Rik Haagmans

📣 #SPARKME update!

We're excited to announce the lineup of speakers for the #SPARKME workshop - 26-27th of May.

youngemerg.com/yemerg05.shtml

Alongside leading experts in #MECFS, we’ll feature early career researchers to present their research🌟

Lets introduce our speakers👇🧵

🔔 Deadline Extension! 🔔 The abstract submission deadline for #SPARKME has been extended until April 15th! Don’t miss your chance to contribute to this exciting workshop on May 26-27 at Wellcome Genome Campus, Cambridge, UK. 📝✨ #MECFS #MEresearch #ECR #SPARKME

🌟Exciting News! We're thrilled to announce the upcoming #SPARKME workshop, on May 26-27th, Wellcome Genome Campus, Cambridge UK 📆🔬 #SaveTheDate #MECFS #MEresearch #ECR
@investinmeresearch.bsky.social

youngemerg.com/yemerg05.shtml

GitHub - RHaagmans/mc-spike Contribute to RHaagmans/mc-spike development by creating an account on GitHub.

All the bioinformatics used in the analysis is packaged into a Nextflow pipeline available at: github.com/RHaagmans/mc...
It should be easily customisable, so who knows, it may be useful for anyone who doing any benchmarking of their method. 😊4/4

We found different recovery efficiencies between viruses, and that WTA2 gives a richer dataset with better assembly contiguity, but with higher variation compared to SISPA. On the other hand, SISPA is more economical and modular than WTA2. So there are pros and cons to each. 3/4

We compared recovery of a variety of RNA and DNA viruses, and by sequencing multiple replicates of the same sample, we compared the variation in relative abundance of viruses between these methods. 2/4

So excited that my first first-author paper is now published in Viruses! In this paper we looked at bias and reproducibility of RNA+DNA faecal virome metagenomic approaches, and compared reverse transcription and PCR amplification using WTA2 and SISPA. 1/4 www.mdpi.com/1999-4915/17...