Constructing synthetic nuclear architectures via transcriptional condensates in a DNA protonucleus Nuclear biomolecular condensates are functional sub-compartments within the cell nucleus. Here, the authors develop a synthetic DNA protonucleus that enables RNA transcription and condensation into diverse...

How do you imagine an artificial DNA nucleus? This study presents a synthetic DNA protonucleus that enables localized RNA transcription and condensation into nuclear-like architectures. A novel tool to investigate real nuclear function. Take a look
👉 doi.org/10.1038/s414...

Simple and robust in vivo engineering of plasmid DNA at any copy number in Escherichia coli A robust in vivo method for engineering plasmid DNA in E. coli using a triple-selection system that works at any copy number, enabling fast, scalable construction of standardized plasmids for synthetic...

🚀 #SEVA proves its potential once again!

@norholm.bsky.social's lab created a new plasmid recombination system in E. coli using SEVAplasmids. Compatible with any copy number, they expand the standardised toolbox for #SynBio 👉 doi.org/10.1038/s420...

Home - SEVA plasmids - Standard European Vector Architecture Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…

⏳ Stop wasting hours searching for the perfect plasmid for your experiment.

👉 Discover @SEVAplasmids — a community-driven, non-profit collection of standardised plasmids.

🔬 Open, reliable, and ready for your research.

Check it out here 👇
seva-plasmids.com

Scaling laws of bacterial and archaeal plasmids - Nature Communications The capacity of a plasmid to express genes is constrained by parameters such as its length and copy number. Here, Maddamsetti et al. present a computational method that enables rapid and accurate…

📰 A computational analysis reveals 3️⃣ scaling laws of plasmids. Longer plasmids have:

1️⃣fewer copies
2️⃣more protein-coding
3️⃣more metabolic genes

And with gradual adoption of chromosome-like features as they become longer. For more details 👉 doi.org/10.1038/s414...

Creating a Genetic Toolbox for the Carbon-Fixing, Nitrogen-Fixing and Dehalogenating Bacterium Xanthobacter autotrophicus Xanthobacter autotrophicus is a metabolically flexible microorganism with two key features: (1) The organism has adapted to grow on a wide variety of carbon sources including CO2, methanol, formate,…

🧬🔧X. autotrophicus, despite its metabolic flexibility and biosynthetic potential, has few genetic tools available. Researchers from Northeastern University introduce a new toolbox for this microbe, enabling optimisation of processes like bioremediation👉https://doi.org/10.1021/acssynbio.4c00455

A framework for complex signal processing via synthetic biological operational amplifiers - Nature Communications Engineering genetic circuits to control gene expression in response to complex signal inputs remains a challenge due to limited precision. This study introduces operational amplifiers that decode…

📢 A study in Nature Communications presents a new framework that integrates scalable orthogonal amplifiers into standardised biological processes, increasing precision, adaptability, and signal-to-noise ratio in complex genetic circuits. Read the full study here👉 doi.org/10.1038/s414...

CONTACT - SEVA plasmids - Standard European Vector Architecture Order plasmids If you wish to order SEVA plasmids, please fill in the form below. We offer this service pro bono so it is free of charge. Large orders ( > 15 plasmids) may experience shipment delays…

With #SEVAplasmids you’re just 2 steps away from your ideal plasmid!

1️⃣ Design your plasmid
2️⃣ Fill in the super simple order form

That’s it —your customised plasmids will be shipped straight to your lab 🚀 at no cost
Start now👉 seva-plasmids.com/contact/

Genetic toolbox for Photorhabdus and Xenorhabdus: pSEVA based heterologous expression systems and CRISPR/Cpf1 based genome editing for rapid natural product profiling - Microbial Cell Factories Background Bacteria of the genus Photorhabdus and Xenorhabdus are motile, Gram-negative bacteria that live in symbiosis with entomopathogenic nematodes. Due to their complex life cycle, they produce…

🧑🤝🧑CRISPR/Cpf1 × pSEVA: a powerhouse combo in #SynBio! German researchers applied this strategy in Photorhabdus & Xenorhabdus, boosting production of high-value compounds like safracin B, a precursor to the anti-cancer drug ET-743. Read here👉 doi.org/10.1186/s129...

