Claire Dessalles

Frequency dependence of fast-lifetime FLIM and fits for C2C12 cells.

Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM by Juan Manuel Garcia-Arcos and team: www.biorxiv.org/content/10.1...

An incredibly comprehensive and meticulous study of Flipper-TR lifetime analysis to make your membrane tension measurements using Flipper-TR more robust and reliable.

Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!

All you've ever wanted to know about FLIM analysis (and its application in Flipper-TR)!😱🤩🔬

Figure 2 - Gene expression patterning and transcriptional profiles in hydrogel-grown gastruloids. (A) Representative immunofluorescence images of gastruloids at 120 h. Gastruloids were grown in standard culture conditions (Ctrl, no hydrogel) or embedded in hydrogels with increasing concentrations (0.7 mM, 0.8 mM and 1.0 mM) at 96 h post-seeding. Nuclei (blue), BRA (purple) and SOX2 (yellow) are labeled. (B,C) Normalized expression profiles of SOX2 (B) and BRA (C) along the AP axis, demonstrating consistent expression patterns across conditions. (D) Principal component analysis of bulk RNA-seq experiments (three replicates), revealing clear separation between Ctrl and noCHI (red), a negative control for gastruloid formation, while hydrogel-embedded conditions cluster near Ctrl. (E) Number of significantly differentially expressed (DE) genes compared to Ctrl, showing substantial transcriptional changes in noCHI and minimal changes in hydrogel-embedded conditions (0.7 mM, 0.8 mM and 1.0 mM). Shown here are representative data and analysis for one experimental replicate. See Fig. S3 for two additional replicates.

Modifying mechanical constraint guides gastruloid patterning

This Research Highlight showcases the work from Judith Pineau @judithpineau.bsky.social, Jerome Wong-Ng @jeromewongng.bsky.social, Thomas Gregor @thomasgregor.bsky.social and colleagues:

journals.biologists.com/dev/article/...

In this beautiful system, we could show uncoupling of gene expression profiles, morphology and patterning… #NotInTheGenes 😉 (also includes a method to do long term 🔬 of elongating gastruloids)
Thank you to all involved @jeromewongng.bsky.social @thomasgregor.bsky.social @alexandremayran.bsky.social

#FluorescenceFriday: getting ready for #GEF25, playing with Ciarán's stunning ExM data

👩‍🔬J'ai commencé un kit de démarrage "chercheuses en France" go.bsky.app/TBEzKC #FemmesEnScience

J'ai besoin de votre aide pour le remplir et donner de la visibilité aux chercheuses que vous connaissez et qui sont sur Bluesky !
▶️Taguez celles que vous connaissez, je les ajoute !

Super clear thread for super cool science!

I love the pearling instability at the end ! 😍 What happens in the happy attached flagellum to stabilize its tubular shape?

Most striking, in some instances of spontaneous flagellar detachment, the flagellum can glide on its own - mercilessly leaving the cell body behind, stabbing it on the way if need be (don't do this at home).

#SmoothMuscleCell #Piezo1 Small intestinal motility

SMC Piezo1 KO in 4~6 wk-old🐭
👉Muscularis thinning
👉⏬Food consumption
👉⏬SMC density w/o⏫apoptosis
👉⏬small intestinal motility
👉⏬Muscularis Orai1/3 Trpc4 IP3R ⏫Cav1.2 Ano1
👉⏬carbachol-induced tension

#CommsBio 2025
www.nature.com/articles/s42...

In the New and Notable "Pulling Frank-Starling into another dimension,"
Megan L. McCain highlights the paper "Biaxial length-tension relationship in single cardiac myocytes ." buff.ly/XliGXNu

Human microglia bright field image segmented with CellPose 4.

CellPose 4.0 is a beast. Segmented human microglia, in brightfield. Credit to Sydney Houser.

Congrats David, well done! 👏

Super excited that our group will be supported by an ERC Starting Grant!

In project "InfoFate" we will study how cells use information in dynamical, neighborhood & mechanical signals to make decisions.

We'll have PhD and Postdoc positions available, please get in touch if interested!

New preprint! 🚨 We uncover a slow adaptation to stretch that links star-bundling of keratin filaments with nuclear escape from its keratin cage. Led by @tomgolde.bsky.social‬ 🙌
@IBECBarcelona
www.biorxiv.org/content/10.1...

Now published in @natcomms.nature.com! 🥳

👉 rdcu.be/eATn3

We developed image analysis tools to capture the nematic orientation field of 3D tissue surfaces. Tested on epithelial aggregates, zebrafish hearts, myoblasts on spheres & micro-vessels, we combined soft matter physics with exp. biology.

