Dolf Weijers

High resolution, proteome-wide mapping of subcellular protein localization in plants https://www.biorxiv.org/content/10.64898/2026.02.27.708449v1

This work was led by PhD candidate Monique van Schie and proteomics wizard Mark Roosjen in our team.

We welcome any feedback to this work!

(9/9)

tinyurl.com/Root-map
tinyurl.com/Seedling-map
tinyurl.com/Marpol-map
tinyurl.com/BFA-maps
tinyurl.com/gnom-maps

(8/9)

We hope that these maps will enable more protein-centred approaches in the plant sciences field, and many hypotheses to be generated. To enable this, we developed 5 Shiny apps (links in next post) to navigate the data.

(7/9)

We next compared spatial proteomes in the gnom mutant (BFA target) and wild-type, and identify a small set of proteins that move both after BFA treatment and in the gnom mutant. These include several well-known targets.

This shows the power of global dynamic subcellular proteome mapping

(6/9)

Where the maps are truly insightful is in the dynamic comparisons of global protein localization between conditions. We did this for a brief BFA treatment, and identify >800 proteins that change location. Most of these were not previously connected to BFA or trafficking.

(5/9)

The maps are a reference on their own, and now offer expertimentally defined subcellular localizations for thousands of proteins where this was not known. They can also be integrated with other proteomics datasets, as we show for targets and interactors of the BIN2 protein kinase

(4/9)

Comparing maps and localizations between orthologs in Arabidopsis and Marchantia shows that subcellular protein localization is strongly conserved, but also identifies a few hundred proteins whose localization diverged. These are enriched in membrane trafficking pathways.

(3/9)

Using fluorescently tagged lines, we show that >80% of these patterns check out, which is probably an underestimation.
We generated reference maps in Arabidopsis roots, Arabidopsis seedlings and Marchantia thallus, the latter also valildated with fluorescent markers.

(2/9)

We are very excited to share a new resource from our team: spatial subcellular proteome maps in plants! We developed an MS-based method that registers localizations of about 8000 proteins in Arabidopsis roots in a single experiment.

(1/9)
www.biorxiv.org/cgi/content/...

Congrats, Cris!!!

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Vwry good read, and inspiring words from a phenomenal colleague 🤩

Congrats on this amazing work!!!

Congrats MCC!

Highly recommended! I had the pleasure to speak at an earlier edition and found it an amazing gathering!

Integrative proteomics approaches for data-driven reconstruction of kinase signaling.

Masters of perception: phosphorylation-dependent signaling in plants

#TansleyInsight by Roosjen et al.

nph.onlinelibrary.wiley.com/doi/10.1111/...

#plantscience

Two more weeks to apply - come join us () in The Netherlands as a postdoctoral researcher on large-scale metagenomic exploration of new lineages!

Registration is OPEN for the 2026 Santa Cruz Meeting on Developmental Biology!!! Please spread the word!

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Full Professor and Chair Laboratory of Biophysics We are looking for a Full Professor in the Biophysics chair group, part of Wageningen University & Research (WUR)

The Laboratory of Biophysics at Wageningen University & Research (NL) is looking for a new chair! Details here: www.wur.nl/en/vacancy/f...

Full Professor and Chair Laboratory of Biophysics We are looking for a Full Professor in the Biophysics chair group, part of Wageningen University & Research (WUR)

www.wur.nl/en/vacancy/f...

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Activities - GreenTE Activities Webinars ”MechanoBioTalks – Mechanics, Materials and Living Matter” is a webinar series supported by the GreenTE consortium and organized […]

A new webinar series is born - ''MechanoBioTalks - Mechanics, Materials and Living Matter", to bring together and inspire researchers interested in biomechanics, biophysics and biology across different kingdoms of life. Join us!

For info and registration visit green-te.nl/activities/

Don’t miss this! The young researchers in our GreenTE consortium initated a webinar series on Plant Mechanobiology. Amazing speakers, free of charge, what is there NOT to like…?

Save the date: Plant Development PhD school (i.e. Retzbach 2.0) in Neustadt, Germany.

Sept 23rd-25th, 2026
~€350 all inclusive

Excellent speakers and a relaxed atmosphere for early-career researchers (PhDs & postdocs). Registration opens soon.

For more info, see
raissiglab.org/plantdevosch...

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🏅 Celebration of the Klaus Sander, Hilde Mangold, and José Campos-Ortega Awards 🏅

👉 www.uni-potsdam.de/en/gfe-meeti...

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