RESEARCH | Tom Ellis Lab Tom Ellis Lab - Publications, Resources and PhD Thesis from the Synthetic Genomics and Synthetic Biology Research Group at Imperial College London. A bioengineering and biotechnology research group…

Meet one of our partners: @proftomellis.bsky.social's Lab, advancing genetically modified living materials. They use bacteria and yeast to create innovative biomaterials and were key in expanding SEVA from bacteria to eukaryotes. Find out more 👉https://www.tomellislab.com/our-research

Boosting energy metabolism and biosynthesis in diverse organisms by a common bacterial salvage lipoylation protein - Nature Communications Lipoylated proteins are essential for energy metabolism of both prokaryotes and eukaryotes. Here, the authors showed that the E. coli lipoate-protein ligase A (LplA) can boost the lipoylation of key…

#SynBio never ceases to amaze: a new study has succeeded in increasing energy metabolism in mammalian cells, algae, and fungi through the expression of E. coli LplA, a lipoylation protein. The result? Read here 👉 doi.org/10.1038/s414...

Home - SEVA plasmids - Standard European Vector Architecture Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…

Why choose between standardisation and adaptability when you can have both? With #SEVA, you can mix and match:

✅ Antibiotic resistance marker
✅ Origins of replication
✅ Cargo modules for your experiment

Build your reproducible #SynBio project here👉 seva-plasmids.com

RESEARCH | Tom Ellis Lab Tom Ellis Lab - Publications, Resources and PhD Thesis from the Synthetic Genomics and Synthetic Biology Research Group at Imperial College London. A bioengineering and biotechnology research group…

Meet one of our partners: @proftomellis.bsky.social's Lab, advancing genetically modified living materials. They use bacteria and yeast to create innovative biomaterials and were key in expanding SEVA from bacteria to eukaryotes. Find out more 👉https://www.tomellislab.com/our-research

Bioremediation of complex organic pollutants by engineered Vibrio natriegens - Nature A synthetic biology approach was used to engineer Vibrio natriegens into a strain capable of bioremediating complex organic pollutants in saline wastewater and soils, thereby addressing notable…

🚨 News for marine biosafety: Chinese researchers engineered a Vibrio natriegens strain, capable of degrading five water organic contaminants: biphenyl, phenol, naphthalene, dibenzofuran, and toluene. Find out how they did it 👉https://doi.org/10.1038/s41586-025-08947-7

⏳Looking for fast genome editing systems? Researchers from @uwaterloo.ca & Chinese Academy of Sciences created an all-in-one plasmid CRISPR/Cas9 system for Pseudomonas putida KT2440, enabling a genome edit in less than 1.5 days. Explore the work 👉 doi.org/10.1186/s129...

FIND YOUR PLASMID - SEVA plasmids - Standard European Vector Architecture Canonical SEVA plasmids that use Golden Gate assembly to build complex genetic circuits. Find your canonical plasmid In the dropdown menu below, you can select a combination of choice for your…

3️⃣ 2️⃣ 1️⃣... Not a countdown—it’s the standard code of one of our plasmids!

3️⃣: Cm resistance
2️⃣: RK2 origin of replication
1️⃣: MCS-default cargo

Put them together, and voilà: pSEVA321 plasmid. Simple as that!

Find more standardised plasmids here👉 seva-plasmids.com/find-your-pl...

Designing pathways for bioproducing complex chemicals by combining tools for pathway extraction and ranking - Nature Communications Synthesis of complex molecules requires reactions from multiple pathways operating in balanced subnetworks. Here, the authors report a computational algorithm that combines the strength of…

Creating complete biosynthetic pathways from scratch can be a nightmare. SubNetX is a tool that helps you design and integrate them into whole-cell models, optimising the production of complex biochemical compounds. Discover how it works 👉 doi.org/10.1038/s414...

Could lignin waste inspire the biomanufacturing of the future?
An international team evolved Acinetobacter baylyi to consume multiple lignin-derived aromatics simultaneously, paving the way for circular bio-based production. Read the article:
👉 doi.org/10.1093/jamb...

Modular plasmid design for autonomous multi-protein expression in Escherichia coli - Journal of Biological Engineering Background Molecular and synthetic biology tools enable the design of new-to-nature biological systems, including genetically engineered microorganisms, recombinant proteins, and novel metabolic…

SynBio still needs tools enabling independent & precise co-expression of multiple genes. Researchers at Wrocław University presented a new plasmid set with 4 regulatory systems applied in E.coli, enabling independent induction of up to 3 genes in a single vector. Learn more 👉 doi.org/10.1186/s130...

