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MS Lee Lab

@msleelab.bsky.social

A research group interested in proteomics, peptidomics, and PTMomics in neuro-diseases๐Ÿ“TU_Muenchen | CLINSPECT-M consortium | opinions are my own (Chien-Yun)

39 Followers  |  41 Following  |  6 Posts  |  Joined: 06.02.2025  |  1.4048

Latest posts by msleelab.bsky.social on Bluesky

We of course couldnโ€™t let that slip and developed a method:
โœ… compatible with tandem MS
โœ… strong enrichment efficiency
โœ… uses commercial reagents, works in tubes + 96-well plates
We hope it becomes your new favorite for hunting this tiny PTM ๐Ÿ”ฅ
#citrullination #proteomics #ptms

28.04.2025 12:50 โ€” ๐Ÿ‘ 0    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
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High-throughput chemical proteomics workflow for profiling protein citrullination dynamics Citrullination is a post-translational modification implicated in autoimmune and inflammatory diseases, yet its low abundance and lack of effective enrichment tools have limited proteome-wide analysis...

Another new preprint is out โ€” with it, a proteome-wide solution to study citrullination at scale!
www.biorxiv.org/content/10.1...
If youโ€™ve been following our journey into one of the trickiest PTMs (citrullination), you might be thinking... what about enrichment before MS?

28.04.2025 12:50 โ€” ๐Ÿ‘ 0    ๐Ÿ” 0    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0

Our Motif-Specific mAbs...
โœ” Motif-specific (and sensitive)
โœ” Minimal homocitrullination cross-reactivity
โœ” Works in real samples
Also? Some commercial Abs didnโ€™t stand a chance. ๐Ÿ˜‰
To try? Weโ€™re making ours openly available to support the citrullination community!
#Citrullination #Antibodies #PTMs

31.03.2025 09:30 โ€” ๐Ÿ‘ 1    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
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Development of Motif-Specific Monoclonal Antibodies for Global Protein Citrullination Detection with Minimal Cross-Reactivity to Homocitrullination Protein citrullination, a post-translational modification (PTM) catalyzed by peptidylarginine deiminases (PADs), plays critical roles in biological processes such as immunity, gene regulation, and inf...

New preprint! ๐ŸŽ‰
www.biorxiv.org/content/10.1...
If you've ever tried detecting citrullinated proteins, you know the painโ€”most pan-citrullination antibodies are either useless, cross-reactive, or heartbreakingly bad. ๐Ÿ’”๐Ÿ˜ญ
I tested them. I suffered. I cried. So we made our own.

31.03.2025 09:30 โ€” ๐Ÿ‘ 2    ๐Ÿ” 0    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0
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Gemcitabine and ATR inhibitors synergize to kill PDAC cells by blocking DNA damage response | Molecular Systems Biology imageimagePhosphoproteomics unveils the mode of action of clinical ATR inhibitors and explains their synergy with Gemcitabine in pancreatic cancer cells. Viability screening of 146 targeted drugs ide...

๐Ÿš€ Exciting news from our lab! Our latest paper has been featured on the cover of @molsystbiol.org - "Gemcitabine and ATR inhibitors synergize to kill PDAC cells by blocking DNA damage response" by Hรถfer et al.! ๐Ÿงฌ๐ŸŽ‰ doi.org/10.1038/s44320-025-00085-6 (1/4)

04.03.2025 16:06 โ€” ๐Ÿ‘ 9    ๐Ÿ” 7    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0
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PTMNavigator: interactive visualization of differentially regulated post-translational modifications in cellular signaling pathways - Nature Communications Post-translational modifications are important regulators of cellular pathways, but our understanding of these processes is limited. Here, the authors present a web tool that integrates various databa...

๐ŸŽ‰ We're happy to announce that our latest project was published in @naturecomms.bsky.social this week: PTMNavigator, a #bioinformatics web platform for in-depth analysis of post-translational modification (PTM) perturbation datasets.
๐Ÿ“„ doi.org/10.1038/s414... (1/6)

10.01.2025 14:20 โ€” ๐Ÿ‘ 38    ๐Ÿ” 9    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 2
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oktoberfest/tutorials at development ยท wilhelm-lab/oktoberfest Rescoring and spectral library generation pipeline for proteomics. - wilhelm-lab/oktoberfest

We developed a deep learning model to predict MS2 spectra of citrullinated peptides, improving site ID by up to 14x in human tissue proteomes with high precision!
Pipeline available via oktoberfest: github.com/wilhelm-lab/...

06.02.2025 05:36 โ€” ๐Ÿ‘ 1    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
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Deep Learning Enhances Precision of Citrullination Identification in Human and Plant Tissue Proteomes Citrullination is a critical yet understudied post-translational modification (PTM) implicated in various biological processes. Exploring its role in health and disease requires a comprehensive unders...

Kicking off the new year strong! Prosit-Cit is officially online!๐Ÿป Check it out: www.mcponline.org/article/S153...

#DeepLearning #Citrullination #Bioinformatics

06.02.2025 05:36 โ€” ๐Ÿ‘ 4    ๐Ÿ” 0    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 1

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