Holly Rucker's Avatar

Holly Rucker

@hollyrucker.bsky.social

PhD candidate at UW-Madison // interested in astrobiology, geobiology, isotopes, and Precambrian Earth

81 Followers  |  97 Following  |  25 Posts  |  Joined: 06.09.2023  |  1.8633

Latest posts by hollyrucker.bsky.social on Bluesky

Has anyone here used stoggles and liked them? I would love to not have to switch between my safety glasses and regular glasses constantly in lab but they are a bit pricey πŸ₯½

07.11.2025 21:35 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

πŸŽ‰ Huge congrats to Holly Rucker for winning First Place in the Midwest Geobiology Conference Best Oral Presentation! πŸ† Amazing work, Holly! @uwmadscience.bsky.social @uwbact.bsky.social @hollyrucker.bsky.social #UWMad

31.10.2025 18:45 β€” πŸ‘ 2    πŸ” 1    πŸ’¬ 0    πŸ“Œ 0
Image with a blue to purple gradient in the background with graphics of a planet, sunset on water, DNA & an Erlenmeyer flask, with text "WiCOR seeks a Postdoctoral Fellow, Apply by Nov 15, Learn more at wicor.wisc.edu/postdoc"

Image with a blue to purple gradient in the background with graphics of a planet, sunset on water, DNA & an Erlenmeyer flask, with text "WiCOR seeks a Postdoctoral Fellow, Apply by Nov 15, Learn more at wicor.wisc.edu/postdoc"

WiCOR seeks a #postdoctoral fellow to participate in Center #research in any discipline related to our two main research questions:
1. How do habitable planets form? πŸͺ
2. How does life emerge on habitable planets? 🧬

Full information at: wicor.wisc.edu/postdoc/

Apply by Nov 15

πŸ”­ πŸ§ͺ
#postdoc #hiring

27.10.2025 16:19 β€” πŸ‘ 8    πŸ” 3    πŸ’¬ 0    πŸ“Œ 2
Post image

currently reading: The Sea Around Us

I somehow have never read any of Rachel Carson’s books so I am making up for that now!

11.10.2025 17:38 β€” πŸ‘ 2    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

I had a great time being interviewed for this!

10.10.2025 21:56 β€” πŸ‘ 4    πŸ” 2    πŸ’¬ 0    πŸ“Œ 0
Post image Post image

currently reading Strata by Laura Poppick-looking forward to her talk for the WI Book Festival!

03.10.2025 22:27 β€” πŸ‘ 2    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
Post image

Happy national dog day from Yuki β™₯️

26.08.2025 15:55 β€” πŸ‘ 1    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

Finished some updates to the gas manifold I’ve been building and turned it on for the first time today- it works great πŸ₯ΉπŸ™ŒπŸ»

09.07.2025 21:59 β€” πŸ‘ 8    πŸ” 1    πŸ’¬ 0    πŸ“Œ 0
Microbial N2O reduction in sulfidic waters: Implications for Proterozoic oceans Throughout Earth’s history, shifts in ocean redox influenced the bioavailability of trace metals, shaping the activity of microorganisms. In Proterozoic oceans, the precipitation of copper (Cu) with sulfide was hypothesized to limit the bioavailability of Cu. This limitation may have suppressed microbial reduction of nitrous oxide (N2O), due to the Cu dependency of nitrous oxide reductase (Nos). It is thought that without this critical microbial sink, Proterozoic oceans were a significant net source of N2O. Here, we revisit this paradigm in light of recently derived ∼20-fold lower estimates for sulfide in Proterozoic seawater and an empirical evaluation of the potential for microbial N2O reduction under sulfidic conditions. Leveraging publicly available environmental metatranscriptomes, we infer active N2O reduction from the detection of nosZ transcripts in multiple marine and lacustrine systems in which sulfide and Cu concentrations are analogous to those of the Proterozoic. In controlled culture experiments, we demonstrate that the purple non-sulfur bacterium Rhodopseudomonas palustris can reduce N2O at sulfide concentrations up to 100 Β΅M, well above levels predicted for Proterozoic oceans. Based on trace metal speciation modeling, we suggest that Cu remains bioavailable under Proterozoic-like conditions as a dissolved CuHS complex. Using phylogenetics, we infer that early N2O reducers were probably anoxygenic phototrophs and performed N2O reduction as dark metabolism. Collectively, these observations suggest microbial N2O reduction occurs under euxinic conditions, implying that Proterozoic marine N2O emissions were substantially lower than previously proposed. Our conclusions inform our understanding of the microbial ecology in sulfidic waters, the early climate, and the search for extraterrestrial life. ### Competing Interest Statement The authors have declared no competing interest. National Aeronautics and Space Administration, https://ror.org/027ka1x80, 80NSSC17K0296

New preprint on ancient oceans!

