KruegerLab

Our new book "Self-Doubt" is written by scientists for scientists. You might think you are alone when you doubt your ability or suitability for science - you aren't. We all question ourselves and carry doubt with us daily.

www.amazon.co.uk/Self-Doubt-A...

Postdoc (m/f/d) in the field of T-Cell Biology

We are hiring! A fully-funded postdoc position in the field of T-cell biology is available immediately at our lab at JLU. Please check out the full ad here: www.uni-giessen.de/de/ueber-uns... or contact me for more details.

Congrats James! Well deserved!

We‘ll give it a go 😀

For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper?

You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...

Awesome performance of the band composed of great immunologists, including our Editor-in-Chief Matteo Iannacone behind the keyboard, at the #IUIS2025 Congress Party! 🎹🎸🎶

Steffen Jung announced Andreas Radbruch as keynote speaker at #IUIS2025, highlighting his strong support for European Immunology and the importance of backing EJI and European research!

Andreas presenting his newest EJI article by Schneider-Revueltas et al. onlinelibrary.wiley.com/doi/10.1002/...

Back from another great retreat to Schmitten, Taunus - this year with guests from @4r-rtg.bsky.social and from @jlugiessen.bsky.social and @mpi-hlr.bsky.social core facilities. We had inspiring method sessions on scientific diagrams & statistics, complexome proteomics, microscopy & image analysis ..

Congrats! I really hope that this approach to science will set an example for many.

Our research on human γδ T cells is in collaboration with the lab of David Vermijlen @ulbruxelles.bsky.social
u-cri.ulb.be/index.php?ac...
In this context, he is looking for a postdoc with experience in molecular biology. Apply now to become part of the team! 👇
euraxess.ec.europa.eu/jobs/306959

Thanks!

Awesome!

Have fun!

Thanks!

This was a joint effort of our lab spearheaded by Heike Kunze-Schumacher and @probertimmodels.bsky.social and great collaborators including @victorgreiff.bsky.social and Michael Meyer-Hermann. Thanks to @dfgpublic.bsky.social for their continuous support!

Taken together, we provide a framework to quantitatively determine cell cycle phase duration in vivo, which can be applied to a broad variety of biological systems.

We showed that thymocyte cell cycle phase progression is heterogeneous and that, in a model of irradiation induced involution followed by endogenous regeneration, cell cycle re-entry and G1 shortening promote thymus regeneration.

We discovered that a stretch model of simultaneous expansion or contraction of G1 and S phases explains steady-state thymocyte cell cycle speed transitions.

We combined sequential dual-nucleoside pulse labeling and DNA content analysis with two different agent-based mathematical models to map cell cycle phase durations in thymocytes in vivo.

High-resolution mapping of cell cycle dynamics during steady-state T cell development and regeneration in vivo Kunze-Schumacher et al. have developed an experimental and computational framework to generate a quantitative high-resolution map of cell cycle phase durations during murine T cell development. This map provides insight into how cell cycle phases are adjusted between slow- and fast-dividing populations at steady state and in a preclinical model of thymus regeneration.

A good start into 2025: www.cell.com/cell-reports.... High-resolution mapping of cell cycle dynamics during steady-state T cell development and regeneration in vivo.

Well. Perfect Q. Bullet proof glass yes. Jewish elementary school in Germany.

None of this would have been possible without the fun and productive collaboration with the Zarnack lab at Goethe University Frankfurt.
Thanks go out as well to our funders @dfgpublic.bsky.social (CRC902 and RTG2355).

Main conclusion: Functional analysis of miRNA binding sites in physiologically relevant context massively expands the pool of miRNA-mRNA interactions.

Prominent 3' binding in MREs exists and contributes to miRNA functionality in a complex manner.

We also uncovered a group of target sites associated with repeat regions in KZFP Zn-finger transcription factors, which behave differently. Located predominantly in the CDS, they act through translational repression.

There are many more binding sites than are predicted in silico.
While established targeting rules apply in general, we identified many functional non-canonical MREs.
We uncovered a prominent mismatch in the seed match, which retains functionality, thus extending the pool of targets substantially.

Using combined oligo-enriched eCLIP, RNAseq and ribosome profiling, we wanted to identify functionally relevant targets of miR-181. What did we learn?

It's finally out: https://academic.oup.com/nar/advance-article-abstract/doi/10.1093/nar/gkae416/7680629?utm_source=advanceaccess&utm_campaign=nar&utm_medium=email
Our map of miRNA miR-181 binding sites in thymocytes:

M.Sc. students Hanna and Marco from the Krueger lab

Congrats to Marco and Hanna, who received their M.Sc. diplomas! Excellent work by both of them!

ThymE 24 conference flyer

Thymus aficionados, mark your calendar for ThymE 24, the conference on T cell and thymus biology, May 27-31, 2024.
More information will follow soon.