Construction of antibiotic-free riboflavin producer in Escherichia coli by metabolic engineering strategies with a plasmid stabilization system Riboflavin, an important vitamin utilized in pharmaceutical products and as a feed additive, is mainly produced by metabolically engineered bacterial …

A plasmid stabilization system offers a way to reduce antibiotic reliance in riboflavin bacterial production. Learn more about the solution 👇 👇
doi.org/10.1016/j.sy...

FIND YOUR PLASMID - SEVA plasmids - Standard European Vector Architecture Canonical SEVA plasmids that use Golden Gate assembly to build complex genetic circuits. Find your canonical plasmid In the dropdown menu below, you can select a combination of choice for your…

🎄🦠You don’t know what to give your lab team for Christmas this year yet? What about a customised plasmid perfect for your research? Have a look at our #SEVAplasmids database! 👉 seva-plasmids.com/find-your-pl...

💡A ”shred of light” on contaminants detection! A study shows promising combined biosensors for a more effective and rapid detection of toxins and contaminants. Take a look 👉https://doi.org/10.1016/j.bios.2025.117369

FIND YOUR PLASMID - SEVA plasmids - Standard European Vector Architecture Canonical SEVA plasmids that use Golden Gate assembly to build complex genetic circuits. Find your canonical plasmid In the dropdown menu below, you can select a combination of choice for your…

📖Have you used our #SEVAplasmids database selector? Consult our glossary for Acronyms and Antibiotics abbreviations to easily filter specific features.
👉https://seva-plasmids.com/find-your-plasmid/

Orchestration of secondary metabolite production in Streptomyces species - Nature Biotechnology We developed a universal control system and multitarget optimization framework that enables dynamic and synchronized expression of multiple genes and facilitates the overproduction of specific…

💊 SMARTS biotool for smart #SynBio!
This Streptomyces Multiplexed Artificial Control System boosts production of specific secondary metabolites in this species, which contribute to about half of drugs obtained from microorganisms! Learn more about it: doi.org/10.1038/s415...

FIND YOUR PLASMID - SEVA plasmids - Standard European Vector Architecture Canonical SEVA plasmids that use Golden Gate assembly to build complex genetic circuits. Find your canonical plasmid In the dropdown menu below, you can select a combination of choice for your…

🧬 Looking for the perfect plasmid?
Explore our #SEVAplasmids database. Request any construct for free, or be the first to design one and share it with the community!
Join the standard 👉 seva-plasmids.com/find-your-pl...

💡Glutamate from methanol?

This study confirms that MscS channels in Bacillus methanolicus are key L-glutamate exporters, suggesting a way to unlock the bacterium’s potential for sustainable methanol-based glutamate production.

Take a look 👉 doi.org/10.1111/1751...

Hijacking a bacterial ABC transporter for genetic code expansion - Nature Bacterial ATP-binding cassette (ABC) transporters can be utilized and engineered to transport non-canonical amino acids into Escherichia coli for highly efficient synthesis of proteins with novel…

🚀 New breakthrough! Researchers from the D-CHAB and the TUM Faculty of Natural Sciences have designed bacterial ABC transporters to efficiently import non-canonical amino acids. This achievement is a significant step towards expanding the genetic code. doi.org/10.1038/s415...

FIND YOUR PLASMID - SEVA plasmids - Standard European Vector Architecture Canonical SEVA plasmids that use Golden Gate assembly to build complex genetic circuits. Find your canonical plasmid In the dropdown menu below, you can select a combination of choice for your…

🚀 Take your genetic engineering to the next level!
#SEVA plasmids give you a flexible, plug-and-play platform for your #SynBio designs.
Mix, match, and build the plasmid you need, all in one place 👇
seva-plasmids.com/find-your-pl...

SynBioGPT: A Retrieval-Augmented Large Language Model Platform for AI-Guided Microbial Strain Development Synthetic biology seeks to engineer microbial cell factories for sustainable bioproduction, yet the optimization of these systems is impeded by the complexity of metabolic engineering and the…

🤖 GPT goes #SynBio! Researchers at Chinese Academy of Sciences introduce SynBioGPT v2.0, an AI Large Language Model for SynBio that automates knowledge extraction and strain design. Tested on a 100-question SynBio benchmark, it achieved 98% accuracy!
Take a look: doi.org/10.1101/2025...

