Luca Braccioli Lab @ UU

Two new preprints from the @arjunraj.bsky.social‬ on #gastruloids. Both excellent. Exploring the relationship between macroscopic reproducibility v microscopic heterogeneity. Stunning experiments and much to think about
biorxiv.org/content/earl...
biorxiv.org/content/earl...

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We have just launched the Hubrecht International PhD Program (HIPP).

Are you looking for a PhD position in molecular and developmental biology or related subject in an international, very supportive and collaborative environment?

Then apply to the HIPP!! 👇

Congratulations Adriano!!!

Excellent work supporting #modularity of the mammalian #embryo, a role for #hypoxia & how development exploits these features. It shows what we can learn from assemblies of #gastruloids @ spatio-temporal coordination of the modules in AP organization. Putting modules together, extremely informative

Fantastic work from @bulutkarslioglu.bsky.social group. we r happy to contribute on the role of hypoxia in proper gastrulation ex utero as originally noted in Aguilera-Castrejon et al. Nature 2021 for robust gastrulation. Now this is shown to be important for taking gastruloids to the next level.

Congratulations Aydan, Tasos and the rest of the team for this beautiful work!

⚠️ I am really excited to share the work of Anastasios Balaskas, an excellent PhD candidate in the lab, with the wider world. Tasos made a significant advance: generating a stem cell-based embryo model that contains both posterior and anterior neural tissues of the late-stage gastrulating embryo.

More have been found: www.bbc.com/news/article...

Congratulations Kristina, Vikas and the rest of the team!

📢 Fresh off the press and featuring new exciting experiments! 🧪
We show how glycolytic activity instructs germ layer proportions through regulation of Nodal and Wnt signaling - happy to finally share this 😊
doi.org/10.1016/j.st...

B2B with @jesseveenvliet.bsky.social lab: doi.org/10.1016/j.st... 🤩

Congratulations to the whole team!

🚨Just one week left to apply! Join our team @imbavienna.bsky.social!

Thank you! A special thanks to the organizers for putting this fantastic meeting together @moritzbauer.bsky.social @bracciolilab.bsky.social Hendrik Marks, Susana Chuva de Sousa, Lopes and Niels Geijsen. And to Wilke Meijer and Suzan Stello for working together on this exciting project!!

Well done @virginiar-andra.bsky.social from our lab for being awarded the Best Poster Prize at the International Dutch Embryo Model Meeting today for our work on “Spatiotemporally resolved omics reveals dynamic antagonistic gradients” in embryo segmentation. Congratulations!

Stem cell culture conditions affect in vitro differentiation potential and mouse gastruloid formation Aggregating low numbers of mouse embryonic stem cells (mESCs) and inducing Wnt signalling generates ‘gastruloids’, self-organising complex structures that display an anteroposterior organisation of ce...

Very happy to share that our paper on stem cell culture conditions is now out! doi.org/10.1371/jour... We found that modulating mESC culture conditions influences gastruloid elongation and lineage specification, and helps with making the protocol more reproducible in our hands! more below 🧵⬇️ [1/7]

Identifying cross-lineage dependencies of cell-type-specific regulators in mouse gastruloids Correct gene expression levels are crucial for normal development. Advances in genomics enable the inference of gene regulatory programs active during…

And to finish a hat-trick of #gastruloid papers @bracciolilab.bsky.social on the influence of epigenetics on lineage decisions shorturl.at/aA6yy

10/10

Finally, a big thanks to all the authors involved. I am very grateful to all the members of Elzo de Wit's lab @dewitlab.bsky.social and the facilities and collaborators at the Netherlands Cancer Institute @nkinl.bsky.social. What a journey it has been!

9/10

This frame work lays the foundation of my recently established lab @bracciolilab.bsky.social where we will investigate the interplay between gene regulation and inter-tissue communication!

10/10

Finally, a big thanks to all the authors involved. I am very grateful to all the members of Elzo de Wit's lab
@dewitlab.bsky.social and the facilities and collaborators at the Netherlands Cancer Institute
@nkinl.bsky.social. What a journey it has been!

8/10

In conclusion, our work provides an innovative framework for dissecting the crosstalk between gene regulation and inter-tissue communication in mammalian embryonic development.

7/10

Finally, we used chimeric gastruloids derived from a mix of wild-type (WT) and MSGN1 KO cells. This revealed that MSGN1 KO cells can form spinal cord in the presence of WT cells.
This indicates an underlying inter-tissue communication program driven by MSGN1.

6/10

Surprisingly, MSGN1 KO not only impaired mesoderm development, as anticipated, but also negatively affected spinal cord development. This reveals an inter-tissue role of MSGN1 that was previously unknown.

5/10

We were particularly struck by MSGN1 and CDX2 as potential drivers of the early decision from neuromesodermal progenitors (NMPs) to mesoderm and spinal cord, respectively.

4/10

Next, we investigated which transcription factors (TFs) are active during gastruloid development. To do this, we defined the developmental trajectories in gastruloids and predicted TF activity based on their motif accessibility.

3/10

We discovered that the chromatin regulatory profile of gastruloids strikingly matches that of post-gastrulation embryos.

2/10

We utilized single-cell ATAC-seq to analyze developing mouse gastruloids, successfully defining their chromatin regulatory profile.

1/10

Our research aimed to answer two key questions: how do pluripotent cells make early cell fate decisions? and how does gene regulation drive these decisions?

Identifying cross-lineage dependencies of cell-type-specific regulators in mouse gastruloids Braccioli et al. use time-resolved single-cell chromatin accessibility analysis to study mouse gastruloid development. They identify key transcription factors driving mesoderm and spinal cord differen...

🚨 Paper Alert 🚨
I'm incredibly proud and excited to announce that our latest research on the chromatin regulatory profile of gastruloids has been published in @DevCell! 🎉

Check out the full paper here:
www.cell.com/developmenta...

@dewitlab.bsky.social , @bracciolilab.bsky.social 🙌

🧵👇

Fantastic news!