Eliver Ghosn

#FlowLITE is a modular, easy-to-build, bead-based assay for characterizing & quantifying absolute number of antibodies in small volumes (~10μL) of any fluid specimen. This is Justin Hahm’s Honors Thesis project (Highest Honors!). He was mentored by PhD student Alex Nazzari, the co-first author.
2/4

Cell Press: STAR Protocols STAR Protocols is an open access, peer-reviewed journal from Cell Press. We offer structured, transparent, accessible, and repeatable step-by-step experimental and computational protocols from all are...

While #FlowLITE quantifies total antibodies, #FlowBEAT also quantifies antibody specificities to defined antigens. Both protocols can be adapted to your research needs.
4/4
star-protocols.cell.com/protocols/4572

Transient anti-interferon autoantibodies in the airways are associated with recovery from COVID-19 Nasal IgA1 autoantibodies against IFN-α are associated with better COVID-19 prognosis, including fewer symptoms and robust anti–SARS-CoV-2 immunity.

For context: FlowLITE was based on our previous technology, FlowBEAT, which we developed to measure human antibody isotypes against #SARSCoV2 and autoantigens in the #nasal #mucosa of #COVID19 patients (Sci Transl Med, 2024)
3/4
www.science.org/doi/10.1126/...

No more #ELISA assays?

Our 🌈 Annual Cook-off event keeps growing! We build community around great food, drinks, and music. Thank you for the delicious dishes and fantastic company.

The 2025 Chef Laureate goes to...

You will identify immune mechanisms underlying neuroinflammatory autoimmune diseases in children vs adults--autoimmune encephalitis (AE) and multiple sclerosis (MS). You will apply multi-omics single-cell assays to study the role of B/plasma cells and antibodies using patient samples (CSF and blood)

We are #hiring a postdoctoral fellow! Please share!

Join our B-cell team @Emory to lead NIH-funded projects on human neuro-immunology and autoimmunity in a co-mentorship and collaboration with neuroscientist Dr. Grace Gombolay.

Please apply here: faculty-emory.icims.com/jobs/156609/...
#BYourCell

You will identify immune mechanisms underlying neuroinflammatory autoimmune diseases in children vs adults--autoimmune encephalitis (AE) and multiple sclerosis (MS). You will apply multi-omics single-cell assays to study the role of B/plasma cells and antibodies using patient samples (CSF and blood)

We are #hiring a postdoctoral fellow! Please share!

Join our B-cell team @Emory to lead NIH-funded projects on human neuro-immunology and autoimmunity in a co-mentorship and collaboration with neuroscientist Dr. Grace Gombolay.

Please apply here: faculty-emory.icims.com/jobs/156609/...
#BYourCell

Main dish or Appetizer
1. Adas Polo & Lamb (Fatemeh Yousefian and Masoud Ilkhani)
2. Lasagna Bolognese (Alex Nazzari & Matt Stern)

Dessert
1. Mohanthal (Hardy Nariya)
2. Cesta di Frutta @katyely.bsky.social

No more #ELISA assays?

Our new FlowLITE protocol for simultaneously quantifying all (human and mouse) #antibody isotypes in a single tube using #flowcytometry is out in STAR Protocols: star-protocols.cell.com/protocols/4572

Is it time to retire the ELISAs for #antibody measurements?
1/4

Cell Press: STAR Protocols STAR Protocols is an open access, peer-reviewed journal from Cell Press. We offer structured, transparent, accessible, and repeatable step-by-step experimental and computational protocols from all are...

While #FlowLITE quantifies total antibodies, #FlowBEAT also quantifies antibody specificities to defined antigens. Both protocols can be adapted to your research needs.
4/4
star-protocols.cell.com/protocols/4572

Transient anti-interferon autoantibodies in the airways are associated with recovery from COVID-19 Nasal IgA1 autoantibodies against IFN-α are associated with better COVID-19 prognosis, including fewer symptoms and robust anti–SARS-CoV-2 immunity.

For context: FlowLITE was based on our previous technology, FlowBEAT, which we developed to measure human antibody isotypes against #SARSCoV2 and autoantigens in the #nasal #mucosa of #COVID19 patients (Sci Transl Med, 2024)
3/4
www.science.org/doi/10.1126/...

