Alexandre Mayran

It's always special when you find your own paper in the table of contents of the journal 😍

"The cis-regulatory logic integrating spatial and temporal patterning in the vertebrate neural tube" -> Now out in its final form

www.cell.com/developmenta...

Thrilled to share our new review in Current Opinion in Genetics & Development on TE driven innovation in gene regulation🤘. I am honored to be part of this with two major TE aficionados @cedricfeschotte.bsky.social and @trono-lab.bsky.social
#TEsky #TEworldwide

authors.elsevier.com/c/1m6MC,LqAZ...

Stay tuned for other announcements to come soon @institutcochin.bsky.social 12/12

Major thanks to all the funding agencies, @snf-fns.ch , #NRP79, EPFL, @hfspo.bsky.social , and to the three reviewers for their constructive comments helping improve the manuscript. 11/12

Finally, a big thank for all co-authors: Dominique Kolly, Lucille Lopez Delisle, @yuliia-romaniuk.bsky.social, @maxineleonardi.bsky.social , Anne Catherin Cossy, Theo Lacroix, @hocinerekaik.bsky.social , Ana Rita Amândio, @pierreosteil.bsky.social , and @denisduboule.bsky.social. 10/12

Our data supports that the interaction of somitic cells with N-Cadherin mutant cells of the same identity can be sufficient to drive epithelialization, bypassing both Matrigel in vitro or Surface ectoderm ex vivo requirement. 9/12

Interestingly, this is reminiscent of a study in 2000, where Correia and Conlon reported (www.sciencedirect.com/science/arti...) that tail bud explants only form epithelial somite when both mesoderm and surface ectoderm are present. 8/12

Surprisingly, we found that WT cells in the anterior portion establish an apico-basal polarity, with the basal side facing N-Cadherin mutant cells similarly to what Matrigel would normally cause. 7/12

This coherent organization even leads to the formation of polarized somitic tissue, despite the WT cells normally requiring Matrigel for this. 6/12

How are these organized? N-Cadherin expressing cells are dispersed along the entire A-P axis forming a network of connected cells. However, tissue organization is much more coherent, suggesting that cell fate organization can dominate over N-Cadherin expression. 5/12

We generated mosaic gastruloids with varying proportion of mutant cells to force differential N-Cadherin expression. Interestingly, the elongation was directly correlated to the proportion of mutant cells, linking gastruloid elongation to cell adhesion. 4/12

However, unlike TLS which rely on Matrigel, this occurs without any epithelialization. 3/12

Here, we could confirm that gastruloids lacking N-Cadherin self-organize correctly, in fact even better than their wildtype counter part as they form polarized somitic tissue without requiring exogeneous extra cellular matrix such as matrigel. 2/12

Cadherins modulate the self-organizing potential of pseudo-embryos Gastruloids derived from ES cells mimic embryonic patterning through robust self-organization. Mayran et al. uncover that this competence depends on a cadherin switch regulated by Snai1-driven E-cadhe...

Happy to present the final version of our Cadherin and gastruloid manuscript, online at @cp-cellreports.bsky.social.
www.cell.com/cell-reports...
Initially in BiorXiv (www.biorxiv.org/content/10.1...) 1/12

And we have another open position, this time with a focus on Genome Biology! Join a great community in Vienna to bring your research to the next level!

Spread the word! There is a professor position opening at our University @upcite.bsky.social. There are several possible (great) labs to join, but if you are interested in DNA methylation and epigenetics in mammals, don't hesitate to contact me directly!

Really excited to present the results of a fantastic collaboration with Jesse Veenvliet @jesseveenvliet.bsky.social @mpi-cbg.de @poldresden.bsky.social 🤩

We find a unique mechanism for body axis elongation in mammals, different from other vertebrate species

➡️ www.biorxiv.org/content/10.1...

thanks for sharing this tool. I was all the more impressed that I also gave it 'non' biological papers : it found very technical gaps. It also scans and interprets the figures, and all of this is done very quickly. I honestly think this approach is going to revolutionize our way to deal with papers.

Dissecting the impact of transcription factor dose on cell reprogramming heterogeneity using scTF-seq - Nature Genetics This study introduces single-cell transcription factor (TF) sequencing, a single-cell barcoded and doxycycline-inducible TF overexpression approach that reveals dose-sensitive functional classes of TFs and cellular heterogeneity by mapping TF dose-dependent transcriptomic changes during the reprogramming of mouse embryonic multipotent stromal cells.

🧵1/ Excited to share our new paper introducing a new #singlecell assay: scTF-seq, a high-throughput single-cell approach to explore how transcription factor (TF) dose shapes cell identity and reprogramming outcomes. 🔗 www.nature.com/articles/s41... Big congrats to the entire team @EPFL & @SIAT_China

In this beautiful system, we could show uncoupling of gene expression profiles, morphology and patterning… #NotInTheGenes 😉 (also includes a method to do long term 🔬 of elongating gastruloids)
Thank you to all involved @jeromewongng.bsky.social @thomasgregor.bsky.social @alexandremayran.bsky.social

L’art délicat de faire taire les gènes Le développement embryonnaire ne requiert pas uniquement l’activation des gènes, mais également une régulation fine de leur répression.

#ResultatScientifique 🔎| Faire taire les gènes est aussi crucial que les activer : le répresseur Snail révèle son rôle essentiel dans le développement embryonnaire 🧬
✍️ @laghalab.bsky.social et Ovidiu Radulescu
📕 @natcomms.nature.com | buff.ly/pmtzA1t

Fantastic work congrats to you all, it's been great to see this story being build over the years

hoxd13a reporter in a zebrafish larva

Are digits modified fins, or evolutionary innovations? Read how we tackled this old question from a new angle🧪
A story with @chasebolt.bsky.social, @homeobox.bsky.social and myself, coordinated by @denisduboule.bsky.social from @college-de-france.fr and published in @nature.com today!
#InHoxWeTrust

Out today. 🙏 again to everyone for this wonderful piece of work, in particular to Aurelie @aurhin.bsky.social Chase @chasebolt.bsky.social and Brent @homeobox.bsky.social. 🙏 also to the Harris lab @fish4walking.bsky.social and @neilshubin.bsky.social @biology-unige.bsky.social @college-de-france.fr

Bioinformatician / Data Scientist (m/f/d) Bioinformatician / Data scientist position in the Aztekin Lab focusing on Structural Regeneration and support the working group together with the other members

🚀 Looking for a Bioinformatician / Data Scientist to join our crew and explore regeneration through single-cell genomics & evolutionary biology! @mpi-bio-fml.bsky.social
📩 Questions? Contact us ✉️
🔗 Apply here: www.mpg.de/25243280/bio...

Similar huge trend at last round of @erc.europa.eu grant application. @ec.europa.eu must adapt to this trend!

Pleased to announce launching Institut Cochin Foundation, on Sept. 18 at 6 p.m., upon registration, either in the Rosalind Franklin room, 22 rue Méchain, Paris or via video conference: presentation of @institutcochin.bsky.social, meet researchers and call for donations-thanks for your support!

Merci!

Happy to be added too