Home - SEVA plasmids - Standard European Vector Architecture Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…

🧬 Simplify your genetic engineering! #SEVAplasmids offers a growing collection of modular, “plug-and-play” plasmids designed to accelerate #SynBio workflows. Choose, combine, and customise with confidence thanks to our clear standards. 👉 seva-plasmids.com

New #SynBio tool! Juan Nogales' lab presents GENIO, a platform for precise genome integration in Pseudomonas putida inspired by #SEVAplasmids’ modular design. It helps you optimise genetic circuit performance for complex synbio design. Learn more: doi.org/10.1111/1751...

🎯 Looking for a plasmid with specific features? Use our #SEVAplasmids selector to filter by origin, antibiotic resistance, or cargo. 👉 Find your right tool in seconds: seva-plasmids.com/select-a-pla...

Engineered microbial platform confers resistance against heavy metals via phosphomelanin biosynthesis - Nature Communications Heavy metals and plastics are two major environmental pollutants. Here, the authors engineer microbial platforms capable of endogenously biosynthesizing phosphomelanin for simultaneously heavy metals…

🔬 A new microbial platform shows enhanced resistance to heavy metals thanks to phosphomelanin biosynthesis—plus it degrades metallised plastic waste! This could offer promising options for environmental cleanup. Read more 👉 doi.org/10.1038/s414...

BACKBONE MODULES & NOMENCLATURE - SEVA plasmids - Standard European Vector Architecture Plasmid Backbone The colored arrows above the cargo and AbR modules represent the directionality of the transcription flow of that element SEVA Plasmid Nomenclature The figure sketches the expanded…

🧬 Understanding a plasmid’s structure at a glance? Our interactive map makes it possible. Explore SEVA’s modular architecture and its standardised nomenclature.🔍 ORI, markers, cargo… all just one click away 👉 seva-plasmids.com/backbone-mod...

COLLABORATORS & RESOURCES - SEVA plasmids - Standard European Vector Architecture The SEVA commonwealth Laboratory of Dr. Sven Panke. Bioprocess laboratory. ETH. Switzerland. Laboratory of Dr. Nediljko Budisa.

🤝 Meet Lars M. Blank, partner of #SEVAplasmids community. His laboratory works to advance the bioeconomy by exploring microbial metabolism to transform CO₂, biomass, and plastic waste into valuable chemicals. Meet other collaborators: seva-plasmids.com/the-seva-com...

🌱Researchers at the University of Minnesota engineered a strain of Azotobacter vinelandii to release nitrogen as urea. According to their paper, they achieved a 43× higher yield than before. A promising advance for sustainable agriculture and next-gen biofertilisers 👉 doi.org/10.1111/1751...

🌞 Summer break is here!
The activity of @sevaplasmids.bsky.social is paused for the holidays, so plasmid shipments are on hold until September.
🧬 You can still place your orders — we'll get back to you when we return.
See you in September!

📢New for wastewater decontamination. A new study explores how the mobile element IS1071 can speed up pesticide degradation in an on-farm biopurification system.
By mobilising catabolic genes in microbes, it helps detoxify wastewater more efficiently. Read more👉 doi.org/10.1111/1462...

Curious about jumbo phages? Dive into the fascinating world of phage 201ϕ2-1 — discover new insights into its transcription machinery and how it could be harnessed for synthetic biology applications. Sounds exciting? Take a look 👉https://doi.org/10.1111/1751-7915.70037

COLLABORATORS & RESOURCES - SEVA plasmids - Standard European Vector Architecture The SEVA commonwealth Laboratory of Dr. Sven Panke. Bioprocess laboratory. ETH. Switzerland. Laboratory of Dr. Nediljko Budisa.

🤝Meet one of our #SEVAplamids community members: Dr.Jens Kromer’s lab. The group works on cell factories for fuels, chemicals and therapeutics, marine sponges and valorizes photosynthetic, CO₂ and sunlight products. Meet other partners👉 seva-plasmids.com/the-seva-com...

New step in metabolic engineering! A genome editing system for Caldimonas thermodepolymerans develops:

✅ a chassis strain (AI01) with improved transformation efficiency
✅ a mutant (ΔphaC) for polyhydroxyalkanoates synthesis

See how: 👉 doi.org/10.1111/1751...