Brain endothelial gap junction coupling enables rapid vasodilation propagation during neurovascular coupling Vasodilatory signals are rapidly communicated across long distances by endothelial-endothelial gap junctions, enabling coordinated dilation of the arterial network during neurovascular coupling.

Very cool new paper by @thegulab.bsky.social published in Cell, showing that arterial endothelial coupling via gap junctions determines the speed and spread of vasodilation induced by neuronal activity ! #cerebrovascular #bloodflow #endothelium

Here’s why your blood vessels don’t burst under pressure Cells lining the blood vessels reorganize their inner structures to handle stressful boosts in pressure.

Our study on endothelial mechanics made the news! 🤩

Super nice and clear article on our findings and their implications.
www.snexplores.org/article/how-...

YouTube video by Roux Lab - University of Geneva Cell forces, order and flows during In vitro reconstitution of morphogenesis

Nice discussions after the talk given online yesterday at cytophys.org, presenting the work of @pauguillamat.bsky.social @clairedessalles.bsky.social @yamini-ravichan.bsky.social @oriolmb.bsky.social and Tithi Mandal. Available on the lab youtube channel at:
youtu.be/g-7HomnPdtk

RCF winter call results: funding decisions in biosciences, health and environmental research The Research Council of Finland’s Scientific Council for Biosciences, Health and the Environment has granted funding for 38 Academy Research Fellowships, 12 Clinical Researchers and 76 Academy P...

I'm grateful for the Research Council of Finland for granting me the Academy Research fellowship! After getting over the shock I will soon move back to Finland to start as a group leader at the Institute of Biotechnology @helsinki.fi. Looking for a PhD student soon! www.aka.fi/en/about-us/...

Blood vessels under stress Writing in Nature Physics, Claire Dessalles, Avin Babataheri, Guillaume Salbreux, and colleagues show the effect of luminal pressure on cytoskeletal dynamics and tissue mechanics in an experimental mo...

Thank you @cp-newton.bsky.social for the very nice spotlight on our latest paper on endothelial mechanics!
www.cell.com/newton/fullt...

Hi everyone! We are Newton, the flagship physics journal for Cell Press that launches in 2025. Starting our first post with an

🎇 EXCITING ANNOUNCEMENT 🎇

Our first article and Perspective are now online!!! Check them out @ www.cell.com/newton/newar... !

How can organs regenerate fast? Bioelectricity helps -- by coupling sub-second electrical injury signals to regenerative proliferation.

First preprint from my potsdoc :D with @liujinghui.bsky.social @ritamateus.bsky.social @mpi-cbg.de and collaborators @mpipks.bsky.social @csbdresden.bsky.social

Super proud of superstar postdocs @nerlielisa.bsky.social and @liujinghui.bsky.social for our lab's most recent preprint! We uncover how wound-induced electrical currents are generated and transmitted in tissues, triggering proliferation, essential for organ #regeneration #bioelectricity #QBio 👇

Re-growing adult organs: mothers do it best - the Node Tomotsune Ameku tells the story behind the paper "Growth of the maternal intestine during reproduction"

Re-growing adult organs: mothers do it best

Tomotsune Ameku from the lab of @irenemiguel-aliaga.bsky.social @crick.ac.uk tells the behind the paper story of their work looking into maternal intestinal growth in mice during reproduction:
thenode.biologists.com/re-growing-a...

Advection versus diffusion in brain ventricular transport Cerebrospinal fluid (CSF) is integral to brain function. CSF provides mechanical support for the brain and helps distribute nutrients, neurotransmitters and metabolites throughout the central nervous ...

Preprint alert: 🧠🌊🦓-🐟
Have you ever wondered how molecules move around the brain?
Check out our latest preprint led by Halvor Herlyng from the team of Marie Rognes @utropstegn.bsky.social at @simularesearch.bsky.social and us at NTNU.
www.biorxiv.org/content/10.1...

Nematics in action in vivo! Smooth muscle cells (mis-)orientation and their active stress matters to dorsal aorta function.

Happy to officially introduce FilaBuster - a strategy for rapid, light-mediated intermediate filament disassembly. Compatible with multiple IF types, modular in design, and precise enough to induce localized filament disassembly in live cells.

www.biorxiv.org/content/10.1...

Flocking and active solid behavior of epithelial cells in our new study in @pnas.org
Great work of Y. Shen, J. O'Byrne and
@andreasschoenit.bsky.social and collaboration with A. Maitra and R. Voituriez!
| PNAS www.pnas.org/doi/10.1073/...