Alejandro Melero

Extremely grateful to @hfspo.bsky.social and super excited to set this project in motion together with the incredibly talented @ritamateus.bsky.social and @vmonje.bsky.social Zebrafish hide a secret: lipid membranes shaping the crystals behind their stunning structural colors. How? Stay tuned! #HFSP

Next, work from the Etienne Herzog, @davip-bdx.bsky.social and @fronzeslab.bsky.social using cryo-CLEM and cryo-ET of cortico-striatal dopaminergic synaptosomes to delineate the architecture of non-synaptic modulation: www.biorxiv.org/content/10.1...

This goes beyond my wildest imaginations! Congratulations to @mathilda95.bsky.social @nadlerlab.bsky.social and all the authors! Such a big step forward for membrane biology.

Looking for a PostDoc? Exciting science and great city! Join us @albertpol.bsky.social @albertherms.bsky.social in @idibaps.bsky.social Barcelona to work in an @ercresearch.bsky.social funded project to study how lipid droplets attract and kill bacteria. www.clinicbarcelona.org/uploads/medi...

Check it out, is finally out!!

Exciting news! #MembraneTrafficking online seminars with @felixmendu.bsky.social @franbottanelli.bsky.social and me are back!
Check the spectacular Jan-April 2025 lineup.
Details (Zoom links, calendar invites) on the mailing list, Slack group & website: felixcampelo.wixsite.com/membranetraf...

Graphical abstract of the Organelle Profiling paper, showing the workflow: Endogenously tagged organelle library; Native IP proteomics; Data-driven graph clustering; resulting in a subcellular proteome map with more than 7,600 proteins localized across 19 subcellular compartments.

Time to share our organelle proteome paper
out in @cellcellpress.bsky.social: doi.org/nzwz
We created a spatial map of human cells using organelle immunoprecipitation at scale.
Locate your fav protein among the 7,600 we mapped across 19 subcellular compartments: organelles.sf.czbiohub.org/

1/3 🧪

Any #SpatialTranscriptomics people out there interested in studying #microglial development?
We need a collaborator based in the Americas or Asia to apply for a #HSPF grant

www.hfsp.org/funding/hfsp...

"A study of federally funded research projects in the United States estimated that principal investigators spend on average about 45% of their time on administrative activities related to applying for and managing projects rather than conducting active research"

www.pnas.org/doi/10.1073/...

Happy to share our Perspective on #microglia #pruning and help to answer the questions of whether they are hunters or gatherers of brain #synapses 🧠🧪

#neuroskyence
nature.com/articles/s4159…

Postdoc position available in the Röper lab at the University of Cambridge @PDN_Cambridge!
Join us and discover the role that control of cytoskeletal dynamics, crosstalk and cell adhesion play in the formation of the nephron tube in human renal organoids!
jobs.cam.ac.uk/job/48947/

Kicking off here with some old but gold movies showing ER-lysosome interactions triggered by blue-light excitation of the light-sensitive protein eMags. A shining example of how light can control cellular dynamics! 💡🧫✨

Stunning paper by Anne Simonsen's lab, identifying BEACH domain-containing proteins as membrane coat proteins involved in endosomal sorting: rupress.org/jcb/article-...

Read also @franbottanelli.bsky.social's beauty on ARF1 directing cargo maturation into RE!(www.nature.com/articles/s41...)

A fun journey of full-length EGFR with Dan Leahy and
@edwardmarcotte.bsky.social at UT Austin @utaustin.bsky.social. See below:
Structure and organization of full-length Epidermal Growth Factor Receptor in extracellular vesicles by cryo-electron tomography www.biorxiv.org/content/10.1...

Cryo-electron tomography reveals how COPII assembles on cargo-containing membranes - Nature Structural & Molecular Biology The authors reconstituted coat protein complex II vesicles from native membranes and used cryo-electron tomography and subtomogram averaging to reveal the arrangement of inner and outer coat layers on...

Latest paper from our lab out now! #TeamTomo #CryoET

www.nature.com/articles/s41...

Imaging interorganelle contacts at a glance poster Maria Clara Zanellati, Chih-Hsuan Hsu and Sarah Cohen

- Imaging interorganelle contacts at a glance
- Reviews on machine learning, synchrotron X-ray imaging, fluorescence polarisation microscopy and imaging cell-cell junctions
- An Editorial from FocalPlane
journals.biologists.com/jcs/issue/13...

Characterizing heterogeneity in amyloid formation processes pubmed.ncbi.nlm.nih.gov/39566372/

Finally this cool story by @brfalcon.bsky.social is out! 😍😍

Deep proteomic analysis of microglia reveals fundamental biological differences between model systems Lloyd et al. generated a comprehensive microglia proteome database, identifying over 11,000 proteins. Fundamental differences between ex vivo and in vitro microglia associated with protein synthesis, ...

My latest paper, deep proteomic analysis of mouse and human microglia: www.cell.com/cell-reports...

How do organelles fit together inside a crowded cell? Answering this question requires a way to image many organelles in a whole cell at high resolution with high throughput. Our new preprint shows how to do this in yeast using soft x-ray tomography
www.biorxiv.org/content/10.1...