Géraldine Laloux

Happy to finally show our story of the connection between nucleoid conformation, transcription & predation in our favorite predator Bdellovibrio bacteriovorus. 🧵 1/n

Experiments and analysis led by former post-doc @vrrenske.bsky.social in collaboration with @tunglejic.bsky.social & @rkoszul.bsky.social teams

Dynamics and interplay of gene expression and chromosome organization across a predatory lifecycle The obligate predatory bacterium Bdellovibrio bacteriovorus alternates between a motile attack phase and a growth phase inside another bacterium, during which it undergoes multiple rounds of DNA repli...

📝New preprint! #MicroSky

🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.

🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.

www.biorxiv.org/content/10.1...

🇪🇺 Smooth publishing experience with #microLife, the new @femsjournals.bsky.social journal of the European Academy for Microbiology.

🙏 @erc.europa.eu, F.R.S-FNRS & WEL-RI for support
4/4

🛠️This is the result of a long and careful work conducted by our research tech Charles de Pierpont, with help from PhD student Benoît Derneden and former lab member @remyophe.bsky.social, and validation by the whole team
3/4

🍝 Featuring the reversible spaghetti phenotype of #FtsZ -depleted Bdellovibrio: predator cell division is not required to exit the prey
2/4

Expanding the genetic toolbox of the obligate predatory bacterium Bdellovibrio bacteriovorus with inducible gene expression and CRISPR interference Inducible gene expression and CRISPR interference-mediated depletion in the predatory bacterium Bdellovibrio bacteriovorus.

🚨New paper out! #MicroSky
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
1/4

#MicroSky

Another great contribution to the “Cell Regulation” themed issue of Current Opinion in Microbiology www.sciencedirect.com/special-issu...

Out Of The Blue, It Came To Me

Inspiring “behind the paper” story here 👉 communities.springernature.com/posts/out-of... #MicroSky

Bacterial cell wall & periplasm paradigm shift - important read #MicroSky

www.nature.com/articles/s41...

Chromosome enthusiasts, York is calling!

Join us at The Biology & Physics of Chromosomes www.eventsforce.net/biochemsoc/f...

Late breaking abstract deadline
📆 7 August

@biophychrom.bsky.social @vslioy.bsky.social @damelab.bsky.social @graingerlab.bsky.social @biochemsoc.bsky.social #microsky

Thanks, Basile! All figures were drawn manually ✏️ 🖌️ by Coralie! @coralietesseur.bsky.social

Strategies and mechanisms of contact-dependent predation in bacteria Predatory bacteria kill other bacteria to acquire nutrients. Among diverse strategies to kill and feed on prey, contact-dependent predation stands out…

#MicroSky Summer read!

Predatory bacteria kill and eat other bacteria. Their deadly actions come in many flavors, often via direct physical contact between predator &
prey 🧛

How do they do it?
Our review 📖 @coralietesseur.bsky.social @ysantin.bsky.social

www.sciencedirect.com/science/arti...

Congrats to first author of both chapters @coralietesseur.bsky.social as well as @remyophe.bsky.social and @ysantin.bsky.social

a man wearing sunglasses and a white t-shirt is holding something in his hands . ALT: a man wearing sunglasses and a white t-shirt is holding something in his hands .

We wrote 2 📕 chapters with tips & tricks to identify protein-protein interactions in bacteria 🦠

1) In vivo proximity labeling with miniTurbo link.springer.com/protocol/10....

2) An adapted polar recruitment assay feat. E. coli as a test tube link.springer.com/protocol/10.....

Enjoy #MicroSky 🧪

Please repost: PhD position with my lab and @weisslab.bsky.social in a great collaboration on bacterial adhesion #microsky. See drive.google.com/file/d/1GJD4...

#MicroSky

A bacterial CARD-NLR immune system controls the release of gene transfer agents Bacteria have evolved a wide array of immune systems to detect and defend against external threats including mobile genetic elements (MGEs) such as bacteriophages, plasmids, and transposons. MGEs are ...

1/ Excited to share our latest work on gene transfer agents (GTAs) in Caulobacter crescentus, led by Emma Banks in collaboration with Pavol Bardy and Mai Nguyen from York!!! See a brief thread below.
shorturl.at/o6S1w

#Postdoc position - at the University of Marburg (Germany), funded within our ERC Advanced Grant "C-SWITCH"

Focus: CTP-dependent molecular switches as a new principle of cellular regulation in bacteria (including bacteriophages)

Apply by 04 May: stellenangebote.uni-marburg.de/jobposting/2...

I’m very happy to share our latest work on the ParABS system. We investigated the dynamic interaction between the ATPase ParA and the CTPase ParB mediating chromosome segregation in Myxococcus xanthus. I would like to thank all the people involved.

Please repost: Postdoc position in antibiotic discovery in a large multidisciplinary project combining high-throughput approaches, microscopy, AI, and hit-to-lead optimization at @umontreal.ca and @mila-quebec.bsky.social. Apply to ML.Antibiotics@gmail.com. See
lnkd.in/dVVk4N3s. #microsky

Future Directions of the Prokaryotic Chromosome Field In September 2023, the Biology and Physics of Prokaryotic Chromosomes meeting ran at the Lorentz Center in Leiden, The Netherlands. As part of the workshop, those in attendance developed a series of ....

In @molecularmicro.bsky.social: two special issues on Prokaryotic Chromosome Organization. Many excellent contributions, including a vision article: 'Future directions of the Prokaryotic Chromosome Field', outcome of BioPhyChrom 2023 @biophychrom.bsky.social onlinelibrary.wiley.com/doi/10.1111/...

A million thanks to the ERC for the opportunity to explore bacterial predatory attacks and cell cycle switches with our VAMPIRE Consolidator grant @deDuveInstitute @UCLouvain_be @frsFNRS 🦠 🔬 🧛

2 days left. Asst/Assoc Prof position in bacteriology, Université de Montréal. Deadline ***Nov. 15*** come join our growing community of bacteriologists. BTW Canada is the USA’s Bluesky!

New job offer: we search a postdoc (bioinformatics) to work on mobile genetic elements and gene flow in bacteria, with a focus on antibiotic resistance within a consortium including bioinformatics, synthetic biology, epidemiology & biochemistry. See research.pasteur.fr/en/job/postd... #MicroSky

Former PhD student Ophélie Remy and I are cell poles enthusiasts, so we were delighted to offer a brief perspective on bacterial polarity for the Special Issue on Cell and tissue polarity in the Journal of Cell Science. journals.biologists.com/jcs/article-.... #MicroSky

#MicroSky

Our last story on cell polarity in Bdellovibrio bacteriovorus is now published in Journal of Bacteriology! Feat. a protein that marks the "head" of future daughter cells before non-binary division, a new DivIVA pattern, and 1st use of proximity labeling in Bdello!
journals.asm.org/doi/10.1128/...

Archaeal and bacterial cell division: Beyond the Z-ring The objective of this EMBO Workshop is to discuss advances in prokaryotic cell division. Most archaea and bacteria divide using a ring-structure formed by the tubulin homologue FtsZ, the Z-ring. Rese�...

cell divisionist take note 👇

meetings.embo.org/event/24-z-r...