Carlo Barnaba 🇮🇹🇲🇽

I’m particularly referring to scientific hypothesis. I use often AI for trivial tasks.

I see more and more often trainees using AI to generate hypotheses. Brain is shrinking very fast nowadays.

Osprey. 🫠

Bookmark with Lincoln’s quote: To sin by silence when they should protest makes cowards of men

Found this inspirational quote as a bookmark in a used book. Very relevant.

Good catch.

With all due respect, why do you need to image at 12ms for 7 min, it is a mystery to me.

With boost to NIH budget, Senate panel rejects Trump’s plan to slash agency Republicans on spending committee push back against proposed reorganization and overhead cuts

The National Institutes of Health’s budget, and structure, would be preserved under a Senate bill for the 2026 fiscal year.

Got it. Very interesting indeed.

See, I can’t. “Dominant negative lethal” sounds so intimidating. Thanks 😊😄

Wise geneticists/CRISPRists out there. I am knocking out two genes (encoding for two isoforms) with a dual sgRNA Cas9 plasmid. I can get a dual knock-out (isoforms A and B) and a knock-out of isoform B, but no knock-out of isoform A alone. Any guess? Done this two times already, very reproducible.

Structural and cell biology NPG publication two-fer today: lysosome signaling structural biology tinyurl.com/mt3uc96k with @robzonculab.bsky.social and mitophagy initiation tinyurl.com/3rpjyfjt with @martenslab.bsky.social @lazaroulab.bsky.social and @hummerlab.bsky.social

Unnecessary suffering for wild animals and pets. 4th of July was particularly tough for my dog, who suffers from anxiety. In 2025, fireworks are just stupid.

Another reason to consider HaloTag as an alternative to GFP for tagging

Left: Lifespan of females with adult-specific, pan-neuronal induction of dMaf1RNAi(V109142) with 36R driven by Elav-GSTricoire. Right: Model of how Maf1 activity in neurons impacts aging.

#Aging reduces the ability to make new proteins, so can stimulating protein synthesis in old age be beneficial? This study shows that boosting #ProteinSynthesis in #neurons by inhibiting #RNApolymerase repressor Maf1 improves health & survival into old age in flies @plosbiology.org 🧪 plos.io/3IwLiPZ

Researchers introduce UltraID-LIPA, an innovative platform that combines the light-inducible protein aggregation (LIPA) system with the UltraID proximity-dependent biotinylation assay to identify #Parkinsons-associated α-synuclein-interacting proteins
www.cell.com/iscience/ful...

Comic

🙄

Key numbers in cell biology

Having a sense of scale helps to think more rigorously and realistically about biological systems.

Congrats Heather!!

🧑‍🔬📰🚀 #RxivMaker is here! A framework automating the creation of publication-ready scientific #preprints from simple Markdown. Goodbye manual formatting, and hello gorgeous manuscripts! A great adventure with @guijacquemet.bsky.social's lab

📜: zenodo.org/records/1575...
👨‍💻: github.com/HenriquesLab...

The expanding repertoire of ESCRT functions in cell biology and disease - Nature This Review examines recently gained insights into the roles of ESCRT complexes in viral infection, immunity, cancer and neurological disease.

The ESCRT complexes are a fascinating membrane cutting machine with roles in countless cell pathways, but they are also ubiquitous in disease and offer new opportunities as drug targets. See our new review with Alyssa Coyne, Marta Miączyńska, and Harald Stenmark at tinyurl.com/yex3trem

Scam text offering dinner with seafood pasta

Damn scam text. They know my weaknesses!

Kid with pride 🏳️‍🌈

It was important to be here, today. #pride

Another ND. Argh 😡

This is happening. A lot of scientists have lost their job already.

bsky.app/profile/barn...

65 applicants total. Many US-based researchers who have lost their job due to funding being terminated. This is bad.

I have to screen 58 applications, 100% increase from last round. A LOT of US-based postdocs whose funding have been recently lost. This is bad.

That was my question, too. It appears that NICCH, NHLBI, NIAAA, and others will be merged.

Loss of hepatic autophagy induces α‐cell proliferation through impaired glutamine‐dependent gluconeogenesis Autophagy, the highly conserved process of protein and organelle degradation, is suppressed in the liver by obesity and metabolic dysfunction-associated fatty liver disease and associated with the de...

New paper alert! Huge congrats to my graduate student Jesse Velasco on his first author publication in physiological reports. physoc.onlinelibrary.wiley.com/doi/10.14814...

This position is open until May 31st. Lots of brilliant US-based applicants this round, probably due to NIH and NSF funding issues. Unfortunately, I can only take one postdoc.