INOUE lab. (ISSP, Univ. Tokyo)

Mitochondrial origins of the pressure to sleep - Nature Research on Drosophila neurons shows links between the need to sleep and aerobic metabolism, indicating that the pressure to sleep may have a mitochondrial origin.

Mitochondrial optogenetics using delta-rhodopsin the discovery of a new elements of the origin of sleep pressure😳
www.nature.com/articles/s41...

we got the cover!! www.biophys.jp/biophysics_a...

Two papers from our lab were featured as cover art—Takashi’s bestrhodopsin mimicking GR for the Supplementary Cover of JPC Lett., and Takahashi-kun’s HulaChrimson for the Cover Picture of BPPB! ✨🥳
pubs.acs.org/toc/jpclcd/1...
www.biophys.jp/biophysics_a...

Origin of the Red-Shifted Absorption Maximum in Channelrhodopsin Chrimson pubs.rsc.org/en/content/a...

v2 in bioRxiv!! Oceanic cyanobacterial photosynthesis is negatively affected by viral NblA proteins www.biorxiv.org/content/10.1...

🧪🦠New study by Bluetools partners at Technion and collaborators shows how marine archaea use HeimdallRs to harvest a broader range of sunlight. Published in Nature Microbiology:  https://www.bluetools-project.eu/how-marine-archaea-use-light-a-closer-look-at-heimdallrs/
#MarineMicrobes #Rhodopsins

Multistep 11-cis to All-trans Retinal Photoisomerization in Bestrhodopsin, an Unusual Microbial Rhodopsin Rhodopsins constitute a broad class of retinal-binding photoreceptors. Microbial rhodopsins are canonically activated through an all-trans to 13-cis photoisomerization, whereas animal rhodopsins are m...

Multistep 11-cis to All-trans Retinal Photoisomerization in Bestrhodopsin, an Unusual Microbial Rhodopsin pubs.acs.org/doi/full/10....

Survival strategies of planktonic organisms in alpine lakes and beyond www.tandfonline.com/doi/full/10....

CryoRhodopsins: A comprehensive characterization of a group of microbial rhodopsins from cold environments Spectroscopy, structural, and functional study of microbial rhodopsins with characteristic long-living near–UV-absorbing state.

CryoRhodopsins: A comprehensive characterization of a group of microbial rhodopsins from cold environments www.science.org/doi/10.1126/...

Congratulations to Hiroto on his first “first-author” paper. HulaChrimson was found through metatranscriptome analysis from Lake Hula by the Oded group!🎉🥳 Thank you for the fantastic collaboration!🙌
@bejalab.bsky.social @shaifain.bsky.social‬

A new paper led by Hiroto (M2 student) on the novel channelrhodopsin HulaChrimson is now out in BPPB!✨ Interestingly, despite high similarity to red-absorbing Chrimson and sharing key residues, HulaChrimson shows blue absorption🫐🧐 www.jstage.jst.go.jp/article/biop...
#OpenAccess

Selective Choice of the Efficient Carotenoid Antenna by a Xanthorhodopsin: Controlling Factors for Binding and Excitation Energy Transfer Despite extensive research on carotenoids and microbial rhodopsins in aquatic environments, a fundamental understanding of the binding requirements of carotenoids that serve as auxiliary light-harvest...

Selective Choice of the Efficient Carotenoid Antenna by a Xanthorhodopsin: Controlling Factors for Binding and Excitation Energy Transfer pubs.acs.org/doi/10.1021/...

"Structural and functional characterization of human sweet taste receptor"😳
nature.com/articles/s41...
@nature.com

RubyACRs Enable Red-Shifted Optogenetic Inhibition in Freely Behaving Drosophila Optogenetic activators with red-shifted excitation spectra, such as Chrimson, have significantly advanced Drosophila neuroscience. However, until recently, available optogenetic inhibitors required sh...

Optogenetic inhibition of Drosohila neurons using RubyACRs 🟥✨
www.biorxiv.org/content/10.1...
@biorxivpreprint.bsky.social

A Constructive Study Based on Gloeobacter Rhodopsin to Explore the Origin of Extreme Redshift and Nontypical Isomerization of Bestrhodopsin pubs.acs.org/doi/10.1021/...

Our new study by Takashi, Yuma, and Masae on color-tuning mechanism of bestrhodopsin was published in JPC Lett.!🙌✨By introducing its residues into GR, we revealed the role of the counterion triad and key residues for red-shifted absorption and unique isomerization😊
pubs.acs.org/doi/10.1021/...

