Ryota IINO 飯野亮太

DNA Motors Powered by Exonuclease III for Autonomous Rolling Motion and Biosensing Applications Nucleic acid-based synthetic motors emulate key behaviors of biological machines, enabling applications in biosensing and nanoscale actuation. Among the reported synthetic motors, RNase H-powered moto...

DNA Motors Powered by Exonuclease III for Autonomous Rolling Motion and Biosensing Applications
www.biorxiv.org/content/10.1...

Hot Charge Carriers Mediate Regioselective Thiol Cleavage and Biofunctionalization of Gold Nanoparticles Laser excitation of plasmonic particles represents an attractive avenue to tailor and catalyze surface chemical reactions at the nanoscale. However, it remains unclear how interfacial chemistry is cat...

Hot Charge Carriers Mediate Regioselective Thiol Cleavage and Biofunctionalization of Gold Nanoparticles | ACS Applied Materials & Interfaces pubs.acs.org/doi/full/10....

Next week I will attend the 2025 East Asian Single-Molecule Biophysics Symposium and give an invited talk. Thank you very much for the wonderful opportunity! I look forward to reuniting with old friends and meeting new ones!
www.physics.nus.edu.sg/easmb2025/

Fluorescent Thermometers Based on Carbon Quantum Dots with Various Detection Modes for Intracellular Temperature Measurement We report that carbon quantum dots (CQDs) synthesized via a hydrothermal process using anthraquinone derivatives and l-cysteine provide versatile detection modes, making them suitable for various expe...

Fluorescent Thermometers Based on Carbon Quantum Dots with Various Detection Modes for Intracellular Temperature Measurement | Nano Letters pubs.acs.org/doi/full/10....

Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature Imaging with a Genetically Encoded Nanothermometer Despite improved sensitivity of nanothermometers, direct observation of heat transport inside single cells has remained challenging for the lack of high-speed temperature imaging techniques. Here, we ...

Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature Imaging with a Genetically Encoded Nanothermometer | Nano Letters pubs.acs.org/doi/10.1021/...

Active fluctuations of cytoplasmic actomyosin networks facilitate dynein-driven transport Inside cells, molecular motors transport cargo through a highly crowded cytoplasmic environment. While such an environment is assumed to hinder transport, its precise effect remains unclear. Here, we ...

Active fluctuations of cytoplasmic actomyosin networks facilitate dynein-driven transport: iScience www.cell.com/iscience/ful...

Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases Although metagenomic sequencing has revealed a rich microbial biodiversity in the mammalian gut, methods to genetically alter specific species in the microbiome are highly limited. Here, we introduce ...

Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases | Science www.science.org/doi/10.1126/...

Virion motility of sialoglycan-cleaving respiratory viruses - npj Viruses npj Viruses - Virion motility of sialoglycan-cleaving respiratory viruses

Virion motility of sialoglycan-cleaving respiratory viruses
www.nature.com/articles/s44...

Receptor Density-Dependent Motility of Influenza Virus Particles on Surface Gradients Influenza viruses can move across the surface of host cells while interacting with their glycocalyx. This motility may assist in finding or forming locations for cell entry and thereby promote cellula...

Receptor Density-Dependent Motility of Influenza Virus Particles on Surface Gradients | ACS Applied Materials & Interfaces pubs.acs.org/doi/full/10....

Introduction: Soft Robotics

Introduction: Soft Robotics | Chemical Reviews pubs.acs.org/doi/full/10....

International Conference on Biophysics and Biomedical Sciences: The 64th Annual Meeting of the Biophysical Society of Japan and the Federation Meeting of Korean Societies for Biomolecular and Bioph... Chair of ICBBS2026Sam-Yong Park (Yokohama City University)We are pleased to invite symposium proposals for International Conference on Biophysics and Biomedical Sciences (ICBBS2026).The Biophysical So...

Call for Symposium Proposals.
International Conference on Biophysics and Biomedical Sciences (ICBBS2026): The 64th Annual Meeting of the Biophysical Society of Japan and the Federation Meeting of Korean Societies for Biomolecular and Biophysical Sciences
www.biophys.jp/news/lib/new...

Fuel-Free Rolosense: Viral Sensing Using Diffusional Particle Tracking High-sensitivity viral diagnostics typically use PCR to detect and amplify viral nucleic acids which requires fluorescence reporters, enzymatic amplification, specialized equipment and can be time-c...

Fuel-Free Rolosense: Viral Sensing Using Diffusional Particle Tracking | ACS Sensors pubs.acs.org/doi/full/10....

YouTube video by The Biophysical Society of Japan Chika Okimura publishes an article in Biophysics and Physicobiology on 28 October, 2025.

Review Article (Invited)
Stress fiber contraction induces cell body rotation in single keratocytes.
Chika Okimura
Biophysics and Physicobiology, 2025, 22, e220023
www.youtube.com/watch?v=6TSr...

Plastics recycling is in trouble
Consumer product companies have walked back their plastics sustainability goals, and many recyclers are closing their doors
cen.acs.org/environment/...

Functional characterization of red-shifted rhodopsin channels from giant viruses explored by a machine-learning model for long-wavelength optogenetics www.biorxiv.org/content/10.1...

