Andrey Aristov

Culture of pluripotent stem cells in microscale droplets modulates differentiation and tissue patterning towards organoids on chip - Stem Cell Research & Therapy Background The differentiation of pluripotent stem cells (PSCs) and their self-organization into organoids are influenced by cell-cell interactions mediated by contacts and secreted molecules. These interactions are enhanced in microfluidic droplets due to confinement and small culture volumes. However, a comprehensive study on the culture of PSCs within droplets and the impact of this microenvironment has yet to be conducted. Methods In this study, we present a droplet platform for the 3D culture of PSCs at various stages of cellular commitment, such as pluripotency, spontaneous differentiation and directed differentiation towards gastruloids and cardioids. Results We demonstrate PSC differentiation into the three germ layers and the feasibility of organoid formation within droplets. Our findings reveal that culturing PSCs in confined volumes regulates cell fate decisions, promoting tissue patterning in gastruloids through the sequential induction of growth and migration of distinct differentiated cell populations, and facilitating the self-organization of cardiac organoids. Conclusion This technological approach provides unique insights into the intrinsic factors regulating tissue self-patterning in vitro, with potential applications in drug screening and disease modeling.

The full open-access paper is here: stemcellres.biomedcentral.com/articles/10....

Sometimes, making things smaller lets cells show us something bigger.

We explored how tiny droplets — just a few microliters in size — can change the way pluripotent stem cells grow, decide their fates, and even allow them to self-organize into mini-tissues like cardiac organoids (yes, as you can see, they actually beat 💓).

I'm very glad to share that the main outcome of my postdoc at Institut Pasteur has just been published in Stem Cell Research & Therapy! 🔬 We explored how tiny droplets — just a few microlite... I'm very glad to share that the main outcome of my postdoc at Institut Pasteur has just been published in Stem Cell Research & Therapy! 🔬 We explored how tiny droplets — just a few microliters in si...

New paper alert! My former team from Baroud Lab @pasteur.fr just published a paper in Stem Cell Research & Therapy! 🔬

Continuous mode Shazam ftw!

My smaller daughter consumes mango with the skin as well.

Great to have Andrey visit and show me what he's up to these days for big image data analysis!

Friday visit of @christlet.bsky.social lab in Marseille. I could finally see where his marvelous images come from!

The region in Russia opposing Alaska is called Chukotka.