Bhavesh Parmar's Avatar

Bhavesh Parmar

@thatmassspecguy.bsky.social

High-throughput proteomics and low-throughput SM-banter.

169 Followers  |  196 Following  |  21 Posts  |  Joined: 11.11.2024  |  1.7816

Latest posts by thatmassspecguy.bsky.social on Bluesky

The location and food will make it impossible for anyone else to ever make a better EUPA conference. Thank you for organising such an excellent event! #EUPA2025

19.06.2025 11:24 โ€” ๐Ÿ‘ 8    ๐Ÿ” 1    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0

Thank you all the colleagues that reached out in person and via DMs to share their thoughts, organisers for giving me the opportunity and amazing chair Martha Ingola and @mtrost.bsky.social

18.06.2025 11:47 โ€” ๐Ÿ‘ 1    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
Post image

How often do you get to talk at a conference with views like this? Had an amazing time sharing my research at #eupa2025.

18.06.2025 11:45 โ€” ๐Ÿ‘ 0    ๐Ÿ” 0    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0
Post image

Gearing up for #EuPA2025 next week & super excited to share my work on combining siRNA screen with translation/degradation proteomics. I mean, look at that auditorium!! ๐Ÿ˜ฎ Want to know how you can do high-throughput proteomics without a liquid handling robot or a high speed TOF like its 2020? join me

10.06.2025 12:52 โ€” ๐Ÿ‘ 1    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
Preview
#fcqc2025 | Bhavesh Parmar Last week at #FCQC2025 was an incredible experience with a stellar lineup of leading scientists in the field. Icing on the cake was listening to vishva dixitโ€ฆ

www.linkedin.com/posts/thatma...

03.04.2025 15:54 โ€” ๐Ÿ‘ 2    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

Very excited for Fast & Fair peer review - authors get decisions, with reviews, within 7 business days of submission. Reviews are high quality - lots of quality control by our academic editors, plus we pay reviewers.

25.03.2025 11:25 โ€” ๐Ÿ‘ 29    ๐Ÿ” 9    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 1

๐Ÿคทโ€โ™‚๏ธ the company rep says you can reuse them and weโ€™ve had a good experience working with 100ng-35ug. But perhaps making your own tips might be cheaper.

24.03.2025 14:16 โ€” ๐Ÿ‘ 1    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

Affinisep

24.03.2025 07:34 โ€” ๐Ÿ‘ 1    ๐Ÿ” 0    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0

During Masters thesis under Prof. Paul Haynes and Prof. Mark Molloy (Macquarie University) circa 2012-14, and a lot more as a technician at a core facility 2014-17 with @itsbinir.bsky.social hanging around.

20.03.2025 13:35 โ€” ๐Ÿ‘ 3    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

Not sure, but we usually get >90% 0 missed cleavage with STrap and SP3 at 47C, 60 mins in 50mM TEAB/EPPS.

18.03.2025 18:51 โ€” ๐Ÿ‘ 2    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
Preview
Addressing NHS chemistry: Efficient quenching of excess TMT reagent and reversing TMT-over labelling in proteomic samples by methylamine N-Hydroxysuccinimide (NHS) ester chemistry is used extensively across proteomics sample preparation. One of its increasingly prevalent applications is in isobaric reagent-based quantitation such as iT...

A basic solution to a very common problem.

collab with @kusterlab.bsky.social and @bendavisgroup.bsky.social

www.mcponline.org/article/S153...

13.03.2025 14:24 โ€” ๐Ÿ‘ 19    ๐Ÿ” 7    ๐Ÿ’ฌ 2    ๐Ÿ“Œ 1

The analogy of omics data normalization and cooking:
some processing is usually necessary, overcooking makes it bland, highly over-processed foods are unhealthy, and the quality of the ingredients matters.

27.02.2025 08:01 โ€” ๐Ÿ‘ 76    ๐Ÿ” 23    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 5

What is up with @novonordisk.bsky.social ? ๐Ÿ“‰

15.02.2025 15:18 โ€” ๐Ÿ‘ 0    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

Congrats Tina!

10.02.2025 19:24 โ€” ๐Ÿ‘ 0    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

Trying to find who is, and how. But Iโ€™m afraid many tribrids and QEs will need to be retired and replaced with TOFs if labs/core facilities are to keep up.

08.01.2025 00:10 โ€” ๐Ÿ‘ 0    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

Any specific reason for OT-OT instead of OT-IT?

07.01.2025 17:34 โ€” ๐Ÿ‘ 0    ๐Ÿ” 0    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0

Havenโ€™t tried DIA on lumos but Iโ€™m inclined to believe 2hrs/sample with FAIMS-OT-IT might actually be faster for the same quant ids.

07.01.2025 14:33 โ€” ๐Ÿ‘ 0    ๐Ÿ” 0    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0

Similar for us on ascend but for lumos which doesnโ€™t support RTS, SPS-MS3 with 24 TMT18 fractions ends up being 2 days for about 8k+ quant. With large batches and IRS channel, the throughput feels very 2018(?).

07.01.2025 14:33 โ€” ๐Ÿ‘ 1    ๐Ÿ” 0    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0

โ€˜30spdโ€™ == anyone getting TMT16 or TMT32 fractions finished within a day with >8k proteins?

06.01.2025 17:54 โ€” ๐Ÿ‘ 0    ๐Ÿ” 0    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0

Hey #TeamMassSpec, with all the super duper ultra fast TOF based DIA methods out there, how are you managing the throughput & depth on your old tribrids, QEs and Exploris? Any TMT aficionados out here getting 30spd, for instance, or even 15 with >8k proteins?

06.01.2025 17:50 โ€” ๐Ÿ‘ 3    ๐Ÿ” 4    ๐Ÿ’ฌ 3    ๐Ÿ“Œ 0

Happy holidays, and a reminder to ALWAYS check your synthesised peptide standards for isotope purity. #teammassspec

24.12.2024 21:48 โ€” ๐Ÿ‘ 2    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

#TeamMassSpec represent at the first symposium on advances in therapeutic approaches.

09.12.2024 07:16 โ€” ๐Ÿ‘ 0    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
Post image

Forgot to introduce myself on here so here you go. Hello #teammassspec, Iโ€™m a 3rd year postdoc with 13 years of experience doing proteomics at all career stages (MSc, technical scientist, JRF, PhD). India>Australia>Belgium>Germany. Hereโ€™s me a couple of weeks ago at #HUPO Dresden

17.11.2024 16:16 โ€” ๐Ÿ‘ 4    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

Hi Alejandro, would love to be added too. Cheers!

17.11.2024 16:02 โ€” ๐Ÿ‘ 1    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

actively muting/hiding posts & people in an attempt to curate my algorithm better this time around. #godspeed

14.11.2024 13:05 โ€” ๐Ÿ‘ 2    ๐Ÿ” 0    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0

@thatmassspecguy is following 20 prominent accounts