Maurice Lee

Imaging of cellular dynamics from a whole organism to subcellular scale with self-driving, multiscale microscopy Nature Methods - A self-driving multiresolution light-sheet microscope enables the simultaneous observation and quantification of cellular and subcellular dynamics in the context of intact and...

The self-driving multiscale microscope from @daetwylerstephan.bsky.social is out.

It can image an entire zebrafish embryo longitudinally and follow a volume of interest over time with high resolution.

We used it to study cancer-immune cell interactions.

www.nature.com/articles/s41...

Goodbye Mr Snouty. I had a lot of fun working with you. It has been a blast. Hopefully the next scientist/engineer who works with you will enjoy the process too.🥹

Science bio-imaging question:

What platform are people liking for confocal / super-resolution imaging these days? Leica? Zeiss? Olympus? Nikon? Other?

I like our Andor Dragonfly spinning disk confocal with two Sona sCMOS cameras. It's connected to a Nikon Eclipse Ti2 body. The powerful lasers, high NA Nikon objectives, and Nikon's PFS autofocus system allow us to easily do SMLM.

You can always Facebook message me, Dave!😆 I hope you and the family are doing well!

Hello science friends on bluesky!
After many years of doing molecular biophysics and optics/microscopy, I just want to share that I'm leaving science and academia next month. I don't have concrete plans for my next step yet so I might just take a break for a while but we shall see how things go.

Also in today's preprint trawling: imaging at depth is difficult, so let's just... squish cells? arxiv.org/abs/2411.12656

Everything is 2D when you squish them enough!

Probe set selection for targeted spatial transcriptomics - Nature Methods Spapros is a probe set selection pipeline for targeted spatial transcriptomics that optimizes for both transcriptional and within-cell type variation.

search.app?link=https%3...

I think what I learned from staring at your picture is that in an infinitely large optical table with an infinite number of configurations, all the microscopes ever built by our finite minds exist, including those that have bad aberrations and those that have perma oil stains on them

Does anyone know any good publications/databases to find non-coding variant loci for neurodevelopmental disorders? (anything, but particularly disorders of brain size if possible)

Efficient and highly amplified imaging of nucleic acid targets in cellular and histopathological samples with pSABER - Nature Methods pSABER combines the power of signal amplification by exchange reaction (SABER) with the deposition of fluorescent or colorimetric substrates by horseradish peroxidase to enable enhanced signals for in...

The pSABER paper is now online!

pSABER is a new method for amplifying in situ hybridization signals

This work was driven by Sahar Attar and co-led by Ram Akliesh ([at] podocytes on the other app) with help from @shechnerlab.bsky.social @dschweppe.bsky.social

doi.org/10.1038/s415...

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Caring for your friends in science means lending them your neutral density filter wheel to see if it will help to alleviate their microscope problems

please don't do this.
but i do have objectives that are broken and beyond repair. and i also do have immersion oil in the lab. and i also do have an optical table. hmmm

Calling all super-resolution microscopy enthusiasts!

Join us in Leeds for an inspiring one-day event and hear the latest developments in #SuperResolutionMicroscopy from amazing invited speakers.

Don’t miss your chance to connect, learn, and share insights! Register & submit abstracts now!

Hello everyone! So many of you have followed me over the past several days. I'm guessing most are interested in my science research work (i.e., fluorescence microscopy, image analysis, spatial transcriptomics). So I decided to wind down my bird app account and slowly post a little more on Bluesky.

Made a starter pack for microscopy and image analysis - please (self-)nominate additions!

go.bsky.app/Nwa91pC

I had the opportunity to conduct a hands-on workshop on basic optical alignment today!
Zesheng and Alex, from Dr Liang Kaicheng's group, joined me in the optics lab where we covered the basics such as "walking" the laser beam, collimating lasers, and checking back reflections!

21st Annual Advanced Imaging Methods Workshop The Advanced Imaging Methods Workshop is a 3-day event focused on new and emerging optical microscopy techniques and their applications.

The 21st Annual Advanced Imaging Methods Workshop is a 3-day event focused on new and emerging optical microscopy techniques and their applications hosted by the CRL Molecular Imaging Center at UC Berkeley on 23-25 Jan 2024.
www.eventbrite.com/e/21st-annua...

I think these are my favorite forever stamps ever!🖤🤍♥️💚🩵🧡

Google and the Department of Defense are building an AI-powered microscope to help doctors spot canc... The AI-powered tool is called an Augmented Reality Microscope, or ARM, and Google and the Department of Defense have been quietly working on it for years.

AI powered microscope to spot cancer? Built by Google and DOD?
www.cnbc.com/2023/09/18/g...

The longevity secrets of Singapore, the 6th blue zone city where people are living the longest, happ... The five health and happiness secrets of Singapore, "blue zone 2.0—the next frontier of aging."

Singapore's a blue zone city!?!?! I guess my life expectancy is ~81
finance.yahoo.com/news/longevi...

We have started to work on 3D spatial transcriptomics of the whole larval zebrafish brain and I'm excited to share an image that we recently acquired in the lab!
Every bright spot or blob represents the RNA molecules that we've labelled and imaged!
The image below is 1750*1050 microns.

The quest to map the mouse brain By combining single-cell sequencing with methods to map the spatial location of gene expression, scientists are unravelling the extraordinary cellular diversity of the brain. By combining single-cell ...

"By combining single-cell sequencing with methods to map the spatial location of gene expression, scientists are unravelling the extraordinary cellular diversity of the brain."
https://www.nature.com/articles/d41586-023-02559-9

Dr Waters is searching for an advanced microscopy fellow!
https://microfellows.hms.harvard.edu/apply/

Yes! I feel like I'm working on a technology of the future now.
"we foresee that spatial omics will likely evolve toward three-dimensional spatial omics (operating on whole organs or even organisms)"
https://www.science.org/doi/10.1126/science.abq4964

This is a photo of the night sky. The contrast has been heavily adjusted such that the stars can still be visible as white spots in a black night sky despite the light pollution from the city below. We can see a few cranes at a construction site at the bottom of the photo

I'm still amazed that my Pixel 7 can see stars with the astrophotography mode amidst all that light pollution in Singapore.

This is a photo of urban landscape with buildings and a skybridge connecting some of the buildings. There is a cloudy sky with patches of blue in the background. The sun is very bright at the top left of the photo while it is behind the white clouds.

It was a sunny day at #BiopolisSingapore

This is a figure with two different coloured histograms for the thicknesses of the coverslips from two brands which are Werner and Bioptechs. The Bioptechs coverslips are slightly thicker than the Werner coverslips on average.

And the results are in!
The #1.5 glass coverslips that we have are all thicker than the 170 microns I expected. The range is supposed to be 150-190 microns. And different brands of coverslips have slightly different distributions too!