Siggy Nachtergaele

Pre-print #2 from the lab this year! We took a closer look at the m1A methyltransferase TRMT61B (a super interesting enzyme that targets multiple types of RNA), and explored location-dependent effects of TRMT61B-mediated m1A on mRNA translation. Led by very talented grad student, Dorthy Fang!

A CRISPR activation screen reveals a cilia disassembly pathway mutated in focal cortical dysplasia A gain-of-function screen uncovers a cilia disassembly pathway linked to genes somatically mutated in neurodevelopmental disease.

Excited to share our latest work on a new cilia disassembly pathway and a link between this pathway and the neurological disorder focal cortical dysplasia: www.science.org/doi/10.1126/...
Brief summary: we used a genome-wide CRISPRa GOF screen to identify negative regulators of ciliary signaling...

Don't forget to join us tomorrow for the RNA Club!

🎟️ Don’t miss your chance to add a new stamp to your RNA Club card and keep the collection growing

Congrats to Chia-Ling Chou, Emmanuel Saliba, Anna Marie Pyle, Li-Tao Guo and co-authors on demonstrating how single-cell RNA-Seq with UltraMarathonRT® reveals unexpected transcriptomic diversity.

🔗👇

@emmanuel-saliba.bsky.social @litaoguo.bsky.social

#singlecell #scRNAseq #RNA #RNAresearch #RNASky

Flyer for the Yale RNA Science and Medicine 2025 Retreat. The title reads 'YALE CENTER FOR RNA SCIENCE AND MEDICINE' at the top. The event, named 'The 2025 Retreat,' will be held on November 7th at Yale's West Campus. Important deadlines are listed: trainee talk abstracts due by September 26th, and registrations and poster abstracts due by October 17th. The background is purple with text in white and dark purple. The bottom of the flyer features four molecular structures, likely representing RNA. A QR code is present on the right side of the flyer, likely for registration or event-related information

Save the Date!
2025 Yale RNA Center for Science and Medicine Retreat on Nov 7 @Grace Hopper Memorial Auditorium, West Campus

Deadlines:
Registration: Oct 17
Talk Abstracts: Sept 26
Poster Abstracts: Oct 17

👉Registration yalesurvey.ca1.qualtrics.com/jfe/form/SV_...
or using the QR code in the image

This work highlights how detailed, transcript-level dissection of RNA modification-driven regulation can expose unexpected, yet clinically actionable mechanisms. Big shoutouts to Emily in the lab for spearheading the effort, and to the NIH and Sontag Foundations for $$$ support!

Blocking m6A installation by inhibiting METTL3 restored chemosensitivity by diminishing expression of transcripts associated with temozolomide resistance, including MGMT, a DNA repair enzyme and critical prognostic marker of poor therapy response in glioblastoma.

We found that m6A promotes therapy resistance by stabilizing a specific subset of transcripts which are upregulated in glioma cells with acquired resistance to temozolomide.

Using a combination of:
✅ Chemoresistant glioblastoma cell culture models
✅ RNA-seq
✅ m6A-eCLIP
we explored how m6A shapes transcriptomic reprogramming as glioblastoma cells adapt to chemotherapy.

N6-methyladenosine promotes temozolomide resistance through non-canonical regulation of mRNA stability in glioblastoma cells N6-methyladenosine (m6A) is a critical regulator of mRNA processing and function, impacting nearly every step of the mRNA lifecycle. Changes in m6A status have been implicated in many different types ...

Thrilled to share a new preprint from the lab! We investigated how m6A regulates the transcriptome in chemoresistant glioblastoma through unexpected mechanisms, and the potential therapeutic applications of this discovery.
Check out the preprint here ⬇️
www.biorxiv.org/content/10.1...

Yale Study: Potential Treatment Pathway Found for Rare and Aggressive Leukemia A Yale study published Aug. 28 on the cover of the journal Blood explains basic scientific discoveries that could lead to innovative therapies for acute myeloid

Excited to share groundbreaking research from our members!

