Bas van Woudenberg

Draft genome sequence of Methylobacterium sp. OT2 isolated from human skin and capable of growing on t-octylphenol polyethoxylate | Microbiology Resource Announcements Methylobacterium strains are common on human skin, but their genomic information is limited (1, 2). Here, we report the genome sequence of a Methylobacterium strain isolated from human skin. A forehead skin swab from a female college student was streaked on minimal salts basal medium agar (3) with 0.5% Triton X-100 as a carbon and energy source. After 1 week of aerobic incubation at 28°C, one isolate, OT2, was purified on Nutrient agar (KisanBio Co) and incubated for 4 days at 28°C. The strain was deposited in the Korean Collection for Type Cultures as KCTC 82560 and Japan Collection of Microorganisms as JCM 34944 (NCBI taxonomy ID 2813779). Ethical approval for subject sampling was granted by the institutional review board of Changwon National University. Sanger sequencing of the 16S rRNA gene using primers 27F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′-GGTTACCTTGTTACGACTT-3′), along with BLASTN analysis (4), identified OT2 as a member of the genus Methylobacterium. It showed the highest similarity to M. oryzae CBMB20T (99.63%) and M. fujisawaense strain DSM 5686T (99.63%).

Your post made me think about this Methylobacterium that is able grow in triton! Happy hunting! journals.asm.org/doi/10.1128/...

Please repost!

Postdoc in adaptive laboratory evolution and C1 synthetic metabolism!

Location: DTU Biosustain

🦠🧬🔬

Starting in 01/2026!

Fell free to reach out if you have questions!
efzu.fa.em2.oraclecloud.com/hcmUI/Candid...

Methylobacterium extorquens!

Aromatic acid metabolism in Methylobacterium extorquens reveals interplay between methylotrophic and heterotrophic pathways www.biorxiv.org/content/10.1... #jcampubs

Interested in Sustainability, Metabolic Engineering and Evolution?
Check out how we convinced E. coli to grow on the CO2-derived substrate formic acid using the synthetic Serine Threonine Cycle! ♻️🌱🦠

OPEN ACCESS publication to be found here:
🚨 doi.org/10.1016/j.ym... 🚨

#MEvoSky #microsky

(1/🧵)

Beyond the pink: uncovering the secrets of pink pigmented facultative methylotrophs - Archives of Microbiology Pink Pigmented Facultative Methylotrophs (PPFMs) belong to a diverse group of methylotrophic bacteria predominantly in the genus Methylobacterium, and are known for their beneficial interactions with ...

Beyond the pink: uncovering the secrets of pink pigmented facultative methylotrophs link.springer.com/article/10.1... #jcampubs

Upcycling of polyamides through chemical hydrolysis and engineered Pseudomonas putida - Nature Microbiology Metabolic engineering guided by laboratory evolution enables upcycling of nylon by Pseudomonas putida.

www.nature.com/articles/s41...

Design and regulation of engineered bacteria for environmental release - Nature Microbiology Chemla et al. review the release of bacteria into the environment and propose engineering strategies to help improve performance and reduce risk.

REVIEW: Design and regulation of engineered bacteria for environmental release

by Chris Voigt & co

#microsky

www.nature.com/articles/s41...

Methanol transfer supports metabolic syntrophy between bacteria and archaea - Nature Zhaonella formicivorans produces methanol, for a novel syntrophic interaction, without requiring methylated compounds as an input.

Methanol transfer supports metabolic syntrophy between bacteria and archaea www.nature.com/articles/s41... #jcampubs

Designing strong inducible synthetic promoters in yeasts - Nature Communications Inducible promoters that control gene expression in response to small molecules are essential in synthetic biology. Here, the authors describe a simple and reliable approach for constructing strongly…

Eukaryotic promoters are complex, making synthetic promoter design challenging. In a recent paper, Masahiro Tominaga’s team decoded key design rules and demonstrated the potential of their inducible yeast promoters for biologic production. Learn more 👇

Led astray by 16S rRNA: phylogenomics reaffirms the monophyly of Methylobacterium and lack of support for Methylorubrum as a genus academic.oup.com/ismej/advanc... #jcampubs

Monitoring and dynamically controlling glucose uptake rate and central metabolism - Nature Chemical Engineering Glucose uptake is the initial step in cellular metabolism, and its uptake rate directly determines the overall metabolic flow. Here the authors develop a set of programmable bifunctional biosensors fo...

🚨 Out now with free access!

A set of programmable bifunctional biosensors for real-time monitoring of glucose uptake rates and dynamic dual control of glucose uptake and central metabolism. #ChemSky #ChemBio

Link: www.nature.com/articles/s44...

Measurements of translation initiation from all 64 codons in E. coli Abstract. Our understanding of translation underpins our capacity to engineer living systems. The canonical start codon (AUG) and a few near-cognates (GUG,

Some examples showing this from my group's research:
doi.org/10.1093/nar/...

doi.org/10.1101/2021...