Cecilia Zaza

👏Congrats to the whole team! Megan D. Joseph, Olivia LP Dalby, Rhian Walther, Karol Kołątaj, German Chiarelli, @fpichaud.bsky.social, Guillermo Acuna and Sabrina Simoncelli.

Special thanks to @healthcare.nikon.com and @lmcb-ucl.bsky.social Imaging facility for their support.

30.05.2025 15:17 — 👍 8    🔁 1    💬 1    📌 0

🔬 We believe this advancement will unlock new biological questions at scales and resolutions previously unattainable.
🧬 What systems would you explore with this level of detail?

30.05.2025 15:14 — 👍 3    🔁 0    💬 1    📌 0

✨ And yes—we go 3D.
We achieve:
✅ sub-10 nm localization precision at 9 μm depth in whole cells 🦠
✅ ~12 nm precision in complex tissues (like the Drosophila eye 👁)
Think about that: near-molecular detail, deep inside tissues.

30.05.2025 15:12 — 👍 2    🔁 0    💬 1    📌 0

✨ We push further:
🌈 Multicolour imaging and 🎯 #RESI = repetitive imaging → ultra-high resolution.
This opens complex multiplexed experiments inside cells, where precision matters.

30.05.2025 15:12 — 👍 2    🔁 0    💬 1    📌 0

✅ On a commercial microscope, we achieve sub-10 nm resolution on DNA origami across a 76 x76 μm2 field of view!
✅ We apply this in cells to resolve nuclear pore complex protein pairs inside the cellular context.
This is true protein-level imaging, across large scales.

30.05.2025 15:11 — 👍 2    🔁 0    💬 1    📌 0

Super-resolution imaging in whole cells and tissues via DNA-PAINT on a spinning disk confocal with optical photon reassignment - Nature Communications Zaza and colleagues demonstrate that DNA-PAINT on a spinning disk confocal microscope with optical photon reassignment enables high-resolution imaging across large fields and imaging depths, resolving...

Can we break past the usual limits of #SuperResolution #Microscopy—achieving deep-imaging, high-res, large FOV imaging without the trade-offs?
🧬🔬 YES: with #DNA_PAINT + Spinning Disk Confocal + Optical Photon Reassignment (#SDC-OPR)!
📄 www.nature.com/articles/s41... @natcomms.nature.com!
🧵👇

30.05.2025 15:10 — 👍 31    🔁 9    💬 1    📌 0

Super-resolution imaging in whole cells and tissues via DNA-PAINT on a spinning disk confocal with optical photon reassignment - Nature Communications Zaza and colleagues demonstrate that DNA-PAINT on a spinning disk confocal microscope with optical photon reassignment enables high-resolution imaging across large fields and imaging depths, resolving...

Great new DNA/Q-Paint method published by our @ucl colleague Sabrina Simoncelli - lucky to have been part of it! Check out the Ecadherin and collagenIV imaging in the fly retina at up to 15micron depth www.nature.com/articles/s41...

29.05.2025 19:10 — 👍 28    🔁 11    💬 0    📌 0