We challenge the idea of early Earth as major N2O source; reshaping views on climate & biosignatures. Led by S. Buessecker @annedekas.bsky.social lab!

➑️ Microbial N2O reduction in sulfidic waters: Implications for Proterozoic oceans
www.biorxiv.org/content/10.1...

07.07.2025 16:31 β€” πŸ‘ 15    πŸ” 7    πŸ’¬ 0    πŸ“Œ 0

I feel like Zotero’s upload feature only successfully copies over the citation info about 50% of the time πŸ˜“

30.06.2025 21:53 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
Post image Post image

Been analyzing vacuΓ±a bone, tooth (left) and collagen (right) for C/N/O isotopes this week at IsoCamp! @isocampunm.bsky.social

21.06.2025 18:41 β€” πŸ‘ 3    πŸ” 1    πŸ’¬ 0    πŸ“Œ 0
Post image

Added some amazing prints by @drlperezdiaz.bsky.social to the lab!

13.06.2025 15:53 β€” πŸ‘ 8    πŸ” 2    πŸ’¬ 1    πŸ“Œ 0
Post image Post image

Had my third year committee meeting yesterday (went great!) so I made a cannoli bundt cake to celebrate 🍰 not the best photos but it tasted amazing

05.06.2025 16:30 β€” πŸ‘ 1    πŸ” 1    πŸ’¬ 0    πŸ“Œ 0
iron toxicity model

iron toxicity model

New preprint: eukaryotic origins! πŸ₯

We propose that iron -not oxygen- may have delayed the rise of complex life. Our model reframes the β€œoxygen delay” puzzle: the gap between atmospheric O2 and early eukaryotic fossils may reflect iron-mediated stress, not for O2:

www.biorxiv.org/content/10.1...

02.06.2025 18:19 β€” πŸ‘ 47    πŸ” 19    πŸ’¬ 2    πŸ“Œ 3

🚨 I’m hiring a postdoc for Fall 2025 with experience in microbial β€˜omics data analysis and phylogenetics/genomics. Projects will be centered around ancient microbial ecology & evolution. Click here for more information: bpb-us-e1.wpmucdn.com/wp.txstate.e...

Please share, thank you πŸ™πŸ»

28.05.2025 15:17 β€” πŸ‘ 41    πŸ” 46    πŸ’¬ 1    πŸ“Œ 2

Excited to share what I’ve been working on for the first part of my PhD!

27.05.2025 20:46 β€” πŸ‘ 4    πŸ” 2    πŸ’¬ 0    πŸ“Œ 0
An acrylic painting by Jim Musil depicting a farm in Wisconsin. A red barn sits at the end of a dirt road in the middle of a verdant green field. A colorful sky dominates the composition.

An acrylic painting by Jim Musil depicting a farm in Wisconsin. A red barn sits at the end of a dirt road in the middle of a verdant green field. A colorful sky dominates the composition.

My painting WISCONSIN FARM

26.05.2025 17:35 β€” πŸ‘ 13773    πŸ” 1064    πŸ’¬ 297    πŸ“Œ 40
Post image Post image

a few more pictures before I leave πŸ₯²

24.05.2025 04:36 β€” πŸ‘ 1    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
Post image Post image

Had a wonderful time at the Geobiology conference and in Banff! Kept thinking of the quote β€œstanding on the shoulders of giants” while looking out into the mountains & thinking about the Burgess Shale. Feeling very inspired to jump back into work! πŸ§¬πŸ‘©πŸ»β€πŸ”¬

24.05.2025 02:02 β€” πŸ‘ 5    πŸ” 1    πŸ’¬ 1    πŸ“Œ 0

Thank you to the conference organizers for this award! Having a great time in Banff this week πŸ˜„

22.05.2025 14:52 β€” πŸ‘ 7    πŸ” 1    πŸ’¬ 0    πŸ“Œ 0
Preview
Ecological constraints and evolutionary trade-offs shape nitrogen fixation across habitats Life s transition into new habitats has profoundly shaped its trajectory through a reciprocal relationship with Earth s changing environments. To understand how ancient metabolisms co-evolved with the...

