Pablo Munoz

I had the opportunity to test the Leica Stellaris Falcon system. Beautiful. FLIM is my favorite technique 😍

Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social

Thanks you, Jake. Yeah, I saw the pattern but doesn't stain all the axon of the mossy fibers. Just that area. Do you think are axonal tips doing synapse?

#FluorescenteFriday any expert on hippocampus that can gave an opinion about why a protein is accumulated in the DG and Ca3 ? ROi show region of interest of a protein enrichment which doesn't codistributed with soma

Thanks, I will check :)

Thanks 🫶, this is Lamp1 staining

Early postnatal mouse hippocampus (P0)
Nuclei (yellow), βIII-Tubulin (red), and lysosomes (cyan) reveal the intricate architecture of developing neurons.
Excited to observed how lysosomal dynamics shape early brain development! 🧠✨ #Neurodevelopment #Microscopy #Hippocampus #ZEISS #confocal #mouse

Happy to share the latest e-print from the lab!

papers.ssrn.com/sol3/papers....

Primary neuronal cultures are the most beautiful 😍

Brain imaging #FluorescentMonday

A New Year's rant, for 2025 😊:

a man wearing a hat and a necklace is saying thank you ALT: a man wearing a hat and a necklace is saying thank you

🙌

A Christmas neurons for these holidays #FluorescenceFriday

a man in a black jacket is standing in the snow and says `` my watch has ended '' . ALT: a man in a black jacket is standing in the snow and says `` my watch has ended '' .

I applied to 3 grants this year, got none. Not being an US citizen I could only applied to 3. Even having a good CV and good project. seems not enough. I think a fail N3 is the way to cademia to give me a good bye.

Haha we have to ask Jackson laboratories in Hogwarts

I sent this image to an art in science contest at Yale. They never choose my images so I share it here 😅 Behold the Phoenix brain #FluorescenceFriday

Bumping this one to the 🧪 feed to get some well-deserved eyeballs on it. Give this very cool thread a read and follow this unique scientist @erturklab.bsky.social who continues to put out outstanding work across multiple domains of inquiry. #lightsheet

Sex-specific perturbations of neuronal development caused by mutations in the autism risk gene DDX3X https://www.biorxiv.org/content/10.1101/2024.11.22.624865v1 DDX3X is an X-linked RNA helicases that escapes X chromosome inactivation and is expressed at higher

Sex-specific perturbations of neuronal development caused by mutations in the autism risk gene DDX3X https://www.biorxiv.org/content/10.1101/2024.11.22.624865v1

This work is a big step forward in understanding DDX3 and its role in neurodevelopment.

This is a fantastic paper. It's great to see sex differences clearly quantified instead of combining data from both sexes. The detailed look at morphology using a face-validity model, unlike previous studies.

#FluorescenceFriday Growt cone of a cortical neuron at stage 3.

🧪🔬💻 Will be posting our best-of videos from the past 10 years at the intersection of microscopy, computer science, and biology. My first @naturemethods paper: "ClearVolume: open-source live 3D visualization for light-sheet microscopy" (2015)
www.nature.com/articles/nme...

Nissl staining mouse brain hippocampus 🤩

When I was working in Washington DC. I volunteer for an MRI scan of my brain. I have the CD but it is cool to see my own brain

Yeah, they renovated and just opened some months ago. :)

The Peabody museum at Yale had one of my favorite animals from the miocene. The bird terrors (Phorusrhacidae). The ate horses in Southamerica.