Ombeline Rossier

Please RT:
We have an opening for a junior group leader position in „Phage Biology & Biotechnology“.
www.fz-juelich.de/de/karriere/...

Interested candidates are encouraged to contact me via email for further details.

@spp2330.bsky.social; @mibinet.bsky.social

Unannotated translation products are widespread in model E. coli Genomes contain orders of magnitude more open reading frames (ORFs) than known protein coding genes, and recent work suggests there may be unannotated proteins present in even the best studied organisms. To address this gap, we used a high throughput reverse genetic toolkit to construct precise C-terminal fusions of a reporter (and control) to >120,000 ORFs in model E. coli . We found hundreds of unannotated significant hits, and individually detected >50 novel polypeptides by western blot, including ORFs within tRNA loci. Many ORFs overlap annotated genes in the sense orientation, and we found these are likely chimeric polypeptides produced by ribosomal frameshifting. Using degron based knockdowns, we identified unannotated proteins that have putative fitness effects, and we found a novel small protein that displays phenotypes consistent with a role in the mRNA degradosome. The observation of a range of unannotated translation products should lead to better annotation and understanding of the bacterial domain of life and motivates the continued exploration of genomes broadly. ### Competing Interest Statement The authors have declared no competing interest.

Unannotated translation products are widespread in model E. coli | bioRxiv https://www.biorxiv.org/content/10.1101/2025.09.25.678689v1?rss=1

Naiavirus: an enveloped giant virus with a pleomorphic, flexible tail - Nature Communications Rodrigues and Queiroz et al. report the discovery of Naiavirus, the largest enveloped virus, isolated from a Brazil biome. With an oval capsid and flexible tail, its giant genome holds many novel gene...

Naiavirus: an enveloped giant virus with a pleomorphic, flexible tail
nature.com/articles/s41467-025-63463-6

The discovery of a Legionella phage explains a key determinant of human disease The majority of Legionnaires’ disease is linked to phage-directed, coincidental selection for virulence in the environment.

The discovery of a Legionella phage explains a key determinant of human disease | Science Advances www.science.org/doi/10.1126/...

#phagesky #microsky

Kiwa is a membrane-embedded defense supercomplex activated at phage attachment sites Zhang, Todeschini, and Wu et al. show that the bacterial defense system Kiwa senses phage attachment at the membrane and assembles a transmembrane complex that halts infection by blocking phage DNA re...

Thrilled to see our Kiwa story out today! A membrane-associated supercomplex that senses infection and blocks replication and transcription.

www.cell.com/cell/fulltex...

Huge congratulations to Yi and Zhiying for bringing it home, to Thomas for starting us off, and to all the collaborators.

A two year post-doctoral position at the BacterialGenome Plasticity Unit starting October 1st - Research

A 2-year postdoc position in my lab, under the supervision of P-Alex Kaminski, on Z-DNA phages and the benefit given by ZTGC DNA and the replication machinery, with attempt to create a Z based minireplicon
research.pasteur.fr/en/job/a-two...

Nuclease-NTPase systems use shared molecular features to control bacterial anti-phage defense Bacteria encode an enormous diversity of defense systems including restriction-modification and CRISPR-Cas that cleave nucleic acid to protect against phage infection. Bioinformatic analyses demonstra...

Starting the lab Bluesky account to share a preprint from @aragucci.bsky.social and @sadieantine.bsky.social‬ that reveals molecular principles shared across diverse nuclease-NTPase anti-phage defense systems in bacterial immunity (1/7)

www.biorxiv.org/content/10.1...

🎓 Impact de l’IA : transformation des pratiques pédagogiques et des formations à l'Université Paris-Saclay

📅 Mardi 23 septembre 2025 – 9h30 à 20h
📍 Théâtre Rousseau, CentraleSupélec - 3 rue Joliot Curie, 91190 Gif-sur-Yvette

@dataiainstitute.bsky.social

tinyurl.com/nhb7kpmk

Beautiful cover from
www.nature.com/articles/s41...

1/10 New pre-print(s) from the Sternberg Lab in collaboration with Leifu Chang's Lab! We uncover the unprecedented molecular mechanism of CRISPR-Cas12f-like proteins, which drive RNA-guided transcription independently of canonical promoter motifs.
Full story here:
www.biorxiv.org/content/10.1...

Bravo David ! Félicitations

#MicroSky

West African–South American pandemic Vibrio cholerae encodes multiple distinct phage defence systems - Nature Microbiology The West African–South American lineage of Vibrio cholerae contains multiple distinct anti-phage defence systems that provide resistance to various phage families, including vibriophage ICP1, a key pr...

Exciting news!! Our latest paper is out in Nat. Microbiol. @natmicrobiol.nature.com

We show that a sub-lineage of 7th pandemic V. cholerae has acquired mobile genetic elements packed with phage defense systems—rendering it multi-phage resistant 😳 ..... 1/3

www.nature.com/articles/s41...

Please add me as well. Thanks!

