Charles Bayly-Jones

Thank you Wai-Hong, I'm stoked. Very exciting times, but quite stressful also! 😂

We’re looking for motivated students eager to help pioneer new techniques in the lab.

Students interested in learning #CryoEM and protein biology, and who bring complementary backgrounds (e.g., mass spectrometry, #CRISPR /lentiviral, or fluorescence microscopy), are especially encouraged to apply.

I’ve officially started my lab at Monash University! 🎉

We’re diving into #cellgrowth and #lysosome biology, through the lens of molecular #structure and mechanism.

I’m thrilled to say that PhD student scholarships are available—so please share widely and get in touch if you’re interested. 🤩

Tried it. Its awesome. Shame the code isn't available. Webserver only, how long will it last??

I've heard this position articulated by a lot of people, but I think this is hard to make precise. Much of this tracks a problem we already know in science: standards of authorship are not stable and there are many difficult edge cases.

Consider these cases...

Physically Grounded Generative Modeling of All-Atom Biomolecular Dynamics www.biorxiv.org/content/10.64898/2026.02.15.705956v1 #cryoEM

Metadynamics meets diffusion-based structure prediction: the same trick that drives enhance sampling of MD trajectories can be adapted and applied as steering terms during generation to sample diverse conformations. Seems obvious in hindsight!

A small polymerase ribozyme that can synthesize itself and its complementary strand The emergence of a chemical system capable of self-replication and evolution is a critical event in the origin of life. RNA polymerase ribozymes can replicate RNA, but their large size and structural ...

How could a simple self-replicating system emerge at the origins of life? RNA polymerase ribozymes can replicate RNA, but existing ones are so large that their self-replication seems impossible. Could they be smaller?

Excited to share our latest work in @science.org on a new small polymerase.
1/n

Structure and function of the yeast amino acid-sensing SEAC–EGOC supercomplex Nature Structural & Molecular Biology, Published online: 12 February 2026; doi:10.1038/s41594-026-01746-2Tafur et al. determined the cryo-electron microscopy structure of the SEA complex (GATOR) bound to its substrate, the EGO complex (Ragulator–Rag), and showed that its GAP activity is essential for both rapid inactivation and reactivation of TORC1.

New online: Structure and function of the yeast amino acid-sensing SEAC–EGOC supercomplex

New preprint from the Robertson Lab! We used AF ensemble generation for rigidity filtering with generative design to make cryoEM fiducials, enabling rapid inactive states of four drug targets and a beta2 extracellular fiducial to study GPCR activation!
www.biorxiv.org/content/10.6...

Construction of complex and diverse DNA sequences using DNA three-way junctions - Nature Sidewinder enables high-fidelity DNA assembly by separating the information that guides assembly from the final assembled sequence.

Nature research paper: Construction of complex and diverse DNA sequences using DNA three-way junctions

go.nature.com/4qu2ExZ

Not just biomedicine! Chemistry, physics, computer science, climate? Both ARC and NHMRC massively under funded. Success rates abysmal.

Skeptical of claim re budget increase by 250%? Aus R&D expenditure (%GDP) is stagnant, significantly below OECD average.

Inflation. Rising costs. USA exit. ?!?

First steps toward magnetic-elution affinity chromatographies, easily pattern surfaces, microfluidics, also probably cheaper than mag beads for robotic pipelines. Keen to see the data!

The latest research from our team is out in Cell @cp-cell.bsky.social! We used #cryo-EM, #imaging, #BRET, and #biosensors targeted to the lysosome to understand how the KICSTOR-GATOR1 complex turns off #mTORC1 in response to low levels of amino acids.

Excited to share our latest research, out today in Cell (@cellpress.bsky.social)

Using cryo-EM and cell-based studies, we reveal how large nutrient sensing protein complexes form at the lysosomal membrane to turn off mTORC1 and halt anabolic signalling.

Read the paper here: doi.org/10.1016/j.ce...

Most researchers miss out on innovation grants while medical fund sits on $25b Nine in 10 Australian researchers had their “ideas grant” applications rejected last year, even as Australia’s medical investment fund sits on $5 billion more than it was designed to hold.

The government can’t claim that medical research is a priority while failing to treat it as one. Nine in 10 leading researchers in Australia are missing out on government support for world‑class proposals, leaving exceptional talent uncertain about their future.

You're very kind to say so! Thanks Brett

Structure of the lysosomal KICSTOR-GATOR1-SAMTOR nutrient-sensing supercomplex This study reveals how KICSTOR and GATOR1 assemble into a crescent-shaped lysosomal complex that regulates mTORC1 during amino acid starvation and how SAMTOR binds KICSTOR in an S-adenosylmethionine-incompatible conformation to couple methionine sensing to mTORC1 regulation.

Now online! Structure of the lysosomal KICSTOR-GATOR1-SAMTOR nutrient-sensing supercomplex

Check out the manuscript for an exciting and unexpected finding!

👀

Here's an overview of the structure...

doi.org/10.1016/j.ce...

This figure captures how challenging the #cryoEM analysis was, but it doesn't capture the challenges we had to solve in molecular biology, biochemistry, purification, and functional testing. Every step was hard! So glad to see it available.

Thrilled to share our latest work @luptoncj.bsky.social @drellisdon.bsky.social @drmlhalls.bsky.social.

Structure of the lysosomal KICSTOR-GATOR1-SAMTOR nutrient-sensing supercomplex.

🧪🔬#cryoEM
Now online @cellpress.bsky.social.

doi.org/10.1016/j.ce...

AMAZING.

Amazing - thank you both - the annotation spread sheet on zenodo is exactly what I was looking for... very helpful resource :) 🙏

ODD & EVN if possible, I want to run isonet2 🙏

I kind of want to use the ones from the isonet2 paper, but again I'd rather not download and screen through them all just for three or four examples.

Crowd sourcing request!! I'm trialling some dev software and I'm looking for nice public tomograms to use as case studies. Downloading all tomos and looking for good examples is tedious. Can anyone recommend specific tomos from EMPIAR/CZI that have multiple STA targets?

#teamtomo #cryoEM

CryoEM folks, If you want to handle huge STAR file efficiently in Python, please try starfile-rs!

github.com/hanjinliu/star…

Reading a motion data of a particle from a 15 MB file is just like this ↓🤩

Interesting discussion. Pros/cons of PDFs vs digital objects.

As our standards increase, the burden of evidence will so too. These monster figs are a consequence. Why are we still held to prehistoric paper press page limits? Why not have as many ext. data figs as necessary? A PDF can be 100 pgs.

IsoNet2 determines cellular structures at submolecular resolution without averaging www.biorxiv.org/content/10.64898/2025.12.09.693325v1 #cryoEM