The Demon Hidden Behind Life's Ultra-Energy-Efficient Information Processing -- Demonstrated by Biological Molecular Motors The remarkable progress of artificial intelligence (AI) has revealed the enormous energy demands of modern digital architectures, raising deep concerns about sustainability. In stark contrast, the hum...

The Demon Hidden Behind Life's Ultra-Energy-Efficient Information Processing -- Demonstrated by Biological Molecular Motors
Toshio Yanagida, Keisuke Fujita, Mitsuhiro Iwaki
arxiv.org/abs/2510.27212

interestingly, we predicted this based on solely macroscopic principles:

"Based on the diameters of the input gear and output gear, and assuming an equal density of gear teeth, we can estimate a ttheoretical mechanical advantage (and so [...] a torque multiplication) of ∼6."
doi.org/10.1016/j.ch...

Torque-generating units of the bacterial flagellar motor are rotary motors E. coli swims using helical flagellar filaments driven at their base by a rotary motor. Torque-generating ‘stator’ units drive the bacterial flagellar motor (BFM) by transmitting mechanical power to a...

Torque-generating units of the bacterial flagellar motor are rotary motors
"The MotB dimer rotates at the same angular speed as the cell body"
"The MotA pentamer rotates ∼6.2x faster than the flagellar motor"
細菌べん毛固定子の回転計測。蛍光偏光褪色を利用。
www.biorxiv.org/content/10.1...

Single-molecule friends!

The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:

Have a look, tell your friends, and register!

The Next Dimension: Digital Holography for 3D Interferometric Scattering We provide detailed experimental guidelines for implementing digital holography in the context of high-sensitivity interferometric scattering (iSCAT)-based nanosizing applications. Our approach relies on interferometry via the highly versatile off-axis implementation of digital holography, which offers key advantages over more traditional strategies. After a brief theoretical discussion of off-axis holography and its differences and similarities with iSCAT, typical experimental implementations and digital data-processing steps are presented. Key experimental parameters and strategies to achieve optimal performance are also highlighted. Following these experimental aspects, we focus on digital postprocessing routines that enable digital refocusing and 3D particle tracking as well as pupil function aberration correction. We then conclude with a few examples highlighting the broad applicability of digital holography for nanosizing and particle characterization applications, as well as an outlook for future applications.

The Next Dimension: Digital Holography for 3D Interferometric Scattering | ACS Nano pubs.acs.org/doi/10.1021/...

Structures of rotary ATP synthase from Thermus thermophilus during proton powered ATP synthesis Cryo-EM snapshots of the ATP synthase driven by proton motive force reveal the torsional deformation during ATP synthesis.

Structures of rotary ATP synthase from Thermus thermophilus during proton powered ATP synthesis | Science Advances www.science.org/doi/10.1126/...

This is a beautiful paper that relates the key idea of kinetic asymmetry, along with the possibility of slip and futile cycling, to understand the performance measures for synthetic molecular machines as well as other non-equilibrium processes coupled to chemical catalysis.

The physics of molecular machinery can be confusing. We developed a plug-and-play interactive tool based on trajectory thermodynamics. The user can now visualise how/why motors move directionally or respond to a force (see video), without knowledge of the physics or maths.
github.com/JoaquinBaixe...

Structure-performance relationships for catalysis-driven molecular machinery Molecular machine properties such as speed, efficiency, power, and torque depend upon particular features of structure, mechanism, and chemistry. The graphical abstract shows a Top Trumps-style repres...

Structure-performance relationships for catalysis-driven molecular machinery: Chem www.cell.com/chem/fulltex...

Interpreting molecular ratchets, directionality, and kinetic asymmetry through the work of Curie, Einstein, and Lewis - Communications Physics Scalar energy can drive formation of non-equilibrium steady-states or power directional processes such as chemotaxis, ion transport across a membrane, or walking along a polar track. This work focuses...

Interpreting molecular ratchets, directionality, and kinetic asymmetry through the work of Curie, Einstein, and Lewis
www.nature.com/articles/s42...

as an exercise it may be worth considering why the bar-hinge model would work fine when powered by light, but would not work when powered by a chemical reaction unless kinetic asymmetry were incorporated into the design.
www.nature.com/articles/s42...

A self-assembled nanoscale robotic arm controlled by electric fields An electrically driven DNA origami arm can exert piconewton forces and transport fluorophores and nanoparticles.

A self-assembled nanoscale robotic arm controlled by electric fields | Science www.science.org/doi/10.1126/...

The bar-hinge motor: a synthetic protein design exploiting conformational switching to achieve directional motility
doi.org/10.1088/1367...
via @ioppublishing.bsky.social

RNase H sequence preferences influence antisense oligonucleotide efficiency Abstract. RNase H cleaves RNA in RNA–DNA duplexes. It is present in all domains of life as well as in multiple viruses and is essential for mammalian devel

RNase H sequence preferences influence antisense oligonucleotide efficiency url: academic.oup.com/nar/article/...

Catalyst: Advancing nonequilibrium chemistry by clarifying misconceptions Yoshiyuki Kageyama obtained his PhD in multidisciplinary science from the University of Tokyo in 2006 and is currently an independent assistant profes…

Catalyst: Advancing nonequilibrium chemistry by clarifying misconceptions
www.sciencedirect.com/science/arti...