Kent Boles

@atinygreencell.bsky.social

Now that's a hairpin!

I didn't see a patent app.

Tiny CRISPR tool opens door to faster, simpler plant genome editing Plant breeding plays a vital role in ensuring global food security by increasing crop yields, improving nutritional quality and creating crops that are adaptable to climate change. However, current pl...

stemcell.ucla.edu/news/tiny-cr...

Original paper www.pnas.org/doi/10.1073/...

Roger Tsien's work on FPs was invaluable. The original pub where they evolved mRFP1 is a great read. Here's the associated method. www.tsienlab.ucsd.edu/Publications...

You can upload a document with up to 0.5M words to notebooklm.google.com

YouTube video by FalcoVEVO Falco - Der Kommissar (U.S. Official Video) (VOD)

Did you say Falco? youtu.be/8-bgiiTxhzM?...
Too bad it's not a rocker for branding.

It's nice the lids are shared. You could develop circulation, light or sensors on the lids that could fit multiple depth / volumes.

My Walmart has a pack for $6

Plant Success Organics Soluble® Mycorrhizae - with Bacteria, Kelp and Humic Acids Plant Success Organics Soluble™ will enhance established plants with beneficial soil microbes. Description It contains mycorrhizae, bacteria and trichoderma. It is easily watered in and will instantly...

I use this one in my garden. It's quite the zoo of species. plantrevolution.com/products/sol...

It sounds backwards, but institutes could pay a subscription for access so their members could publish for free.

Living lava lamp?

Do you have the patent?

I agree, but you're talking about a 50% pay cut, at least. Also, one can expect automation to slowly take over the simple work. There's always a niche for hand-crafted, but industrialization usually takes over. Oligos and gene fragments are mostly automated...

Tolerance for variability has to be taught early on. Had a PhD physicist in a group, the bio lab meeting would frustrate him w all the bad design. I finally explained that biology is a stochastic system with millions of doors opened and shut leading to our system. He was depressed after that.

Design is cheap and scales since it's digital. Build and test are the hard parts being largely analog. We are getting better at automating, but the methods aren't universal. Hands on still competes since it's so versatile, but it doesn't scale and takes years to learn. Where to retrain and enter?

Sorry, 50bp to keep the promoter from being active on the arm.

I've done this doing serial knockouts in a haploid yeast. 500bp on one arm and only 50bp to preserve the promoter. Yeast are fairly recombinogenic, though. In eukaryotes, NHEJ competes so you ko those genes or inhibit ligases, etc.

Yes, I'm sure you'll find an activating mutation. There are quite a few in the literature since those variants lend themselves to structural studies.

Oh good, I wonder why it's so active?

Sequence it!

Author

I meant to type autoclave. Weird.
I aliquot SOC into 50ml tubes and autoclave them. The polypropylene is usually fine. My latest batch of tubes from China had some diffent plastic in the lids and they melted.

Bill Hader Eating Popcorn GIF ALT: Bill Hader Eating Popcorn GIF

I'd like to be there when you open the microwave

orcid.org/0000-0002-95...

Add me pls

I just bought an old Amersham fluorometer. I wish I knew about this one.

That's a lot of available codons

The copy control is induced to high copy during prep giving comparable yields to pACY184 etc.