Schematic of TN-seq in C. neoformans. Top left: Transposon insertions (orange arrow) into nonessential genes results in viable cells. In contrast, insertions into essential genes will result in dead and nonrecoverable cells. Top right: TN-seq works by generating a library of cells where each cell has a single independent transposon insertion in a random location. As in A, those insertions into essential regions cause the cells to die and are nonrecoverable. As a result, the total library (bottom) is depleted in insertions in essential regions. Bottom: The Ac/Ds transposon was split into an Ac transposase and a Ds transposon containing a neomycin resistance marker. This Ds transposon was integrated into an intron of URA5 and the Ac transposase was integrated into the safe haven locus. The resulting stain is ura− and neomycin resistant. Upon initiating transposition via growth on galactose, the strain becomes URA+ and mutant at another locus (depicted here as YFG1).
#Fungal infections are hard to treat due to #DrugResistance. @blakebillmyre.bsky.social &co use a high-throughput #TNseq system in #Cryptococcus neoformans to identify >1400 essential genes & reveal a role for #mitochondrial genes in #fluconazole sensitivity @plosbiology.org 🧪 plos.io/4dz3iVm
23.05.2025 10:15 — 👍 19 🔁 10 💬 1 📌 0
Deadly Fungi Are Here, and They’re Spreading
Unlike “The Last of Us,” virulent fungal disease isn't a work of science fiction.
Fascinating article about @normanvanrhijn.bsky.social work on the affects of climate change on fungi & fungal infections, with comments from MRC CMM's Elaine Bignell www.bloomberg.com/opinion/arti...
23.05.2025 09:13 — 👍 6 🔁 4 💬 0 📌 0
We are recruiting for a PhD student who wants to understand how bacterial membranes are built and how they function. It would be a biology meets maths project … if this piques your interest take a look here: macsys.org/monash-phd-s... for project details and contact info
20.05.2025 10:56 — 👍 14 🔁 14 💬 1 📌 1
Telomere bacteriophages are widespread and equip their bacterial hosts with potent interbacterial weapons
Klebsiella host strains infected with telomere phages can grow to be the dominant lineage in mixed populations.
"That telomere phages are so prevalent means that they are a selective force, one that we know little about. We now want to understand how the telomere-toxin is secreted and also understand how this ‘telocin’ wheedles its way into unsuspecting bacterial neighbors”
www.science.org/doi/10.1126/...
01.05.2025 22:04 — 👍 107 🔁 61 💬 7 📌 6
Lab head at the University of Melbourne. Studying the structure and biochemistry of microbial membrane proteins and metalloenzymes.
🇦🇺 Senior Lecturer at @sydneyuni.bsky.social and former NIHer. Research: Bacterial outer membrane biogenesis - membrane protein folding - protein translocation across membranes - novel antibiotics. He/him.
Microbiologist. Associate Professor at University of Illinois Chicago studying microbe-microbe interactions. Loves fungi and bacteria.
Associate editor at Nature Microbiology.
I read your microbiome papers!
All opinions are personal
🇩🇪🇸🇪🇮🇳
Fascinated by fungal growth & asymmetries. Institute of Biology Valrose, CNRS-Inserm-Université Côté d’Azur, Nice. Microscopy addict, all things cell biological
Researcher in Fungal Biology 🍄🔬. Currently Postdoctoral Researcher at @CBGP_Madrid 🇪🇸 Former MSCA IF Postdoc at @ibvnice 🇫🇷 PhD from @tuBraunschweig 🇩🇪
Microbial Evolution - AMR - University of Manchester
Research Fellow investigating #Candida and #Staphylococcus co-infections @mrccmm.bsky.social, Deputy Editor-in-Chief #AccessMicrobiology, Dr. rer. nat, 🇮🇪🇩🇪🇪🇺🇬🇧(she/her)
I study evolution in fungi using genomics at UC Riverside. He/him.
Tongva, Cahuilla, Serrano lands
http://lab.stajich.org/
PhD student at Trevor Lithgow's group Monash BDI. Facultative bioinformatician who in love with phages and their dark matters.
Head of Translational Parasitology in the Drug Discovery Unit at the University of Dundee. Portfolio leader for kinetoplastid drug discovery.
Passionate about developing new treatments for Neglected Tropical Diseases.
Pursuing my PhD in Microbiology at the University of Namur under Dr Regis Hallez.
Plant-soil microbes lecturer @FertiliserHub, @SciMelb. PhD @MonashUni on bacteria living on thin air. Alumni of @UNSWScience. She/her
Based at the University of Exeter, the MRC Centre for Medical Mycology is a research facility focussed on the global threat of fungal infections and anti-fungal resistance.
www.exeter.ac.uk/research/medicalmycology
www.thefungalthreat.com
The Australian Society for Biochemistry and Molecular Biology (ASBMB) aims to advance Biochemistry and Molecular Biology in Australia.
This year we are hosting the bi-annual ASBMB conference at the University of Queensland (29th September - 1st October)
The Mycological Society of America is a professional society for the study of fungi and fungal-like organisms & publishes the journal #Mycologia. #MSAfungi26
Assistant Professor @VU Amsterdam
Fungi, Ecology, Genetics, Microscopy, Symbiosis. #Scientist, #educator, #artist and #philosophy lover! 🌱🍄🔬👨🔬🎹🎵🏳️🌈
https://www.vasilis-kokkoris.com/
Evolutionary Biologist
Postdoc with Alejandro Couce @ UPM, Spain
Using Experimental Evolution to look at mutational biases in bacteria!
Formerly of Mike McDonald @ http://mcdonald-lab.com
Research Gate Profile: https://t.co/xhw63knBCj
ARC Future Fellow Macquarie University, School of Natural Sciences 🇦🇺 she/her | Functional 🧬,💊discovery, Synbio, 🐛, ♻️| Ex-@sangerinstitute @VertexPharma @Mlw_Programme
Professor at University of Zurich, Microbiology, Evolution, Ecology, Microbial Interactions, Infections
