Danielle Grotjahn

Leopard-EM: an extensible 2D template-matching package to accelerate in situ structural biology In situ cryo-EM coupled with 2D template matching (2DTM) holds the potential to visualize the cellular structureome in context, but further developments are required to make this a reality. We describ...

Now out in full form. Again, a big thanks to #teamtomo and @alisterburt.bsky.social for developing the infrastructure to build this on. journals.iucr.org/d/issues/202...

We will announce a #postdoc opening in January. Cryo-ET and virus replication. If you're interested you can DM or email me already now. More info will follow!

What an honor to see @attychang.bsky.social’s work featured in @jcb.org The Year in Cell Biology 2025 collection! Even more special that it coincides with my first #cellbio2025 conference 🙂

s/o to co-authors @baradlab.com @hamid13r.bsky.social @zidlab.bsky.social

#teamtomo #cellbio25

Prohibitins aren’t hanging out just anywhere in the membrane — they prefer the 💁‍♀️💎✨VIP section💅🪩🥂

Lab all-star @mmedina300kv.bsky.social maps these microdomains using cryo-ET + surface morphometrics.

s/o to #teamtomo #cryoET co-authors
@hamid13r.bsky.social
@attychang.bsky.social
@baradlab.com

Exciting !

Thank you to all of our plenary speakers for sharing your incredible research with us at #ASBMB2025! @nanigrotjahn.bsky.social @ajknights7.bsky.social Michelle Haber and Randal Halfmann. We have loved having you all in Brisbane and hearing about your labs awesome work! 🦠🧬🧪🧫🔬

Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit
www.nexperion.net/semcm2026

What an inspiring few days at the #WEHeraeusSeminar! Great science, lively discussions, and lots of fun with the amazing community of #cryoEM, #StructuralBiology & #Microscopy. Huge thanks to everyone!

We all love colours, especially, when they can be generated automatically! Fantastic talk by Danielle Grotjahn @nanigrotjahn.bsky.social linking method development, structural biology and cell biology! #WEHeraeusSeminar #cryoEM #StructuralBiology #Microscopy @arnemoeller.bsky.social

Excited to travel to Germany 🇩🇪 for the WE-Heraeus-Seminar: Cryo‐EM on the Move: New Applications and Challenges! ❄️🔬🧊

I look forward to hearing what everyone is cooking up to make cryoEM better 📈 faster 🏃‍♀️ stronger 💪 😎

www.we-heraeus-stiftung.de/veranstaltun...

To be considered for this session, please include the following in your submission:

🧫 Topic: Advances in Cell Biology Tools and Technologies
🔬 Subtopic: CryoEM/CryoET
🎤 Format: Minisymposium Talk

Abstract deadline: Sept 3
submissions.mirasmart.com/ASCB2025/Spl...

🚨 #teamtomo!🚨
Did you know there's a dedicated cryoEM/ET Mini-Symposium at the upcoming ASCB/EMBO meeting in Philly (Dec 6–10)??

Ending the week on a high note in SLC with a visit to @uofubiochem.bsky.social after an inspiring M&M 2025 meeting!

It was officially released just today, so you’re only a few hours behind 🙂

Thanks to @baradlab.com for the many conversations about segmentation/morphometrics that helped shape this article and @attychang.bsky.social for help with figures.

Segmenting cryo-electron tomography data: Extracting models from cellular landscapes Cryo-electron tomography provides an unprecedented view of cellular architecture, yet extracting meaningful biological insights remains challenging. S…

I have lots of opinions—but for your sake, I’ve limited them to segmentation in cryo-ET for this article 😉
www.sciencedirect.com/science/arti...

#teamtomo

A practical look at cryo-electron tomography image processing: Key considerations for new biological discoveries Cryo-electron tomography (cryo-ET) enables 3D visualization of complex biological environments without the need for purification, thereby preserving t…

Happy to say that my Current Opinion in Structural Biology article came out today. I hope it helps to guide to you in using cryo-ET to make new biological discoveries!

www.sciencedirect.com/science/arti...

Segmenting cryo-electron tomography data: Extracting models from cellular landscapes Cryo-electron tomography provides an unprecedented view of cellular architecture, yet extracting meaningful biological insights remains challenging. S…

At the risk of shameless self-promotion, I recently wrote a Current Opinion article on segmentation, where I briefly highlight denoising methods in the section “‘Perfecting’ imperfect data is the first step toward automated segmentation.” www.sciencedirect.com/science/arti...

Drug Repurposing Screen Identifies an HRI Activating Compound that Promotes Adaptive Mitochondrial Remodeling in MFN2-deficient Cells Pathogenic variants in the mitochondrial outer membrane GTPase MFN2 cause the peripheral neuropathy Charcot-Marie-Tooth Type 2A (CMT2A). These mutations disrupt MFN2-dependent regulation of diverse as...

New work from the lab (w/ @preronabora.bsky.social @mashiatzaman.bsky.social @mitomorph.bsky.social @nanigrotjahn.bsky.social) identifying a compound that activates OMA1-DELE1-HRI signaling to induce mitochondrial remodeling in MFN2-deficient cells. Check it out 👇
www.biorxiv.org/content/10.1...

Nobel laureate on how Trump's cuts are hurting science Neuroscientist, biologist and 2021 Nobel Prize winner Ardem Patapoutian joined Midday Edition to talk about how the Trump administration's effort to cut back on science spending is impacting researche...

I spoke with Jade Hindmon, host of @kpbssandiego.bsky.social Midday Edition, about the consequences of the current administration’s cuts to science funding. Please share to help spread the word and raise awareness.
www.kpbs.org/podcasts/kpb...

As we approach the week leading up to Juneteenth, it’s important to recognize the flag and symbols around the day, as well as taking the opportunity to renew the call for lineage-based reparations for not only chattel slavery, but the harms inflicted upon Black Americans post-emancipation.

higher res group pic 🤩

Lots of fans and supporters of @mmedina300kv.bsky.social! Grateful for the community that’s cheered her on every step of the way! @hamid13r.bsky.social @callmemich.bsky.social @attychang.bsky.social @daddea.bsky.social @nitishdua.bsky.social @reikawatanabe.bsky.social

It’s been a big week for the Grotjahn Lab—our very first graduate student, @mmedina300kv.bsky.social, successfully defended her PhD thesis! 🎉 So proud of everything she’s accomplished and grateful for the light she’s brought to our lab from day one ☀️⚡

Tying self-worth to academic success, only to ask years later, "who am I, really?"

Grateful @frezzalab.bsky.social took the time to capture this tension so beautifully in this article.

I also learned a lot from @landerlab.bsky.social about the figures-to-narrative process. I'm loving the rock and roll mechanism 🤘🎸🎶

So excited for this work to be out!! Thanks to @attychang.bsky.social, @hamid13r.bsky.social, blue sky less Daniel Fuentes, @nanigrotjahn.bsky.social and @tomo.science!!!

Thanks so much! We ❤️ #MemBrain! It is an awesome tool and a key part of our segmentation workflow. The membranes shown here are quantification-mapped surfaces from Surface Morphometrics, downstream of the MemBrain segmentations. I'm grateful for tools that integrate so well across pipelines!

thank you! Please reach out if you need help applying Surface morphometrics to your data--we're happy to help!

Grateful to keep collaborating with @tomo.science's new lab as we push the boundaries of what we can measure from cellular ultrastructure. A blue-torial breaking down how we quantify membrane thickness with Surface Morphometrics is coming soon—stay tuned! 👀