Aldert Zomer

Really exciting that the preprint on Barbell, a new demultiplexer, is finally out!
It's the first tool that builds on Sassy, the approximate-DNA-searching tool that @rickbitloo.bsky.social and myself developed earlier this year, specifically with this application in mind.

Around 10% of your Nanopore reads (SQK-RBK114) are incorrectly trimmed. Here is why, and how our new tool Barbell solves it:

www.biorxiv.org/content/10.1...

Want to get started? github.com/rickbeeloo/b...

Congratulations Daniel! Amazing good news!

Consultancy for industry, payments for grant review etc . All goes to my group.

Just try to publish in society journals

I travel to or from schiphol almost weekly. It does happen sometimes. If you are in Utrecht and no train is coming, try to find the KLM cabin attendant and share a taxi :)

Professor or Associate professor (Tenure Track/Full) in Microbial Genetics of Health - Turun yliopisto - Työpaikat - Duunitori Avoin työpaikka: Professor or Associate professor (Tenure Track/Full) in Microbial Genetics of Health - Turun yliopisto, Turku. Duunitorilla lisäksi yli 30 000 muuta avointa työpaikkaa. Lue lisää nyt!

Professor (full) or Associate Professor (tenure track) in Microbial Genetics of Health (U. Turku Finland). Competitive starting package included! Please share.

duunitori.fi/tyopaikat/ty...

Come join us 2-4th March 2026 for a cracking #microbiome conference in Hinxton UK! Speaker line up is AMAZING and lots of opportunities for ECRs for selected talks, flash talks and posters.

Bursaries are also available!

See details below 👇

😂

Trying to come up with more titles I can put in a reply but failing

Indeed. All of the world was a slave to the music then

I don’t know Willem, maybe peak euro dance cannot be limited by a single song

Investigating pathogenicity in zoonotic Streptococcus suis at University of Cambridge on FindAPhD.com PhD Project - Investigating pathogenicity in zoonotic Streptococcus suis at University of Cambridge, listed on FindAPhD.com

www.findaphd.com/phds/project...

Maybe some it can be explained because we used diluted m17 (half m17) when we grew strains for molecular work. Lower salt , cells contain less salt therefore less chance of sparking (although the real reason was reducing costs) but I often wondered if that also made our electrotransformation better

I always cloned directly in L. lactis, most genes I was working with encoded proteins that E. coli did not like. I think I only used it to isolate empty vector from.

pORI28

That’s for pORI128 based plasmids . The integration ones without repA ( it’s been a while so I might be wrong)

I forgot if NisR was an activator or repressor. MG1363 might still be ok.

Plasmid-Based Gene Expression Systems for Lactic Acid Bacteria: A Review Lactic acid bacteria (LAB) play a very vital role in food production, preservation, and as probiotic agents. Some of these species can colonize and survive longer in the gastrointestinal tract (GIT), ...

Good overview pmc.ncbi.nlm.nih.gov/articles/PMC...

You might need nz9000 to prevent the nisin inducible promotor constantly driving expression of what you cloned.

pNG8048 perhaps?

I like the resfinder phenotype database which links a gene to potential resistance against a drug.

We are on the lookout for postdocs for two different projects at the intersection of ecology, evolution, and the human microbiome.

See thread for more information and reach out!

Don't miss the chance to be a part of this exciting conference on microbial typing & genomics #IMMEMXIV. The theme this year is Microbial typing: from fundamental to daily microbiology.

@escmid.bsky.social @esgem-sg.bsky.social

Register today and see you in Porto!
www.escmid.org/congress-eve...

Evaluating the impact of two decades of USAID interventions and projecting the effects of defunding on mortality up to 2030: a retrospective impact evaluation and forecasting analysis USAID funding has significantly contributed to the reduction in adult and child mortality across low-income and middle-income countries over the past two decades. Our estimates show that, unless the a...

Elon Musk casually, and illegally, dismantled USAID. According to a new study in the Lancet, that action will lead to *14 million* premature deaths between 2025 and 2030.

Again: Elon Musk is directly responsible for 14 million coming deaths. 4.5 million will be children.

A social card with an illustration of a bacteriophage and bacteria and the text "Research Scientist to join Dr Evelien Adrianssen's group to study bacteriophage, drug resistance and genetic evolution of Salmonella, Salary: 36,720 to 39,750, Contract length: 1.5 years, Apply by 2 July 2025

⏰ Closing soon! We’re looking for a Research Scientist to join Dr Evelien Adriaenssens's group to study bacteriophages, drug resistance and genetic evolution of Salmonella 🦠

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Tell me you’ve never been on a DB train without telling me you’ve never been on a DB train. There is a reason for the 1.4 score on trip advisor

.
When you've spend hours looking for your little black kitten...

. . . and then...

💙 🐾 💙

All use of antibiotics results in antibiotic resistance, not just incorrect use or overuse

Come join us in beautiful Lausanne! Deadline for applications: August 10th, 2025
wwwfbm.unil.ch/releve/appli...