Richard Lincoln, PhD

Full DOI: doi.org/10.1016/j.ch...

Happy to share the final chapter in the story of PaX Dyes, and my time at @stefanhelllabs.bsky.social at @mpi-mr.bsky.social! Now in @cp-chem.bsky.social, we access the red and far-red regions on the spectrum with our caging-group-free strategy for multicolor #SMLM, #STED, and #MINFLUX #microscopy!

If you’re excited about chemical biology and advanced imaging, we’d love to hear from you!

Check out the position here: www.embl.org/about/info/s...

🚀 We’re hiring! EMBL TechDev Internship (TDI25-14)

We’re looking for an organic chemist to help develop novel fluorogenic HaloTag ligands for deep-tissue imaging. You’ll contribute to molecular design and run in silico, in vitro, and microscopy-based assays to quantify fluorophore performance.

🌟Join us for our #MPIMRSpotlight series, where we shine a light on the scientists at our Institute and their exciting research! What sparks their curiosity? ✨What are they aiming to achieve? 👀Today, we're featuring @chrisheideb.bsky.social from the Optical Nanoscopy department. 🔬 Check out his work!

🔬🧠We are pleased to announce that our work on Paralemmin-1 as a powerful regulator of the neuronal submembrane cytoskeleton has been published in Science Advances @science.org! Read the full study here: www.science.org/doi/10.1126/...
#Neuroscience

📢 #Preprint Alert: For #fluorescentfriday we report minimally-sized photoactivatable PaX dyes with red and far-red emission for #STED, #SMLM, and multiplexed #MINFLUX #SuperResolution #Microscopy! A huge thanks to the Optical #Nanoscopy team at @mpi-mr.bsky.social! 🔬

www.biorxiv.org/content/10.1...

Biocompatible sulfonium-based covalent probes for endogenous tubulin fluorescence nanoscopy in live and fixed cells Fluorescent probes enable the visualization of dynamic cellular processes with high precision, particularly when coupled with super-resolution imaging techniques that surpass the diffraction limit. Traditional methods include fluorescent protein fusion (e.g., GFP) or organic fluorophores linked to ligands targeting the protein of interest. However, these approaches often introduce functional disruptions or ligand-associated biological effects. Herein, we address these challenges by developing covalent fluorescent probes for endogenous tubulin, a critical cytoskeletal protein involved in processes such as cell movement, division, and biomolecule trafficking. Using well-known tubulin binder cabazitaxel and cell permeable fluorophore silicon-rhodamine—as a basis, we introduce a novel biocompatible cleavable linker containing a sulfonium center. This allowed the construction of the optimized probe , 6-SiR- o -C9-CTX , demonstrating excellent cell permeability, fluorogenic properties, and the ability to covalently label tubulin across various human cell lines. Importantly, the targeting moiety could be washed out while preserving tubulin staining, ensuring minimal disruption of tubulin function. This labeling technique is compatible with STED nanoscopy in both live and fixed cells, offering a powerful high-resolution imaging tool. ### Competing Interest Statement G.L. is a co-inventor on the patent (EP2748173B1 and US9346957B2, applicant EPFL) describing SiR and its derivatives.

We have introduced a new biocompatible sulfonium-based building block for generation of non-cytotoxic covalent fluorescent probes. The proof-of-concept paper is on BioRxiv: https://doi.org/10.1101/2025.01.27.635008 . Great job done by @MarieAuvray4 at @mpi_nat ! Enjoy reading!

Hi there! 👋 We are the PR team of the Max Planck Institute for Medical Research in beautiful Heidelberg and very excited to join Bluesky! 😍🥳 Follow us to learn more about our research, our great people, events, awards etc! We are really looking forward to interacting with you! 🚀🗨️

Are you a cell biologist interested in nanoscale fluorescence imaging?

Check out our new review in Trends in Cell Biology:
www.sciencedirect.com/science/arti...

Thanks and kudos to all authors: Elisa DʼEste, Gražvydas Lukinavičius, Felipe Opazo and @rlicolnchemist.bsky.social