Associate Senior Lecturer (DDLS fellow) in data driven epidemiology and biology of infection Lund is strategically positioned close to the continent, hosting major national and European research infrastructures, such as MAX.IV Laboratory and the European Spallation Source (ESS). In the field

Bioinformatics junior group leader position in Lund, specialising in infection and/or epidemiology. These are really nice positions with generous support. Please spread the word to anyone who might be interested in becoming our colleague! lu.varbi.com/en/what:job/...

We support our American colleagues #StandUpForSciences #solidarity #Paris

Only 8 days left to apply as a group leader in the department of Virology @i2bcparissaclay.bsky.social
Come join us to work on viruses and their prokaryotic or eukaryotic hosts.

Sustained in situ protein production and release in the mammalian gut by an engineered bacteriophage - Nature Biotechnology Biologics are delivered to the gut using phage that infects resident commensal bacteria.

Sustained in situ protein production and release in the mammalian gut by an engineered bacteriophage go.nature.com/4jWgRAW

Unveiling conserved HIV-1 open reading frames encoding T cell antigens using ribosome profiling - Nature Communications Here, using ribosomal profiling, the authors characterize the translatome of HIV-1 revealing tens of alternative open reading frames (ARF) that encode conserved viral antigens and show that ARF-derive...

I am very pleased to share with you this fantastic work done in in collaboration with Arnaud Moris’steam. It has been a very pleasant collaboration and a fantastic project done at the #I2BC. #microproteine #riboseq #HIV www.nature.com/articles/s41...

The Institute for Integrative Biology of the Cell (I2BC) is recruiting new group leaders interested in viruses of prokaryotic or eukaryotic cells. Located in France, near Paris.
Application deadline : March 15th, 2025
www.i2bc.paris-saclay.fr/wp-content/uploads/2025/01/I2BC-Virology-call_2025.pdf

The Institute for Integrative Biology of the Cell (I2BC) is seeking to recruit new group leaders to join the Virology Department.
The application deadline is March 15th, 2025
www.i2bc.paris-saclay.fr
www.i2bc.paris-saclay.fr/wp-content/uploads/2025/01/I2BC-Virology-call_2025.pdf

Check out this bombshell publication by Michael Fischbach @mfgrp.bsky.social and coworkers @stanford-chemh.bsky.social @stanfordmedicine.bsky.social, imagine a cancer immune therapy/vaccine where treatment begins and ends with a painless topical skin treatment! 🤯

www.nature.com/articles/s41...

MICROBIOLOGISTS 🦠 🧫

You know you’re STELLAR when you earn an -ELLA

Alberto Barton 🇵🇪
Jules Bordet 🇧🇪
Sir David Bruce 🏴󠁧󠁢󠁳󠁣󠁴󠁿
Edward Francis 🇺🇸
Elizabeth King 🇺🇸
Edwin Klebs 🇩🇪
Victor Morax 🇨🇭
André Prévot 🇫🇷
Daniel Salmon 🇺🇸
Kiyoshi Shiga 🇯🇵

#IDSky #MedSky #MicroSky

Honouring Esther Lederberg’s outstanding work on bacterial gene regulation, recombination and exchange, DSMZ researchers named the strain Streptomyces lederbergiae (DSM 44399) after her.

#herstory #WomenInScience #microbiology #genetics

🚨 I am super happy to announce that our analysis of how the structure of protein-RNA interfaces evolves is now published (early access) at @plos.bsky.social Computational Biology:
doi.org/10.1371/jour...
🧬🧶

A blueprint for broadly effective bacteriophage-antibiotic cocktails against bacterial infections - Nature Communications The application of phage therapy for multidrug-resistant infections is mainly limited to personalized therapy due to the narrow host range of individual phages. Here, Kim et al. identify groups of pha...

www.nature.com/articles/s41...

Diverse phage defence systems define West African South American pandemic Vibrio cholerae Our understanding of the factors underlying the evolutionary success of different lineages of pandemic Vibrio cholerae remains incomplete. Interestingly, two unique genetic signatures define the West ...

Exciting times! Our latest preprint is now live 🤩!
After nearly 16 yrs of running my lab, I truly believe this is one of the most significant contributions we've made. It offers fresh hypotheses about how pandemic Vibrio cholerae spreads globally. Let’s dive in!🧵..1/n
www.biorxiv.org/content/10.1...

Our Big Fantastic Virus Database (BFVD) is now published NAR! It contains protein structure predictions of major viral clades, enhanced by petabase-scale homology search and it's explorable on the web.
🌐 bfvd.foldseek.com
💾 bfvd.steineggerlab.workers.dev
📄 academic.oup.com/nar/advance-...

Get ready to dance, our paper – Phage DisCo: targeted discovery of bacteriophages by co-culture – has been pre-printed! 🪩 This has been a really fun project to work on with @sianowen.bsky.social, @baym.lol, @nquinoneso.bsky.social, and our two talented undergrads Kesther and Carmen!

The PIWI-interacting protein Gtsf1 controls the selective degradation of small RNAs in Paramecium Abstract. Ciliates undergo developmentally programmed genome elimination, in which small RNAs direct the removal of transposable elements (TEs) during the

doi.org/10.1093/nar/...
Glad that our lab contributed to this collaborative work with the Duharcourt and Nowak teams.
#paramecium #sRNA #GenomeBiology #I2BC #ParisSaclay