Andrea Dallapè

I knew Texas was big, but I didn’t know it encompasses 4 states

Lovely.
So many US states I never heard of before, I definitely have to study an US map much better

High resolution CryoEM maps of RNA assemblies

Not my area at all, but how cool are these cryoEM structures of purely RNA-based assemblies!
www.nature.com/articles/s41...

Cryo-EM structures of Egl-BicD-RNA complexes reveal how diverse mRNAs are selected for subcellular localization Localization of mRNAs is a widespread mechanism for controlling where proteins operate in cells and underpins many fundamental processes, from embryonic patterning to synaptic plasticity. This spatial...

Here is the correct link: www.biorxiv.org/content/10.1...

Cryo-EM structures of Egl-BicD-RNA complexes reveal how diverse mRNAs are selected for subcellular localization www.biorxiv.org/content/10.1101/2025.08.02.668268v1 #cryoEM

Check out our new preprint on the discovery of a molecular switch in NAC that mediates nascent chain sorting on the ribosome and prevents mitochondrial protein mistargeting by SRP. A great collaboration with the Shan Lab @Caltech and the Qi Lab @UVA: www.biorxiv.org/content/10.1...

With boost to NIH budget, Senate panel rejects Trump’s plan to slash agency Republicans on spending committee push back against proposed reorganization and overhead cuts

The National Institutes of Health’s budget, and structure, would be preserved under a Senate bill for the 2026 fiscal year.

Sub-3 Å resolution protein structure determination by single-particle cryo-EM at 100 keV Cryoelectron microscopy (cryo-EM) has transformed structural biology by providing high-resolution insights into biological macromolecules. We report s…

Now out in Structure: Paper on high-resolution #cryoEM using the 100 kV Tundra cryo-TEM by Dimple Karia, Adrian Koh, @abhaykot.bsky.social, @lingboyu.bsky.social, @landerlab.bsky.social and many others. Happy to have contributed to this effort. Check it out:
www.sciencedirect.com/science/arti...

FlyTomo: A streamlined software for on-the-fly cryo-ET data processing and diagnosis https://www.biorxiv.org/content/10.1101/2025.07.24.666517v1

New preprint: In situ Architecture of #Plasmodesmata.

Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores.

🔗 doi.org/10.1101/2025...

🧪🧵1/n
#teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM

Structural and cell biology NPG publication two-fer today: lysosome signaling structural biology tinyurl.com/mt3uc96k with @robzonculab.bsky.social and mitophagy initiation tinyurl.com/3rpjyfjt with @martenslab.bsky.social @lazaroulab.bsky.social and @hummerlab.bsky.social

Not bored, amazed and curious

Twist and Scout: Analysis and Curation of Particles in Cryo-Electron Tomography Using TANGO www.biorxiv.org/content/10.1101/2025.07.17.665296v1 #cryoEM

Choreography of human mitochondrial leaderless mRNA translation initiation pubmed.ncbi.nlm.nih.gov/40672251/ #cryoEM

That is his favourite foot right? Seems normal to me he is having a conversation with it

Structure of an archaeal ribosome reveals a divergent active site and hibernation factor - Nature Microbiology Sequence and structural analyses reveal a divergent peptidyl transferase centre and a hibernation factor in archaea.

Structure of an archaeal ribosome reveals a divergent active site and hibernation factor | Nature Microbiology https://www.nature.com/articles/s41564-025-02065-w

Wow!

Your sleep pressure is powered by your mitochondria! Sleep, energy, and ageing are all connected through mitochondrial bioenergetics in neurons. Brilliant work from Gero Miesenböck’s lab, just out in Nature @nature.com
www.nature.com/articles/s41...

New paper out in Mol Cell, where we show how N-myristolytransferases lipidate proteins on the translating ribosome!
Continuing our successful collaboration with DeuerlingLab @uni-konstanz.de & Shu-ou Chan @caltech.edu
@snsf.ch @ethz.ch
www.cell.com/molecular-ce...

A yellow microtubule

85 miles/week, $.42 gain… ~$36 week…
~$1700 year. I’m doing my part 💪🤣

In situ protein crystallography: a single-crystal electron diffraction pipeline for structure determination inside living cells. www.biorxiv.org/content/10.1101/2025.07.08.663504v1 #cryoEM

Cryo-electron tomography: Challenges and computational strategies for particle picking pubmed.ncbi.nlm.nih.gov/40639056/ #cryoEM

Key numbers in cell biology

Having a sense of scale helps to think more rigorously and realistically about biological systems.

A practical look at cryo-electron tomography image processing: Key considerations for new biological discoveries Cryo-electron tomography (cryo-ET) enables 3D visualization of complex biological environments without the need for purification, thereby preserving t…

Happy to say that my Current Opinion in Structural Biology article came out today. I hope it helps to guide to you in using cryo-ET to make new biological discoveries!

www.sciencedirect.com/science/arti...

CryoET tomogram with the gap junction (green), ribosomes (cyan) and microtubules (pink)

In our latest preprint on bioRxiv, we present the in situ structure of
the human gap junction at 14 Å resolution using cryo-ET.

The structure provides a blueprint for investigating how connexin
regulation shapes intercellular communication in health and disease.

Woah! Sick reconstitution work showing that MAP6d1 recruits tubulin to form B tubules on A tubule templates, and ALSO intra-luminal protofilaments, which apparently are present in neuronal microtubules.
So not only actin inside microtubules, but also additional tubulin protofilaments?!? Wild!

Structural insights into transcriptional regulation by the helicase RECQL5 - Nature Structural & Molecular Biology Florez Ariza and Lue et al. use cryo-electron microscopy to investigate how the RECQL5 helicase regulates transcription. Their structural findings suggest that RECQL5 can modulate RNA polymerase II’s ...

Our work, co-led by @alfredo0712.bsky.social and @nlue8.bsky.social is out in @natsmb.nature.com Shout out to coauthors P.Grob, B. Kaeser, J.Fang, and S. Kassube for their contributions and thanks to the reviewers for the suggestions that led to the final work.
www.nature.com/articles/s41...

High-resolution cryo-EM structures of small protein-ligand complexes near the theoretical size limit https://www.biorxiv.org/content/10.1101/2025.06.30.662489v1

This shows it better:

phenix-online.org/phenixwebsit...

Phenix.douse is pretty good for automated water building, although I would make the cutoffs a bit more stringent to reduce false positives (increasing sphericity and CC cutoffs to ~0.8 seems to do the trick)