Engineered bacteria can degrade five wastewater pollutants at the same time Five gene clusters have been introduced into a single rapidly growing bacterial strain.

🚨 News on bioremediation! A study published in Nature reveals how by genetically modifying the bacterium Vibrio natriegens, it simultaneously degrades five toxic contaminants in industrial wastewater and saline soils. Read the full article: www.nature.com/articles/d41...

Home - SEVA plasmids - Standard European Vector Architecture Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…

👩🔬 Want to team up with us to expand the #SEVA collection?
Contribute your own constructs to our open plasmid repository and help make #SynBio more reproducible for everyone.
✨ Check out our contribution guidelines and join the mission!👇👇
seva-plasmids.com

Generating functional plasmid origins with OriGen While generative artificial intelligence has shown promise for biological design, no computational system has yet created sequences proven capable of replication. Focusing on plasmids as minimal…

Designing replication sequences is challenging in #SynBio. Researchers at the Innovative Genomics Institute present OriGEN, a language model generating functional plasmids beyond wild type, capturing the complex mechanisms of biological replication.
👉 doi.org/10.1101/2025...

Editor’s pick: Constructive Bio - Nature Biotechnology Each year, Nature Biotechnology highlights companies that received sizeable early-stage funding in the previous year. Constructive Bio is using synthetic E. coli and non-canonical amino acids to…

🚀 Meet Constructive Bio! This biotech turns living bacteria into programmable biofactories creating new classes of polymers. At #SEVA, we love seeing initiatives like this driving innovation in #SynBio. Read more 👉 www.nature.com/articles/s41...

Thank you for sharing such promising knowledge!

Home - SEVA plasmids - Standard European Vector Architecture Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…

💭 Imagine #SynBio research where every experiment can be easily replicated and labs collaborate seamlessly. No technical hurdles, just shared progress. That’s our vision at #SEVA! Get your free plasmids and join the movement for #standardisation 👉https://seva-plasmids.com/

Pseudomonas chlororaphis is a promising biofactory, but hard to engineer. A new piece of work has characterised its endogenous CRISPR/Cas I–F system and built a precise editing & CRISPRi toolkit, enabling efficient genetic manipulation. Take a look 👉 pubs.acs.org/doi/10.1021/...

An M13 phagemid toolbox for engineering tuneable DNA communication in bacterial consortia Intercellular communication is essential for distributed genetic circuits operating across cells in multicellular consortia. While diverse signalling molecules have been employed—ranging from quorum…

Looking for efficient ways to program intercellular communication? 🧬
Researchers at Micalis Institute present a phagemid toolbox —with #SEVA-based variants— for DNA delivery and next-gen multicellular circuits.
👉 doi.org/10.1101/2025...

Frontiers | Genetic circuits in synthetic biology: broadening the toolbox of regulatory devices Devices sensing inputs and generating outputs are fundamental regulatory units, and as such are the basis of more complex regulatory networks. We provide an ...

The evolution of #SynBio advances with new regulatory toolboxes 🧬
This review explores devices from switches & logic gates to memory modules, powering circuits for bioproduction, therapeutics & biosafety — maybe you’ll find the ideal one for your research 👉 doi.org/10.3389/fsyb...

Evaluation of vectors for gene expression in Pseudovibrio marine bacteria | Applied and Environmental Microbiology The genus Pseudovibrio of α-Proteobacteria has consistently been isolated from marine sponges and other marine organisms such as corals and algae. Pseudovibrio bacteria are a source of antibiotics…

🌊 Marine bacteria like Pseudovibrio hold enormous ecological & biotech potential, still largely unexplored.✨ Discover the new SEVA-based genetic toolbox developed by researchers at the University of Illinois Chicago — opening ways to study their biology & new applications👉 doi.org/10.1128/aem....

Home - SEVA plasmids - Standard European Vector Architecture Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…

What makes a SEVA plasmid unique?

