High-resolution #CryoET provides ‘compelling’ evidence for the true 3D architecture of glutamatergic synapses: a complex ‘synaptoplasm’ rather than the textbook postsynaptic density.
buff.ly/hOYKjHH

How does hunger spur us to consume? @columbiauniversity.bsky.social scientists have found a brain area that drives mice to consume sweets, fats, salt & food. This finding may inform novel treatments for both overeating and undereating:
zuckermaninstitute.columbia.edu/brain-dial-c...

Progress in the development of an ultrafast pulsed ponderomotive phase plate for cryo-electron tomography pubmed.ncbi.nlm.nih.gov/40896196/ #cryoEM

Brooklyn at sunset

Our latest paper on the development of an #ultrafast #laser #PhasePlate for #cryoET @fitzpatricklab.bsky.social @zuckermanbrain.bsky.social

doi.org/10.1063/4.0000770

Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators.

www.nature.com/articles/s41...

Contrast by electron microscopy in thick biological specimens pubmed.ncbi.nlm.nih.gov/40857101/ #cryoEM

Optimal tilt-increment for cryo-ET www.biorxiv.org/content/10.1101/2025.08.20.671201v1 #cryoEM

Fourier-based multiple-slice reconstruction in cryo-electron tomography pubmed.ncbi.nlm.nih.gov/40834650/ #cryoEM

A massive genetic study in @Nature just identified why chronic pain hits some people harder than others.

They discovered the first neuronal “pain transporter” - and it could unlock entirely new ways to treat pain. 🧵

Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug Nature Structural & Molecular Biology, Published online: 18 August 2025; doi:10.1038/s41594-025-01632-3Integrated structural biology approach leveraging in situ cryo-electron tomography reveals molecular details of the native malarial translation elongation cycle and its perturbation by cabamiquine, a potent antimalarial drug candidate.

ICYMI: New online: Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug

New "Mindscapes" exhibit @zuckermanbrain.bsky.social‬ featuring #cryoEM structures of #TMEM106B fibrils (doi.org/10.1016/j.cell.2022.02.026) and the ligand-bound human sweet receptor (doi.org/10.1016/j.cell.2025.04.021) from the Fitzpatrick (www.fitzpatrick-lab.org) and Zuker (www.zukerlab.com) labs

Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug Nature Structural & Molecular Biology - Integrated structural biology approach leveraging in situ cryo-electron tomography reveals molecular details of the native malarial translation...

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH

A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

Flexibility can be frustrating when processing a #cryoEM dataset. If your target has a wobbly domain, check out our new case study on FaNaC1 for continuous heterogeneity tips in #CryoSPARC!

guide.cryosparc.com/processing-d...

What's new in biology: summer 2025 The first gonorrhea vaccination program, contact lenses that see infrared light, the protein behind sweet tastes, a baby cured with gene therapy, and more

New post! Our summer edition of What's new in biology:

The first gonorrhea vaccination program, contact lenses that see infrared light, the protein behind sweet tastes, baby cured with gene therapy, gene-editing breakthroughs & new cholesterol drugs.

Written by me and @nikomccarty.bsky.social

A practical look at cryo-electron tomography image processing: Key considerations for new biological discoveries Cryo-electron tomography (cryo-ET) enables 3D visualization of complex biological environments without the need for purification, thereby preserving t…

Happy to say that my Current Opinion in Structural Biology article came out today. I hope it helps to guide to you in using cryo-ET to make new biological discoveries!

www.sciencedirect.com/science/arti...

Forward-looking preview by Bin Wu to our latest paper in Structure by @andrewnchang.bsky.social highlighting the power of #AI in #cryoEM of patient-derived samples:

tinyurl.com/tu2fyvme

Paper:

tinyurl.com/3cycffzm

Many thanks to @thorstenwagner.bsky.social @raunser-lab.bsky.social for #crYOLO

Ever wonder why SGD, which works so well for ab initio cryo-EM, never seems to get to high resolution on its own? Well, we have the answer: conditioning!

Proud to share our latest paper. doi.org/10.1016/j.cr...

Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.

Structural biology in tissue is well and truly here!

@rosfrankinst.bsky.social

New preprint from Daniel Du @fitzpatricklab.bsky.social shows #femtosecond light pulses can lens electron beams, promising higher contrast in #cryoET and better aberration correction in #cryoEM

tinyurl.com/257sw7j9

Experiments made possible by support from @cziscience.bsky.social #ImagingTheFuture

An approach for coherent periodogram averaging of tilt-series data for improved contrast transfer function estimation

👉 buff.ly/hDgWZMA

#cryoEM #StructuralBiology #CryoET

Structural basis of human uromodulin filament networks in uropathogen capture pubmed.ncbi.nlm.nih.gov/40345203/ #cryoEM

>20 years ago, Zuker et al. discovered the mammalian sweet taste receptors

Now, an exciting collaboration between our labs reveals the #cryoEM structure of the human sweet receptor-key to sweet taste perception-online @cp-cell.bsky.social!
www.sciencedirect.com/science/article/pii/S0092867425004568

Hey #TeamTomo,
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!

tomoguide.github.io

Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪

Structural basis of lipid transfer by a bridge-like lipid-transfer protein - Nature The LPD-3 complex structure reveals protein–lipid interactions that suggest a model for how the native LPD-3 complex mediates bulk lipid transport and provides a foundation for mechanistic studies of bridge-like lipid-transport proteins.

Nature research paper: Structural basis of lipid transfer by a bridge-like lipid-transfer protein

https://go.nature.com/3Gn657r

Molecular basis of human GABA transporter 3 inhibition Nature Communications - GABA transporters maintain GABA homeostasis and regulate synaptic signaling. Here, the authors determined the cryoEM structures of GABA transporter 3 (GAT3) in its apo form...

I'm excited to share our lab's newest publication on the structure and pharmacology of human GABA transporter 3 @natcomms.nature.com. Much to our surprise, GAT3 adopts an inward-open conformation in complex with the (moderately) selective antagonist SNAP-5114. 💊 🧪 #CryoEM #OpenAccess rdcu.be/ei2KY

Figure 4. MemBrain v2 end-to-end workflow detects periodic phycobilisome organization. A: Raw tomogram slice of EMD-31244. B: Out-of-the-box MemBrain-seg segmentation (light blue). C: A single membrane instance can be visualized in Surforama and manually annotated with GT phycobilisome positions (magenta). D: MemBrain-pick localizes particles (trained with data from C) on all membranes in the tomogram. E: MemBrain-stats computes Ripley’s O statistic using the positions from D with a bin size of 5nm. The distance between peaks (35 nm) was measured to estimate chain unit spacings.

We have updated our #MemBrain v2 preprint with a lot more details about the MemBrain-pick and MemBrain-stats modules, as well as some application examples!

Stay tuned for the upcoming thread by lead author @lorenzlamm.bsky.social! 🧠🧵

#CryoET #TeamTomo
www.biorxiv.org/content/10.1...

3/ Awardee @cmholab.bsky.social tackles the complex challenges of protein sequencing by leveraging microbial molecular machinery. Her work can revolutionize how we study protein function/structure & our understanding of biology🤯.
🥳Congrats Dr. Ho! @columbiauniversity.bsky.social
🙏#HFScout Ellen Yeh

Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...

@wehi-research.bsky.social @komanderlab.bsky.social

@science.org Cryo-EM reveals mechanisms of natural RNA multivalency | Science www.science.org/doi/10.1126/...
#RNAsky #RNAbiology #Cryo-EM