Time-resolved Raman study on GtACR1 has been published in JPC Lett.!🥳 Led by Dr. Shibata and in collaboration with Prof. Akiyama's and Prof. Kato(@emekato.bsky.social)'s teams, we explored structural and H-bond changes of retinal after its photoisomerization.📊✨
pubs.acs.org/doi/10.1021/...

Our collaborative study with Prof. Béjà's and Prof. Nuruki's groups has been released as a preprint on bioRxiv.
We studied carotenoid-binding rhodopsin from Antarctic cyanobacteria🦠🐧🧊

Marine Asgard Archaea's Light-Harvesting Rhodopsin Revealed In a groundbreaking advancement that challenges existing paradigms of microbial photobiology, researchers have unveiled novel structural insights into how marine Asgard archaea harness light energy.

Marine Asgard Archaea’s Light-Harvesting Rhodopsin Revealed scienmag.com/marine-asgar...

Super-resolution imaging of native fluorescent photoreceptors in chytrid fungal eyes | The EMBO Journal imageimageFungal zoospores have heterodimeric rhodopsin-guanylyl cyclase complexes for light perception, including highly fluorescent neorhodopsin (NeoR). Native NeoR can be visualized in living cells...

Super-resolution imaging of NeoR fluorescence in the chytrid fungal eyes🦠 Congratulations for Matthias and his team!👏
www.embopress.org/doi/full/10....
@embojournal.org #OpennAccess

HeimdallRs were found in new planktonic Heimdall archaea⛵️🦠 These proteins use carotenoids to capture blue light efficiently in the deep sea. At Inoue lab, Marin & Masae studied their light-harvesting efficiency and the effect of carotenoid binding on rhodopsin structure and properties. (2/2)

Our new paper on HeimdallR, carotenoid-binding rhodopsins from Heimdall archaea—one of the closest relatives of eukaryotic ancestors—has been published in Nat. Microbiol.!📄✨ This is an international collaboration work!🌏 (1/2)
doi.org/10.1038/s415...
@natmicrobiol.nature.com #OpenAccess

Very happy to see our work published in Chemical Science! No new data compared to preprint, but the text is expanded for clarity and to cite all the relevant literature. Looking forward to engineering more rhodopsins and membrane proteins!

pubs.rsc.org/en/content/a...

A key spectral tuning site of UV-sensitive vertebrate non-visual opsin Opn5 Opsins are photoreceptive proteins responsible for visual and non-visual photoreceptions in animals. In general, vertebrates have multiple visual and non-visual opsins whose spectral sensitivities ran...

Opn5の紫外吸収の鍵となるアミノ酸に関する、京都大・七田先生、山下先生らによるご研究😳
Research by Dr. Shichida, Dr. Yamashita, and their colleagues at Kyoto University on the key amino acid in the ultraviolet absorption of Opn5😳
biorxiv.org/content/earl...
@biorxivpreprint

An FTIR study on the deprotonation reaction of the retinal chromophore during the Lumi state formation in blue cone visual pigment by Mizuno-san @ Kandori-sensei's lab. Congratulations!✨🎉
sciencedirect.com/science/arti...
@biophysj.bsky.social

The structure of human sweetness In humans, the detection and ultimately the perception of sweetness begin in the oral cavity, where taste receptor cells (TRCs) dedicated to sweet-sen…

The cryo-EM structure of the human sweet receptor in Cell😳
www.sciencedirect.com/science/arti...
@cellcellpress.bsky.social

Review article on "Emerging Patterns in Membrane Protein Folding Pathways" including bacteriorhodopsin.
www.annualreviews.org/content/jour...
@annualreviews.bsky.social #OpenAccess

WOW!!! Structural basis for no retinal binding in flotillin-associated rhodopsins www.biorxiv.org/content/10.1...

５月は柏キャンパスが一番気持ち良くなる季節です☀️🌲🌳😊

May is the most pleasant season on the Kashiwa Campus. ☀️🌲🌳😊

Retinal to Retinal Energy Transfer in a Bistable Microbial Rhodopsin Dimer Neorhodopsin (NeoR) is a newly discovered fungal bistable rhodopsin that reversibly photoswitches between UV- and near-IR absorbing states denoted NeoR367 and NeoR690, respectively. NeoR367 represents...

Retinal to Retinal Energy Transfer in a Bistable Microbial Rhodopsin Dimer pubs.acs.org/doi/10.1021/...