It’s built around 3 fundamental modules:
1️⃣ Origin of replication
2️⃣ Selection marker
3️⃣ Cargo

With this open standard, you can design predictable and efficient genetic systems in bacteria and yeast.
👉 Learn more here:
seva-plasmids.com

How many plasmids can bacteria carry? A synthetic biology perspective | Open Biology Plasmids are pinnacle tools in synthetic biology and other biotechnological applications. They serve as the simplest approach to introduce recombinant DNA, which is then transcribed into RNA that…

🧬 How many plasmids can a single bacterium carry?

That’s the question Cholpisit Kiattisewee explores in his review on multi-plasmid systems and their impact on #SynBio. And yes, #SEVA is part of the debate 👀
👉 Read the full article: doi.org/10.1098/rsob...

Home - SEVA plasmids - Standard European Vector Architecture Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…

⚠️ Hold on! Don’t scroll!
Still don't know the basic design of a SEVA plasmid?

🧩 Combine modular parts and get your own standard plasmid from @SEVAplasmids for free.

Find your match here 👇
seva-plasmids.com

Home - SEVA plasmids - Standard European Vector Architecture Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…

Biology isn’t an exact science — but standardisation helps us get closer to reproducibility and clarity.

That’s why @SEVAplasmids created a universal framework for plasmid vector design. Explore it here 👇
seva-plasmids.com

Constructing synthetic nuclear architectures via transcriptional condensates in a DNA protonucleus Nuclear biomolecular condensates are functional sub-compartments within the cell nucleus. Here, the authors develop a synthetic DNA protonucleus that enables RNA transcription and condensation into diverse...

How do you imagine an artificial DNA nucleus? This study presents a synthetic DNA protonucleus that enables localized RNA transcription and condensation into nuclear-like architectures. A novel tool to investigate real nuclear function. Take a look
👉 doi.org/10.1038/s414...

Simple and robust in vivo engineering of plasmid DNA at any copy number in Escherichia coli A robust in vivo method for engineering plasmid DNA in E. coli using a triple-selection system that works at any copy number, enabling fast, scalable construction of standardized plasmids for synthetic...

🚀 #SEVA proves its potential once again!

@norholm.bsky.social's lab created a new plasmid recombination system in E. coli using SEVAplasmids. Compatible with any copy number, they expand the standardised toolbox for #SynBio 👉 doi.org/10.1038/s420...

Home - SEVA plasmids - Standard European Vector Architecture Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…

⏳ Stop wasting hours searching for the perfect plasmid for your experiment.

👉 Discover @SEVAplasmids — a community-driven, non-profit collection of standardised plasmids.

🔬 Open, reliable, and ready for your research.

Check it out here 👇
seva-plasmids.com

Scaling laws of bacterial and archaeal plasmids - Nature Communications The capacity of a plasmid to express genes is constrained by parameters such as its length and copy number. Here, Maddamsetti et al. present a computational method that enables rapid and accurate…

📰 A computational analysis reveals 3️⃣ scaling laws of plasmids. Longer plasmids have:

1️⃣fewer copies
2️⃣more protein-coding
3️⃣more metabolic genes

And with gradual adoption of chromosome-like features as they become longer. For more details 👉 doi.org/10.1038/s414...

Creating a Genetic Toolbox for the Carbon-Fixing, Nitrogen-Fixing and Dehalogenating Bacterium Xanthobacter autotrophicus Xanthobacter autotrophicus is a metabolically flexible microorganism with two key features: (1) The organism has adapted to grow on a wide variety of carbon sources including CO2, methanol, formate,…

🧬🔧X. autotrophicus, despite its metabolic flexibility and biosynthetic potential, has few genetic tools available. Researchers from Northeastern University introduce a new toolbox for this microbe, enabling optimisation of processes like bioremediation👉https://doi.org/10.1021/acssynbio.4c00455

A framework for complex signal processing via synthetic biological operational amplifiers - Nature Communications Engineering genetic circuits to control gene expression in response to complex signal inputs remains a challenge due to limited precision. This study introduces operational amplifiers that decode…

📢 A study in Nature Communications presents a new framework that integrates scalable orthogonal amplifiers into standardised biological processes, increasing precision, adaptability, and signal-to-noise ratio in complex genetic circuits. Read the full study here👉 doi.org/10.1038/s414...