Meru Sadhu

Just imagining research talks in antiquity.

EUCLID: …thus, there does not exist a rational number whose square is equal to 2.

HIPPOCRATES: [grumbling] I fail to see the clinical relevance of any of this

26.09.2025 13:01 — 👍 3    🔁 0    💬 0    📌 0

Final version of our paper out now on how high-throughput methods can be useful for answering everyday “low-throughput” genetic questions!

11.09.2025 03:08 — 👍 6    🔁 1    💬 0    📌 0

The 90s was more my radio era than albums, but I’d say each of these are perfect in their own way: RATM, Midnite Vultures, Rangeela, Blackout! (Would have also said 2001, but the skits are terrible.)

06.09.2025 04:05 — 👍 1    🔁 0    💬 0    📌 0

Now out officially, with the entire kit on @addgene.bsky.social. Great collaboration with @merusadhu.bsky.social, with more to come.

20.08.2025 12:51 — 👍 2    🔁 1    💬 0    📌 1

What did you think of Gyokeres? Didn’t seem very threatening. Could just need more time to bed in.

18.08.2025 03:01 — 👍 0    🔁 0    💬 0    📌 0

Microscopy images showing that correct localisation of either Ktd1 or Snc1 requires the COG complex.

Kamilla Laidlaw, Hatwan Nadir, Chris MacDonald @yorkyeast.bsky.social and team @biologyatyork.bsky.social discover that killer toxin K28 resistance in yeast relies on COG complex-mediated trafficking of Ktd1.
journals.biologists.com/jcs/article/...
Article: journals.biologists.com/jcs/article/...

21.07.2025 12:25 — 👍 11    🔁 5    💬 1    📌 1

I’m really rooting for this approach to catch on! Of the papers I’ve been involved in, this is probably the one I most feel is contributing a different way of thinking. Big shoutout to the co-first authors, Molly Monge (now an MD/PhD student at Cornell) and Simone Giovanetti.

14.07.2025 15:18 — 👍 3    🔁 0    💬 0    📌 0

“High throughput” doesn’t have to mean hard/expensive! At its heart, the difference is that instead of picking a few clones of your transformation, you take all the colonies. And with low sequencing costs and the possibility of pooling sequencing, it will generally be affordable.

14.07.2025 15:18 — 👍 1    🔁 0    💬 1    📌 0

Second, background mutations. Often in low-throughput approaches, each strain is generated once and split into replicate cultures for experiments. It makes the experiment a lot easier, but any background mutations are shared between replicates! Again, bulk methods can help!

14.07.2025 15:18 — 👍 1    🔁 0    💬 1    📌 0

First, replicates. It’s hard to do low-throughput experiments on even a modest number of samples with more than a small number of replicates per sample. Not a problem if you make your replicates in bulk using barcodes and test your hypothesis in high throughput!

14.07.2025 15:18 — 👍 1    🔁 0    💬 1    📌 0

Validate User

New paper from my lab! We describe our idea that high-throughput pooled experimental methods – typically used to test thousands of hypotheses at once – also have huge potential to help in “everyday” experiments testing one or a few focused hypotheses. Why? Two reasons: doi.org/10.1093/g3jo...

14.07.2025 15:18 — 👍 16    🔁 5    💬 1    📌 0