Researchers, including Dr. Stephanie Halene & Dr. Diane Krause, discovered a potential treatment pathway for aggressive leukemia, making it a possible target for the development of new therapies
Read more:
medicine.yale.edu/news-article...

Flyer for the Yale RNA Club, September 2025. The event is on Tuesday, September 9, 2025 at Bass Center 305, Yale Science Building. Breakfast and coffee at 9:00 AM, talks at 9:15 AM. Speakers: Clara Wang (Nachtergaele Lab) – “Investigating the role of the 18S rRNA m6A methyltransferase METTL5 in translation and metabolism”; and Xiaolin Niu (Ke Lab) – “Mechanistic insights into RNA targeting and cleavage by R-IscB and Cas13d.” Contact: Srikar Gopinath (srikar.gopinath@yale.edu). Flyer includes Yale RNA Center branding with autumn-themed colors.

✨ RNA Club is back! ✨

We’ve missed you and the great science talks!

Kick off the semester with us on Tuesday, September 9 at 9:00 AM in Bass Center 305, Yale Science Building.

Enjoy bagels & coffee plus exciting talks from Clara Wang and Dr. Xiaolin Niu.

Don’t miss it! 🧬

🔬 Join us LIVE on Zoom this Wednesday, June 11, at 4 PM ET!
We’re excited to co-host the RNA Collaborative Seminar Series featuring
🎙️ Dr. @siggyn5.bsky.social (Yale RNA Center)
🎙️ Dr. Alex Wesselhoeft (HMS Initiative for RNA Medicine)

Registration 👉 lnkd.in/e-9Huhzd

RNAConnect presents at RNA Society Meeting 2025

We're looking forward to another productive day at #RNA25! Join us Friday morning at 7:45am for Breakfast & Breakthroughs plus engaging poster sessions in the evening featuring the latest innovations with UltraMarathonRT.

#RNA2025 #RNAresearch #Transcriptomics #RNASky

RNAConnect's sponsored breakfast session at RNA 2025

Join Drs. Jason Underwood & Anthony Mustoe at RNAConnect's ‘Breakfast & Breakthroughs’ during RNA 2025!

🗓 Fri, May 30 | 7:45–8:45 AM
📍Town and Country Ballroom

Seats are limited so register today! 🔗https://bit.ly/43qC0vU

#RNA2025 #RNA25 #RNASociety #RNAConnect #RNA #RNABiology #RNASky

🌸 Spring RNA vibes at Yale 🌸

Join us for the Yale RNA Club on
📅 Tuesday, April 8
🕛 9:00 AM
📍Cohen Auditorium (Neison Irving Harris Building, YSM)

Breakfast+two great talks on stem cells and leukemia.

Details in the flyer 👇

✨Hey Postdocs and ARS!
Our social event is happening this Monday🎉

To help us plan food and drinks, please use the sign-up link sent through the institutional mailing list by the end of today
Not on the list? Comment below to make sure you get the info

Let’s start the week with new and good company

🌟 Postdocs & ARSs🌟

New at Yale or looking to strengthen your connections?
Join us for an informal Peer Support Program social on

📅Monday, March 24, 2025
🕠4:30–6:30 PM
📍Rose Garden🌹Sterling Hall of Medicine I-Wing, 1st floor 🌹

Looking forward to seeing you there!

More info on institutional email

Incredible science possibilities why I became a biologist

biologylife.substack.com/p/incredible...

So, for those of us who don’t know how medical research funding works (I myself did not), this nice researcher broke it down for me.

💖RNA lovers, this one’s for you!💖
RNA Club: Valentine’s Day edition

Hear fantastic talks from Nachtergaele and Breaker labs, and kick off the morning with fresh, heart-shaped waffles 🧇❤️

📅Tuesday, Feb 11 | 🕢 8:45 AM| 📍 BASS 305

Spread the word, bring a friend, and share your RNA love💕