repost from me! I’ll log in and support it through the webpage when I get off work, too!

This is from my lab! I’m hoping my preprint will be out later this week or Monday! Seems like with grant submissions out of the way we’re finally submitting these papers. 🤣

Final product

Taking a break from designing lego mass spectrometers and made my stepdad (an electrical engineer who worked in renewable energy) a solar farm. Needed a mental break from submitting a P41 and editing this manuscript. Thank god both of those are done next week.

Unfortunately I can’t go to #Worm2025. I’ll instead be sticking to my midwest roots and going to MAPSS and a midwest C. elegans conference at Van Andel institutute in Grand Rapids, MI.

Updating 2 more supplemental figures tomorrow morning and finishing the abstract and conclusion before revising for the next week… so close to being done with this paper 🙏

Pairing microscopy images with my mass spec images… whoof this is tedious when I have 80 images of worms. Going to be a long Sunday for me 🤣

Thank you, I’ll read that article today!

This helped a lot, thank you! I usually sync at the end of the work day and transfer to plate the following morning (~18 hours) in M9 for convenience reasons but I almost deviated from that this morning to do a 24 hour M9 incubation post-sync. Glad I avoided that mistake! 😆

I did a bleach synchronization procedure. Not my favorite sync approach but I needed a lot of worms for some of my recent experiments. 😬

Thank you!! I feel better about my sampling then. 😊

Also sorry if this is a weirdly basic question, I only noticed this variation since I do imaging and size sort of matters here.

Is this just population variance where some of these smaller worms are actually young adults and not yet gravid? I’m trying to wait 4 hours after these worms should be all considered “Day 1 gravid.” Or is this population variance in that there is heterogeneity in the final sizes of grown worms. 2/2

I need some #Celegans help part people. I have synchronized populations reaching day 1 gravid adulthood and I see differences in length by up to ~100um. Does C. elegans grow a little more once becoming gravid to reach its ~1mm length or… 1/2

Folks I may have just found the Lego parts to make a moveable C. elegans. Stay tuned for a potential build in the coming few days.