Manuel Lera-Ramirez

✨ Last week at #ESSL2025 I presented a poster on #OpenCloning 🧬

🔥 Crowd favorite feature: pull gene sequences directly from NCBI — no copy-paste needed!

🎥 Here’s a quick demo

🔗 Visit opencloning.org

London SynBio Network 10 London SynBio afterwork talks and networking event on Thursday 25th of September

🎉 London SynBio Network is back! Join us on 25th of September.

🧬 Surpassing Nature – Metabolic engineering for novel biological CO2 fixation routes
Ari Satanowski

💐 Open Flower: designer flowers for art and education
Nick Desnoyer

➡️ Register at: events.humanitix.com/london-synbi...

🧬 Are you a wet lab scientist doing cloning or DNA engineering, or a bioinformatician curious about molecular biology? 🌱 Passionate about Open Source and Open Science?

Join the pydna hackathon: dtu.events/pydnahackath...

🌱 Whether you're new to Python or an expert, there's a place for you!

🚀 OpenCloning now has a documentation page!

Whether you're getting started or diving deep, find everything you need to make the most of OpenCloning, including a powerful search function to quickly find exactly what you’re looking for.

#OpenCloning #OpenSource #SynBio

🔗 docs.opencloning.org

😍🚀🚀🚀🚀

PomBase introduces two new interactive views for GO-CAM pathway models: Summary Maps (high-level, visual overview) and Mega-Models (connected models). Check them out here: lnkd.in/gfxNRdua

The kit is available from @addgene.bsky.social

www.addgene.org/kits/gasser-...

YouTube video by Genestorian GoldenPiCS with OpenCloning: Step-by-Step MoClo Cloning Tutorial

🔬 I added a template for the #GoldenPiCS #MoClo kit to #OpenCloning!
⚡ Speed up your design when cloning in #Kphaffii!

🧪 Simulate plasmid assembly
🧬 …and integration into the genome!

👉 Check it out here: opencloning.org 🌍
🎥 youtu.be/BadWzLjojb4

🧪 Maybe it's time to give #OpenCloning a try!

✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
👨‍🔬 Built by a fellow researcher — for researchers!

👉 Check it out: bsky.app/profile/manu...

The role of metabolism in shaping enzyme structures over 400 million years - Nature By combining structural biology and evolutionary genomics analyses, the evolution of enzymes over 400 million years is shown to be governed by catalytic function, metabolic network architecture, cost ...

A paper I have been working on with Oliver Lemke in the Ralser lab has just come out: www.nature.com/articles/s41...

We used predicted structures to gain insight into the evolution of metabolic proteins in yeast.

A bit more in this linked-in post (www.linkedin.com/posts/benjam...).

👏👏👏

The adaptive molecular landscape of reprogrammed telomeric sequences Telomeric sequences vary across the tree of life and intimately co-evolve with telomere-binding protein complexes. However, the molecular mechanisms allowing organisms to adapt to new telomeric sequen...

Superhappy to share the last paper from my PhD in @litinice.bsky.social lab. We evolved yeasts with humanized telomeric repeats and discovered that they adapt through amplification of telomere-binding proteins and inactivation of the DNA damage response.

www.biorxiv.org/content/10.1...

FlyBase needs your help!

Because of recent changes to government funding, the NIH grant that supported FlyBase has been "terminated." flybase.org

PhD position available in Markus Ralser (Charite, Berlin), for a project about using spatially resolved metaproteomics to study microbial metabolic interactions 👇

The war on science in the US is already having an effect on private sector research like AlphaFold. Bears repeating but the private sector builds on top of things created by academic research for the public good. This hurts everyone.

Reposting as I hear about a German Pubmed-like initiative: let's build on Europe PMC which is already great

Hi @retropz.bsky.social thanks! Happy to hear if you have any feedback, or if you would need some support for your users to get started.

London SynBio Network 9 London SynBio afterwork talks and networking event on Thursday 15th of May

Join us next week, 15th May, at UCL!

🧬 Using synthetic biology tools to optimise Cas9 expression in mosquito gene drives
Dr. Moeez Khan (Windbichler lab, ICL)

⚙️ Writing the future with synthetic DNA
Laurence Humphrey (Twist Biosciences)

events.humanitix.com/london-synbi...

Join us in London (12-13 November) for the 10th anniversary of SynBio UK, the premier conference for synthetic and engineering biology.

Organised this year by @biochemsoc.bsky.social and colleagues at @ucl.ac.uk.

www.eventsforce.net/biochemsoc/s...

#synbio

I got hit by some rather sudden and extreme financial hardship so if anyone is in need of remote wetlab contract research, strictly BSL1, do let me know. Currently scrambling for gigs.

Plant, Bacterial, Archaeal Non-model Bioeng
Custom Lab Hardware
Turn Key Genetic Design

Please repost for reach 💚

I built a database with mined gateway plasmids from addgene. Maybe it can be helpful.

bsky.app/profile/manu...

🚀 Just added support for Cre/Lox recombination to hashtag#OpenCloning! 🧬✨
👇 Check out an example showing excision of a plasmid from a locus and re-integration using classic LoxP sites.

👉 Try it here: opencloning.org
🎞️ Demo video: www.youtube.com/watch?v=n0he...

London SynBio Network 8 London SynBio afterwork talks and networking event on Thursday 24th of April

🧬 If you are in London and you want to learn about a free & Open Source SnapGene/Benchling alternative that also supports automation 🤖, come to our next London Synbio Network meeting this Thursday 24th in Imperial!🎓

🎤 I’ll be giving a talk about #OpenCloning!

events.humanitix.com/copy-of-lond...

Come join us for the next London Synbio Network meeting on Thursday the 24th of April in Imperial.

I am giving a talk about #OpenCloning, so join if you want to hear more about Open Source and automation of DNA engineering.

👏👏👏

I spent a very nice couple of days at the "Hidden Cell, Dark Genome" conference in Edinburgh last week ... and even got to win a supercute eukaryotic cell 🙂 Thanks to the organisers for arranging the meeting!

FlyBase.org is the featured #GlobalBiodata Global Core Biodata Resource of the week: x.com/i/status/190...

The Global Biodata entry for FlyBase is here:
www.linkedin.com/pulse/flybas...

#openscience

Just got your sequencing results from Full Circle Labs? 🧬✅ Use #OpenCloning to easily align your Nanopore (or Sanger) data with your constructs and verify your sequences in seconds!

🔬 Free & Open Source
🛠️ Supports most cloning methods

🔗 opencloning.org

#SyntheticBiology #MolecularBiology