@manu-lera.bsky.social
Working on OpenCloning, an Open Source web application to plan and document cloning. https://opencloning.org/ Open Source in science enthusiast. Previously PomBase, yeast genetics, image analysis and cytoskeleton.
🚀 OpenCloning now has a documentation page!
Whether you're getting started or diving deep, find everything you need to make the most of OpenCloning, including a powerful search function to quickly find exactly what you’re looking for.
#OpenCloning #OpenSource #SynBio
🔗 docs.opencloning.org
😍🚀🚀🚀🚀
31.07.2025 11:58 — 👍 0 🔁 0 💬 0 📌 0
PomBase introduces two new interactive views for GO-CAM pathway models: Summary Maps (high-level, visual overview) and Mega-Models (connected models). Check them out here: lnkd.in/gfxNRdua
31.07.2025 09:07 — 👍 5 🔁 3 💬 1 📌 0The kit is available from @addgene.bsky.social
www.addgene.org/kits/gasser-...
🔬 I added a template for the #GoldenPiCS #MoClo kit to #OpenCloning!
⚡ Speed up your design when cloning in #Kphaffii!
🧪 Simulate plasmid assembly
🧬 …and integration into the genome!
👉 Check it out here: opencloning.org 🌍
🎥 youtu.be/BadWzLjojb4
🧪 Maybe it's time to give #OpenCloning a try!
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
👨🔬 Built by a fellow researcher — for researchers!
👉 Check it out: bsky.app/profile/manu...
A paper I have been working on with Oliver Lemke in the Ralser lab has just come out: www.nature.com/articles/s41...
We used predicted structures to gain insight into the evolution of metabolic proteins in yeast.
A bit more in this linked-in post (www.linkedin.com/posts/benjam...).
👏👏👏
03.07.2025 08:45 — 👍 1 🔁 0 💬 0 📌 0
Superhappy to share the last paper from my PhD in @litinice.bsky.social lab. We evolved yeasts with humanized telomeric repeats and discovered that they adapt through amplification of telomere-binding proteins and inactivation of the DNA damage response.
www.biorxiv.org/content/10.1...
FlyBase needs your help!
Because of recent changes to government funding, the NIH grant that supported FlyBase has been "terminated." flybase.org
PhD position available in Markus Ralser (Charite, Berlin), for a project about using spatially resolved metaproteomics to study microbial metabolic interactions 👇
30.05.2025 08:46 — 👍 4 🔁 4 💬 1 📌 0
The war on science in the US is already having an effect on private sector research like AlphaFold. Bears repeating but the private sector builds on top of things created by academic research for the public good. This hurts everyone.
28.05.2025 10:18 — 👍 240 🔁 106 💬 2 📌 12Reposting as I hear about a German Pubmed-like initiative: let's build on Europe PMC which is already great
29.05.2025 12:33 — 👍 41 🔁 12 💬 2 📌 0Hi @retropz.bsky.social thanks! Happy to hear if you have any feedback, or if you would need some support for your users to get started.
19.05.2025 12:47 — 👍 2 🔁 0 💬 0 📌 0
Join us next week, 15th May, at UCL!
🧬 Using synthetic biology tools to optimise Cas9 expression in mosquito gene drives
Dr. Moeez Khan (Windbichler lab, ICL)
⚙️ Writing the future with synthetic DNA
Laurence Humphrey (Twist Biosciences)
events.humanitix.com/london-synbi...
Join us in London (12-13 November) for the 10th anniversary of SynBio UK, the premier conference for synthetic and engineering biology.
Organised this year by @biochemsoc.bsky.social and colleagues at @ucl.ac.uk.
www.eventsforce.net/biochemsoc/s...
#synbio
I got hit by some rather sudden and extreme financial hardship so if anyone is in need of remote wetlab contract research, strictly BSL1, do let me know. Currently scrambling for gigs.
Plant, Bacterial, Archaeal Non-model Bioeng
Custom Lab Hardware
Turn Key Genetic Design
Please repost for reach 💚
I built a database with mined gateway plasmids from addgene. Maybe it can be helpful.
bsky.app/profile/manu...
🚀 Just added support for Cre/Lox recombination to hashtag#OpenCloning! 🧬✨
👇 Check out an example showing excision of a plasmid from a locus and re-integration using classic LoxP sites.
👉 Try it here: opencloning.org
🎞️ Demo video: www.youtube.com/watch?v=n0he...
🧬 If you are in London and you want to learn about a free & Open Source SnapGene/Benchling alternative that also supports automation 🤖, come to our next London Synbio Network meeting this Thursday 24th in Imperial!🎓
🎤 I’ll be giving a talk about #OpenCloning!
events.humanitix.com/copy-of-lond...
Come join us for the next London Synbio Network meeting on Thursday the 24th of April in Imperial.
I am giving a talk about #OpenCloning, so join if you want to hear more about Open Source and automation of DNA engineering.
👏👏👏
09.04.2025 07:28 — 👍 1 🔁 0 💬 1 📌 0I spent a very nice couple of days at the "Hidden Cell, Dark Genome" conference in Edinburgh last week ... and even got to win a supercute eukaryotic cell 🙂 Thanks to the organisers for arranging the meeting!
09.04.2025 06:39 — 👍 15 🔁 4 💬 2 📌 1FlyBase.org is the featured #GlobalBiodata Global Core Biodata Resource of the week: x.com/i/status/190...
The Global Biodata entry for FlyBase is here:
www.linkedin.com/pulse/flybas...
#openscience
Just got your sequencing results from Full Circle Labs? 🧬✅ Use #OpenCloning to easily align your Nanopore (or Sanger) data with your constructs and verify your sequences in seconds!
🔬 Free & Open Source
🛠️ Supports most cloning methods
🔗 opencloning.org
#SyntheticBiology #MolecularBiology
#OpenCloning is an amazing tool, and we've integrated it into the next version of eLabFTW!
01.04.2025 14:45 — 👍 3 🔁 2 💬 0 📌 1@plasmidsaurus.bsky.social @fclabs.bsky.social 👋
01.04.2025 13:58 — 👍 0 🔁 0 💬 0 📌 0You can now ✅ verify your #OpenCloning sequences using sequencing data 🧬 (Sanger or Nanopore). Just load your files 📂 and see where they align! 🎯
#OpenCloning is free & Open Source, and supports most cloning methods! 🔬🛠️
👉 Try it here: opencloning.org
🎞️ Demo video: youtu.be/n0hedzvpW88?...
eLabFTW is featured in the News Digest RDM this week :)
31.03.2025 09:49 — 👍 2 🔁 2 💬 0 📌 0