πŸ“£ New today! Morgan Sobol et al. show how nitrogen fixation networks co-evolved with Earth's changing environments, past & present. 🌍🧬

Beautiful combination of microbial networks, evolutionary trade-offs & ecological feedbacks.

Congrats, @msobol.bsky.social! πŸ‘ www.biorxiv.org/content/10.1...

21.05.2025 16:15 β€” πŸ‘ 11    πŸ” 7    πŸ’¬ 1    πŸ“Œ 0
Post image

Excited to share that our lab members Aya Klos, Holly Rucker @hollyrucker.bsky.social and Zach Adam will be presenting their work at the Banff Geobiology Meeting next week!

βœ… Ancient enzymes
βœ… Eukaryotic origins
βœ… Geobiological deep time

See you there, eh? πŸ‡¨πŸ‡¦
cms.eas.ualberta.ca/geobiology20...

16.05.2025 22:24 β€” πŸ‘ 3    πŸ” 2    πŸ’¬ 0    πŸ“Œ 0
Post image Post image

Huge congrats to Holly Rucker @hollyrucker.bsky.social on winning the UW–Madison Bacteriology Ph.D. Student Excellence Award! We're so proud of you, Holly, well deserved! @uwbact.bsky.social @uwmadscience.bsky.social #SupportScience #GoHolly πŸš€πŸš€πŸš€

02.05.2025 21:17 β€” πŸ‘ 10    πŸ” 2    πŸ’¬ 2    πŸ“Œ 0
Post image

It’s National Pet Day and I can finally participate! We adopted Yuki back in October and I’m so happy to have her β™₯️

11.04.2025 23:00 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
Post image

🚨New preprint alert!🚨

We mapped Mo-dependent pathways across microbes and show this reliance emerged before oxygen showed up. Our results challenge assumptions about Mo scarcity on early Earth. Study led by Aya Klos!

Biology’s been metal since the very beginning🀘

www.biorxiv.org/content/10.1...

07.04.2025 18:25 β€” πŸ‘ 56    πŸ” 21    πŸ’¬ 1    πŸ“Œ 4
Post image

🚨New paper!🚨

A comprehensive take on the origins and evolution of translation factors & how these essential players evolved across the tree of life. 🌍🧬

Led by Evrim Fer @uwmadisonmdtp.bsky.social grad student! πŸ‘

Free access: www.sciencedirect.com/science/arti... @cp-trendsgenetics.bsky.social

24.03.2025 14:46 β€” πŸ‘ 94    πŸ” 46    πŸ’¬ 3    πŸ“Œ 4
Keck Foundation grant funds UW–Madison research to explore an enzyme behind the origins and early evolution of life on Earth | Research | UW–Madison searchopen menucloseExpandCollapseExpandCollap...

🚨 We're hiring! 🚨

Our lab is looking for a lab technician & a postdoc to study enzyme evolution across geologic time.

Learn more about one of our projects here: ⬇️
πŸ”— research.wisc.edu/uncategorize...

Folks who recently lost jobs are especially encouraged to apply! #Hiring #ScienceJobs

15.02.2025 23:27 β€” πŸ‘ 14    πŸ” 15    πŸ’¬ 1    πŸ“Œ 0
Post image

πŸš€ We’ve just given our lab website a fresh new look! We plan to update it frequently. Come take a look! πŸ”¬πŸ‘‡
www.kacarlab.org

27.01.2025 17:23 β€” πŸ‘ 6    πŸ” 1    πŸ’¬ 1    πŸ“Œ 0

Photo by dmnx(.)photo on insta!

24.01.2025 17:47 β€” πŸ‘ 0    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
Post image

Got an action shot of me talking about how Hallucigenia & the Cambrian terrify me during my comedy science talk last week 🦠πŸͺ±

24.01.2025 17:46 β€” πŸ‘ 2    πŸ” 1    πŸ’¬ 1    πŸ“Œ 0

@hollyrucker is following 20 prominent accounts