#FlowLITE is a modular, easy-to-build, bead-based assay for characterizing & quantifying absolute number of antibodies in small volumes (~10μL) of any fluid specimen. This is Justin Hahm’s Honors Thesis project (Highest Honors!). He was mentored by PhD student Alex Nazzari, the co-first author.
2/4

Our new FlowLITE protocol for simultaneously quantifying all (human and mouse) #antibody isotypes in a single tube using #flowcytometry is out in STAR Protocols: star-protocols.cell.com/protocols/4572

Is it time to retire the ELISAs for #antibody measurements?
1/4

Main dish or Appetizer
1. Adas Polo & Lamb (Fatemeh Yousefian and Masoud Ilkhani)
2. Lasagna Bolognese (Alex Nazzari & Matt Stern)

Dessert
1. Mohanthal (Hardy Nariya)
2. Cesta di Frutta @katyely.bsky.social

Our 🌈 Annual Cook-off event keeps growing! We build community around great food, drinks, and music. Thank you for the delicious dishes and fantastic company.

The 2025 Chef Laureate goes to...

It was great to present our lab's work "Engineering B-cell therapies for life-long immunity" at the Annual Research Day @emorydeptofmed.bsky.social

We discussed our Gates Foundation project and how studying human early-life B cells can inform this technology. Great discussions!
#25DOMResearchDay

It was great to present our lab's work "Engineering B-cell therapies for life-long immunity" at the Annual Research Day @emorydeptofmed.bsky.social

We discussed our Gates Foundation project and how studying human early-life B cells can inform this technology. Great discussions!
#25DOMResearchDay

What makes PhD students happy? Good supervision Supervisors who invest in positive mentoring relationships with their PhD candidates also reap the benefits for their own research.

What makes PhD students happy? Good supervision www.nature.com/articles/d41...

Establishing a Fixed Time Period of Admission and an Extension of Stay Procedure for Nonimmigrant Academic Students, Exchange Visitors, and Representatives of Foreign Information Media Unlike most nonimmigrant classifications, which are admitted for a fixed time period, aliens in the F (academic student), J (exchange visitor), and most I (representatives of foreign information media...

Comment period ends on Monday for a proposed rule change that would terminate student visas after 4 years. It also puts limits on exchange visitors and reps of foreign media, and shortens the length of time at the end of the visa from 60 days to 30 days.

www.federalregister.gov/documents/20...

Whether you think limiting student visas to 4 years is a good or bad thing, please comment. Be heard.

🚨 Immcantation.org user or interested in #Bcell repertoire analysis?
🗓️ Join the FREE virtual 2026 Immcantation Users Group Meeting
📅 Jan 22, 2026 | ⏰ 10AM–1:30PM ET
📢 Abstracts due Oct 1, 2025
🔗 immcantation.github.io/users-meeting/2026

#AIRRseq #Immcantation

For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper?

You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...

bioRxiv bat signal going up. We have a big backlog of submissions. If any affiliates have time to do some screening, we (and all the authors) would be immensely grateful! 🙏

Adoptively transferred Th17 cells cooperate with host B cells to achieve durable tumor immunity Cole et al. report that adoptively transferred, tumor-specific Th17 cells engage host B cells and drive B cell proliferation and differentiation. These Th17 cells require B cells for sustained immunit...

🧵1/ New in @Cancer_Cell: Our lab discovered that Th17 cells must team up with B cells to eliminate tumors, and prevent it from coming back.
🔗 Read: www.cell.com/cancer-cell/...
🧬This unexpected immune duo sparks tumor immunity.
🖼️Image: See schematic Th17/B cell antitumor action!

We are #hiring -- To clarify, this is for a second Postdoc position for the newly funded project.

We just received new funding to study human early-life B-cell and plasma cell immunity (including mucosal immunity to respiratory viral infections).

We are seeking a new researcher with expertise in B-cell biology to join our team! Send me a message!

We just received new funding to study human early-life B-cell and plasma cell immunity (including mucosal immunity to respiratory viral infections).

We are seeking a new researcher with expertise in B-cell biology to join our team! Send me a message!